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CN-120624214-B - Alternaria leucoderma DCY6478 and culture medium and application thereof

CN120624214BCN 120624214 BCN120624214 BCN 120624214BCN-120624214-B

Abstract

The invention discloses a white micro-skin umbrella DCY6478, a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a white micro-skin umbrella (Marasmiellus sp.) DCY6478 with the preservation number of GDMCC No.65883. Experiments prove that the culture medium formula most suitable for the growth of the white micro-skin umbrella comprises 40 weight percent of apple sawdust, 36 weight percent of corncob, 14 weight percent of cotton seed hulls, 1 weight percent of lime and 1 weight percent of gypsum, the fermentation liquor has an inhibition effect on various pathogenic bacteria, the DPPH free radical scavenging capability, the superoxide dismutase activity and the catalase activity of fermentation hypha are higher than those of fruiting bodies, the culture medium can be used as a natural antioxidant source, the fermentation hypha or the fruiting bodies have the capability of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries such as food, medicine and the like.

Inventors

  • XIANG ZHUN
  • DENG CHUNYING
  • Yang Yunshen
  • LIN QUNYING
  • WANG WANKUN
  • ZHENG XUAN
  • HE YAOWEI

Assignees

  • 贵州省生物研究所
  • 中华全国供销合作总社南京野生植物综合利用研究所

Dates

Publication Date
20260512
Application Date
20250526

Claims (7)

  1. 1. DCY6478 was deposited with the microorganism seed group of Guangdong province under the accession number GDMCC No.65883 and the date of deposition was 2025, 2 months and 14 days.
  2. 2. Use of Bai Wei umbrella (Marasmiellus sp.) DCY6478 according to claim 1 for the cultivation of fruit bodies of micro-picoumbrellas.
  3. 3. Use of fermentation broth of Fusarium graminearum (Marasmiellus sp.) DCY6478 for inhibiting growth of pathogenic bacteria, which are Fusarium solani, fusarium oxysporum Fusarium oxysporum, penicillium aurantiacum Penicillium aurantiogriseum, and phoma chufa Phoma bellidis.
  4. 4. Use of fermentation mycelium of DCY6478 of micro-umbrella (Marasmiellus sp.) in the preparation of antioxidant.
  5. 5. Use of a fermented mycelium or fruiting body of DCY6478 in the production of amino acids.
  6. 6. Use of fermented mycelium or fruiting body of DCY6478 in the production of polysaccharides.
  7. 7. Use of a fermented mycelium or fruiting body of DCY6478 in the production of crude protein.

Description

Alternaria leucoderma DCY6478 and culture medium and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a micro-skin umbrella DCY6478, a culture medium and application thereof. Background The micro-skin umbrella (Marasmiellus candidus) belongs to the genus micro-skin umbrella Marasmiellus Murrill of basidiomycete Basidiomycota, agaricales AGARICALES and small Pi Sanke Omphalotaceae, and is a type of large fungus which is common in subtropical areas. The fungus is used as an important pathogen of subtropical plants and medicinal fungi, and has potential research and application values in the fields of ecological systems and medicines. However, the current study on the micro-picoumbrella genus starts late, and has insufficient attention, and a plurality of problems such as disordered school names, serious false identification and the like exist. In natural environment, the Umbelliferae is often decomposed in sapropel and plant humus, and the growth environment is easily interfered by external factors. In recent years, due to ecological environment destruction caused by human activities, forest felling, habitat fragmentation and the like are increasingly serious, and the natural growth space of the white micro-skin umbrella is greatly compressed. At the same time, the behavior of overdriving is also rampant. On one hand, partial personnel acquire the micro-skin umbrella in the wild without control due to insufficient ecological value understanding of the micro-skin umbrella so as to meet the requirements of medical use under collection or error cognition, and on the other hand, the requirements of specimens and research materials of the micro-skin umbrella are increased along with the gradual development of the research of the micro-skin umbrella, so that the field acquisition pressure is further increased. The wild micro-picoumbrella resource is drastically reduced, the survival crisis is faced, the population quantity is difficult to maintain stable, and even the linkage influence on the material circulation and the energy flow of the ecological system where the wild micro-picoumbrella resource is located is possible. From the artificial cultivation point of view, the research and practice of the artificial cultivation of the white micro-skin umbrella are seriously lagged. Because the naturally collected micro-phloem is often decomposed in a complex environment, the identification of a definite host is very difficult, so that a proper culture medium is difficult to obtain for culturing to form an artificial sporophore. Meanwhile, the white micro-skin umbrella also often appears in the form of endophytes, and fruiting bodies are not naturally formed in an endophyte state, which brings great challenges to artificial cultivation. At present, the disclosure report on the artificial cultivation method of the white micro-picoumbrella is almost blank, and in the related research of the existing micro-picoumbrella, only a few cultivation researches of other types of micro-picoumbrella, such as Marasmiellus inoderma as pathogenic bacteria are cultivated and domesticated in oil palm, and Marasmiellus scandens is endophytic fungi separated and purified from agalloch eaglewood (Aquilaria sinensis) and domesticated. Under such circumstances, research on the artificial cultivation method of the micro-skin umbrella is being carried out. Disclosure of Invention Aiming at the problems in the prior art, the technical problem to be solved by the invention is to provide a white micro-skin umbrella (Marasmiellus sp.) DCY6478. The invention also solves the technical problem of providing a culture medium for cultivating the white micro-skin umbrella. Yet another technical problem to be solved by the present invention is to provide the use of DCY6478 for cultivating the fruiting body of micro-skinned umbrella (Marasmiellus sp.). In order to solve the technical problems, the technical scheme adopted by the invention is as follows: DCY6478 was deposited with the microorganism seed group of Guangdong province under the accession number GDMCC No.65883 and the date of deposition was 2025, 2 months and 14 days. Use of umbrellas (Marasmiellus sp.) DCY6478 in the cultivation of fruiting bodies of umbrellas. A culture medium for culturing the DCY6478 of the micro-skin umbrella comprises, by weight, 40% of wood dust, 36% of corncob, 14% of cotton seed hulls, 1% of lime and 1% of gypsum. The wood chips comprise apple wood chips, or medium-sized and small She Qinggang wood chips, or agilawood wood chips. Use of fermentation broth of DCY6478 of micro-umbrella (Marasmiellus sp.) for inhibiting the growth of pathogenic bacteria. The pathogenic bacteria are Fusarium solani, fusarium oxysporum Fusarium oxysporum, penicillium aureum Penicillium aurantiogriseum and Phoma chufa Phoma bellidis. Use of fermentation mycelium of DCY6478 of micro-umbrella (Marasmiellus sp.) in the