CN-120591438-B - Molecular marker related to corn kernel starch content and application thereof

Abstract

The invention discloses a molecular marker related to corn kernel starch content and application thereof, and relates to the technical field of biology. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3. The invention successfully discovers a key gene-ZmEnd 1 for regulating and controlling the corn starch content for the first time, and discovers two key SNP loci, namely SNP-4-245033385 and SNP-4-245033215, which are positioned in a ZmEnd gene promoter region, wherein the two loci have obvious correlation with the corn kernel starch content. Based on the research results, the invention further develops a molecular marker closely related to the starch content of the corn kernels. By using the molecular marker, researchers can rapidly and accurately detect the hereditary characteristic of the starch content in corn kernels, thereby providing powerful technical support for breeding corn varieties with high starch content.

Inventors

• SHEN YAOU
• HOU FENGXIA
• ZHANG XIAOLEI
• ZHAO GUIXIANG
• LI QINGLIN
• MA LANGLANG

Assignees

• 四川农业大学

Dates

Publication Date

20260508

Application Date

20250529

Claims (2)

1. 1. The application of the molecular marker in identifying the corn kernel starch content character is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.3, the molecular marker comprises two SNP loci of SNP-4-245033215 and SNP-4-245033385, the SNP-4-245033215 is positioned at the 1751 th base of the nucleotide sequence, and the SNP-4-245033385 is positioned at the 1921 st base of the nucleotide sequence; the corn kernel starch content of the AA haplotype is higher than that of the GG haplotype.
2. 2. The method for identifying the starch content character of the corn kernels is characterized by comprising the following steps of: Extracting to obtain genome DNA of a sample to be detected; Amplifying the genome DNA as a template to obtain the molecular marker in claim 1, obtaining the haplotype of the sample to be detected, and judging the corn grain starch content character of the AA haplotype to be higher than that of the GG haplotype.

Description

Molecular marker related to corn kernel starch content and application thereof Technical Field The invention relates to the technical field of biology, in particular to a molecular marker related to corn kernel starch content and application thereof. Background Corn (Zea mays l.) is one of the most important food crops and is also an essential feed source and industrial feedstock. The kernel is used as the most important harvesting organ of corn plants, and the starch content of the kernel is usually more than 70% of the dry weight, and is the most important energy storage form in the kernel. In cereals such as corn and rice, the starch content not only determines the weight and volume of the grain, but also affects the processability and quality of the final product. Therefore, the method has important theoretical and practical significance for improving the corn yield and improving the grain quality by excavating related genes for regulating and controlling the grain starch content and deeply analyzing molecular mechanisms of starch synthesis and accumulation. However, traditional breeding methods rely on phenotypic selection, are limited by the time consuming, costly and environmental factor interference of grain starch content detection, and are difficult to achieve efficient and accurate genetic improvement. With the rapid development of molecular biology technology, the breeding efficiency can be remarkably improved by utilizing a molecular marking technology. Molecular marker technology is a tool that reveals genetic differences in individuals or populations of organisms at the molecular level, based on sequence differences in specific DNA sites from individual to individual. Closely linked with the target trait, the target trait can be rapidly, efficiently and accurately selected, and the breeding period is shortened. Among them, whole genome association analysis (GWAS) has been widely used in genetic basic research of complex quantitative traits of plants. Therefore, identifying and controlling the related genes of the starch content of the corn kernels and developing molecular markers thereof has important significance for corn genetic improvement. Disclosure of Invention The invention aims to provide a molecular marker related to the starch content of corn kernels and application thereof, so as to solve the problems in the prior art. By using the molecular marker, researchers can quickly and accurately detect the hereditary characteristic of the starch content in corn kernels, thereby providing powerful technical support for breeding corn varieties with high starch content. In order to achieve the above object, the present invention provides the following solutions: The invention provides a molecular marker related to corn kernel starch content, the nucleotide sequence of which is shown as SEQ ID NO.3, wherein the molecular marker comprises two SNP loci of SNP-4-245033215 and SNP-4-245033385, the SNP-4-245033215 is positioned at the 1751 th base of the nucleotide sequence, and the SNP-4-245033385 is positioned at the 1921 st base of the nucleotide sequence. The invention also provides a dCAPS primer group, which comprises an upstream primer dCAPS-F with a nucleotide sequence shown as SEQ ID NO.26, an upstream primer dCAPS-F-mutation with a nucleotide sequence shown as SEQ ID NO.27 and a downstream primer dCAPS-R with a nucleotide sequence shown as SEQ ID NO. 28. The invention also provides application of the dCAPS primer group in preparation of detection products for identifying corn kernel starch content traits. Further, the detection product is a kit. The invention also provides a detection product for identifying the starch content character of the corn kernels, which comprises the dCAPS primer group. Further, the detection product is a kit. The invention also provides application of the molecular marker in identifying corn kernel starch content traits. The invention also provides application of the dCAPS primer group in identifying corn kernel starch content traits. The invention also provides application of the detection product in identifying corn kernel starch content traits. The invention also provides a method for identifying the starch content character of the corn kernels, which comprises the following steps: Extracting to obtain genome DNA of a sample to be detected; And carrying out PCR amplification by using the genome DNA as a template and using the dCAPS primer group to obtain the haplotype of the sample to be detected, and judging the corn grain starch content character of the AA haplotype is higher than that of the GG haplotype. The invention discloses the following technical effects: The invention successfully discovers a key gene ZmEnd for regulating and controlling the content of corn starch for the first time, and the discovery provides an important clue for deeply researching the starch metabolism mechanism of corn kernels. Meanwhile, the invention also discovers two key SN