CN-120594822-B - Carboxyl polystyrene microsphere preservation solution and application

CN120594822BCN 120594822 BCN120594822 BCN 120594822BCN-120594822-B

Abstract

The invention relates to the technical field of latex microspheres, in particular to a carboxyl polystyrene microsphere preservation solution and application thereof, wherein the preservation solution comprises buffer solution, surfactant, sodium 2, 4-dichlorophenoxyacetate and preservative. The buffer solution is any one of boric acid buffer solution, tris buffer solution and carbonate buffer solution, the surfactant is a tween series nonionic surfactant, and the preservative is NaN 3 or Proclin 300. The sodium 2, 4-dichlorophenoxyacetate in the carboxyl polystyrene microsphere preservation solution has negative charges, chlorine atoms on the benzene ring are electron withdrawing groups, the oxygen atoms connected with the benzene ring and carbon hybridized with surrounding SP 2 can form p-pi conjugation effect, the electronegativity of the benzene ring is improved, the sodium 2, 4-dichlorophenoxyacetate is more easily adsorbed to the surface of the microsphere, the stability of the surface charge and the surface potential of the microsphere are maintained, and the microsphere is not easily aggregated in the process of combining an antibody.

Inventors

CHEN BEIBEI
ZHANG YUJI
WANG PENG
SHAO CHAOFENG

Assignees

南京立顶医疗科技有限公司

Dates

Publication Date: 20260508
Application Date: 20250617

Claims (4)

1. A carboxyl polystyrene microsphere preservation solution is characterized by comprising a buffer solution, a surfactant, sodium 2, 4-dichlorophenoxyacetate and a preservative, wherein the buffer solution is any one of a boric acid buffer solution, a tris buffer solution and a carbonate buffer solution, the working concentration of the buffer solution is 10mM-20mM, the surfactant is tween 20, the mass fraction of the surfactant is 0.2%, and the mass fraction of the sodium 2, 4-dichlorophenoxyacetate is 0.01% -0.015%.
2. The preservation solution of carboxyl polystyrene microsphere according to claim 1, wherein the preservative is NaN 3 or Proclin 300.
3. The preservation solution of carboxyl polystyrene microspheres according to claim 2, wherein the preservative is 0.04% by mass.
4. Use of the carboxylic polystyrene microsphere preservation solution according to any one of claims 1 to 3 for microsphere preservation and coupling of antibodies.

Description

Carboxyl polystyrene microsphere preservation solution and application Technical Field The invention relates to the technical field of latex microspheres, in particular to a carboxyl polystyrene microsphere preservation solution and application. Background The latex enhanced immunoturbidimetry is a relatively stable and accurate immunoturbidimetry technology, and the principle is that antibodies corresponding to substances to be tested are coated on latex microspheres of 10nm-1 mu m, so that the volume of antigen-antibody conjugate is increased, and after light passes through the conjugate, the intensity changes of transmitted light and scattered light are more obvious, thereby improving the sensitivity of the test. In order to improve dispersibility of microspheres, patent CN116087502a discloses a microsphere preservation solution, which comprises polyalcohol, buffering agent, protein protecting agent, high molecular polymer, nonionic surfactant and preservative, wherein the microsphere preservation solution enables microspheres to suspend and disperse through synergistic effect of polyalcohol, high molecular polymer and nonionic surfactant, and the long-term stability of microspheres is cooperatively maintained with the buffering agent and the protein protecting agent, so that accuracy of detection results is improved, and the patent is specifically directed at fluorescent coding microspheres of surface covalent crosslinking antibodies or antigens. Patent CN109370568B discloses a preservation solution for fluorescent microspheres, which comprises ethanol, buffer solution, inorganic sodium salt, saccharide, organic dispersing agent and water, wherein the ethanol, saccharide and organic dispersing agent cooperate to enhance the protection effect on the surface active groups of the fluorescent microsphere particles, and meanwhile, the inorganic sodium salt is used for adjusting the system ion concentration and the buffer solution to achieve better protection effect on the fluorescent microspheres, so that the stability of fluorescent signals and the stability of biological activity are improved. Patent CN115786323a discloses a magnetic microsphere preservation solution, which comprises acidic substances, metal salt substances and buffer solution, wherein metal ions and hydrogen ions not only effectively increase the magnetic responsiveness of magnetic beads, but also can increase the extraction performance of the magnetic microsphere preservation solution in a fast extraction procedure (especially in RNA extraction, such as RNA extraction of novel coronaviruses). The common latex microsphere with the surface carboxyl modified can be combined with antigen and antibody in a covalent coupling mode, but interaction exists among the microspheres, the microspheres are always aggregated after being placed for a long time, and the aggregation problem can be solved through means of ultrasonic cleaning, ultrasonic crushing and the like. However, in the activation by EDC/NHS and the binding of antibodies, the aggregation is caused by the change of charges on the microsphere surface, and the aggregation problem is difficult to solve by ultrasonication, thereby affecting the accuracy of experimental results. Based on this, it is extremely important to solve the problem of monodispersity after long-term storage of the microspheres and after binding of the antibody. However, there are few reports on the preservation solution of carboxyl polystyrene microsphere, so it is needed to provide a preservation solution of carboxyl polystyrene latex microsphere to improve the monodispersity of microsphere preserved for a long time and the monodispersity after binding antibody. Disclosure of Invention Aiming at the problems that the carboxyl polystyrene microsphere has poor long-term storage stability and is easy to agglutinate, the microsphere is easy to agglutinate and is difficult to disperse in the processes of EDC/NHS activation and antibody binding, and the like, the invention provides the carboxyl polystyrene microsphere preservation solution and application. In order to achieve the above purpose, the present invention provides the following technical solutions: A preservation solution for carboxyl polystyrene microsphere comprises buffer solution, surfactant, sodium 2, 4-dichlorophenoxyacetate and antiseptic. Wherein the buffer solution is any one of boric acid buffer solution, tris buffer solution and carbonate buffer solution so as to maintain the stability of the pH of the preservation solution. Wherein the working concentration of the buffer solution is 0.5mM-50mM. Further, the working concentration of the buffer is 1mM-25mM. Further, the working concentration of the buffer is 10mM-20mM. Wherein the surfactant is a tweens series nonionic surfactant, preferably one of tweens 20 and tweens 80. Wherein the mass fraction of the surfactant is 0.01% -0.5%. Further, the mass fraction of the surfactant is 0.05% -0.3%. Further, t