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CN-120616838-B - Method for constructing mouse model for inducing acute inflammatory anemia

CN120616838BCN 120616838 BCN120616838 BCN 120616838BCN-120616838-B

Abstract

The invention provides a method for constructing a mouse model for inducing acute inflammatory anemia. The existing inflammatory anemia model is long in construction period and cannot simulate the clinical phenomenon of acute anemia, and the mouse model provided by the invention can induce the number of red blood cells and the level of hemoglobin to be obviously reduced through single Poly (I: C) stimulation for 36 hours, so that the acute anemia time window is accurately simulated, and a good foundation is laid for the mechanism research of acute inflammatory anemia.

Inventors

  • LIU DEPEI
  • WANG HEPING
  • LIU XINGYI
  • CUI SHENSHEN
  • WEI ZIYU

Assignees

  • 中国医学科学院基础医学研究所

Dates

Publication Date
20260512
Application Date
20250808

Claims (7)

  1. 1. A method for constructing a mouse model for inducing acute inflammatory anemia is characterized by comprising the following steps of singly administering Poly (I: C) to a C57BL/6J mouse, wherein the administration method is intraperitoneal injection, the administration dosage is 12 mg/kg, and the model construction is completed after 36 hours.
  2. 2. Use of a mouse model obtained by the construction method of claim 1 in research of pathogenesis of acute inflammatory anemia.
  3. 3. The use of the mouse model obtained by the construction method of claim 1 in screening acute inflammatory anemia therapeutic drugs.
  4. 4. A method of screening for a drug for treating acute inflammatory anemia, the method comprising the steps of: administering a drug to be screened to a mouse model obtained by the construction method of claim 1; Detecting at least one index of the mouse peripheral blood mononuclear cell proportion, the erythrocyte concentration, the hemoglobin concentration, the spleen weight, the hemoglobin gene expression level, the serum Erythropoietin (EPO) level, the serum inflammatory factor level, the innate immunity gene and the erythroid regulation gene expression level; Comparing the detection results of the mice to which the drug to be screened is applied and the mice to which the drug to be screened is not applied, and evaluating whether the drug to be screened is the therapeutic drug for acute inflammatory anemia.
  5. 5. The method of claim 4, wherein the serum inflammatory factor is TNF- α and/or IL-6.
  6. 6. The method of claim 4, wherein the drug to be screened comprises a protein analog, an antibody, DNA, RNA, a small molecule compound.
  7. 7. The method of claim 4, wherein the drug to be screened comprises a drug or a pharmaceutical composition for separate use.

Description

Method for constructing mouse model for inducing acute inflammatory anemia Technical Field The invention relates to the field of biological medicine, in particular to a method for constructing a mouse model for inducing acute inflammatory anemia. Background Inflammatory anemia (anemia of inflammation, AI), a common anemia condition, severely affects the health and quality of life of the patient. In AI patients, 71% suffer from acute infection, 12% from cancer, 16% from chronic infection or autoimmune disease. According to the pathogenesis and etiology, the inflammatory anemia can be classified into various types, wherein the acute inflammatory anemia is relatively common in clinic, is usually caused by acute inflammatory reactions such as infection, trauma, operation and the like, and is usually sudden within 24-48 hours, and the acute inflammatory anemia is characterized by sudden onset, and can cause serious consequences such as multiple organ dysfunction if the acute inflammatory anemia cannot be effectively intervened in time. Therefore, intensive research into pathogenesis of acute inflammatory anemia and development of effective therapeutic drugs are of great clinical significance. The existing anemia model construction method comprises gene editing, chemical or physical induction and the like, but has obvious defects in the aspect of constructing related researches of acute inflammatory anemia, such as complex operation, high time cost, unstable phenotype and the like. For example, the genetic anemia is mainly simulated by the anemia model constructed by gene editing, the pathogenesis of the genetic anemia is different from that of infectious anemia, and a proper model foundation cannot be provided for the research of acute inflammatory anemia. The chronic inflammatory anemia phenotype can be generated by continuous multiple times of stimulation (for example, more than 7 days) by using an LPS-induced anemia model, and has long time consumption and multiple interference factors. At present, a method for quickly and stably constructing the acute inflammatory anemia model is lacking, so that the time node of the acute inflammatory anemia is defined, and powerful support is provided for quick screening of acute inflammatory anemia treatment medicines and deep research of pathogenesis. Based on the background, the development of an effective mouse model construction method for inducing acute inflammatory anemia has important clinical significance and application value. Disclosure of Invention Aiming at the problems that the acute inflammatory anemia model cannot be quickly and stably built and the time node for defining the acute inflammatory anemia is lacking in the prior art, the invention provides a mouse model building method which is different from the prior art and is used for inducing the acute inflammatory anemia through single Poly (I: C) induction and time window control. In order to achieve the above purpose, the specific technical scheme provided by the invention is as follows: In a first aspect, the invention provides a method for constructing a mouse model for inducing acute inflammatory anemia, the method comprising the steps of administering Poly (I: C) to a mouse in a single time, and completing the model construction after 36 hours. In the present invention, acute inflammatory anemia refers to a secondary anemia state caused by an acute disease (e.g., infection, trauma, surgery, etc.), the core mechanism of which is associated with an acute inflammatory response, immune activation, or tissue damage. Such anemia is a sudden onset characterized by a short-term decrease in hemoglobin concentration, often accompanied by fluctuations in the course of the primary disease. Pathophysiological processes are mainly involved in inflammatory cytokine mediated myelosuppression, reduced hemoglobin gene expression, and acceleration of erythrocyte destruction. In the present invention, poly (I: C) (Polyinosinic acid-polycytidylic acid) is also known as (PolyI: C), and the Chinese alias includes polycytidylic acid, polyinosinic acid cytosine nucleotide, polyinosinic acid-polycytidylic acid, and CAS number is 42424-50-0. Further, the administration dose of Poly (I: C) was 12 mg/kg. Further, the method of administration of Poly (I: C) is intraperitoneal injection. Further, the mice were C57BL/6J mice. In a second aspect, the invention provides a mouse model obtained by the construction method according to the first aspect of the invention. In some embodiments, the detection result of the mouse model is that there is a change in at least one indicator of an increase in peripheral blood mononuclear cell ratio, a decrease in red blood cell concentration, a decrease in hemoglobin concentration, an increase in spleen weight, a decrease in hemoglobin gene expression level, an increase in innate immunity gene expression level, and a decrease in erythroid regulator gene expression level, as compared to a wild-type