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CN-120666032-B - SNP molecular marker related to chicken egg yield and application thereof

CN120666032BCN 120666032 BCN120666032 BCN 120666032BCN-120666032-B

Abstract

The invention provides an SNP molecular marker related to chicken egg production number and application thereof, belonging to the technical field of molecular genetics and animal breeding, wherein the SNP molecular marker is positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence, corresponds to 136 th bp in the sequence shown in SEQ ID NO.1, and the base is C or T. The SNP molecular marker can be used for early breeding selection of chicken egg laying number, effectively improves average egg laying number of chicken flocks, reduces breeding cost, and provides a convenient technical means for establishing molecular marker assisted breeding for chicken egg laying number selection.

Inventors

  • SHAO DAN
  • Luo Yangqiwen
  • Li Ashi
  • QU LIANG
  • LU JIAN
  • GUO JUN
  • DOU TAOCUN
  • WANG QIANG
  • LI YONGFENG
  • GUO WEI
  • SHEN MANMAN

Assignees

  • 江苏省家禽科学研究所
  • 江苏科技大学

Dates

Publication Date
20260508
Application Date
20250527

Claims (8)

  1. 1. The application of the SNP molecular marker related to the chicken egg laying number in genetic breeding for improving chicken egg laying performance is characterized in that the SNP molecular marker is positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence, corresponds to 136 th bp in the sequence shown in SEQ ID NO.1, and is C or T; the chicken is of a green-shell layer chicken variety.
  2. 2. An early selection method of chicken egg yield traits, which is characterized by comprising the steps of early selecting chicken egg yield traits based on the genotype of SNP molecular markers related to chicken egg yield; the SNP molecular marker is positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence, corresponds to 136bp in the sequence shown in SEQ ID NO.1, and the base is C or T; the chicken is of a green-shell layer chicken variety.
  3. 3. The method for early selection of chicken egg laying number traits according to claim 2, wherein the early selection method specifically comprises: detecting the genotype of the SNP molecular marker in the genome of the chicken to be detected; Early selecting the egg laying number character of the chicken to be detected based on the genotype of the SNP molecular marker; Wherein the number of eggs laid by the CC genotype group of the SNP molecular marker is larger than that of eggs laid by the TT or CT genotype group, the CC genotype group is reserved in the early selection process, and the TT and CT genotype groups are eliminated.
  4. 4. The method for early selection of chicken egg yield traits according to claim 3, wherein said detecting the genotype of said SNP molecular marker in the genome of a chicken to be detected comprises: carrying out PCR amplification on the genomic DNA of the chicken to be detected by using the primer F and the primer R; sequencing the PCR amplification product to obtain the genotype of the SNP molecular marker in the chicken genome to be detected; The nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3.
  5. 5. The application of a detection primer of SNP molecular markers related to chicken egg production in genetic breeding for improving chicken egg production performance is characterized in that the SNP molecular markers are positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence and correspond to 136 th bp in a sequence shown in SEQ ID NO.1, and the base is C or T; the chicken is of a green-shell layer chicken variety.
  6. 6. The use according to claim 5, wherein the detection primer comprises a primer F and a primer R, the nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3.
  7. 7. The application of the SNP molecular marker detection kit related to the chicken egg production number in genetic breeding for improving chicken egg production performance is characterized in that the SNP molecular marker is positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence, corresponds to 136 th bp in the sequence shown in SEQ ID NO.1, and is characterized in that the base is C or T; the chicken is of a green-shell layer chicken variety.
  8. 8. The use according to claim 7, wherein the detection kit comprises a primer F and a primer R, the nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3.

Description

SNP molecular marker related to chicken egg yield and application thereof Technical Field The invention belongs to the technical fields of molecular genetics and animal breeding, and particularly relates to an SNP molecular marker related to chicken egg production and application thereof. Background The laying hen industry in China is the biggest global production and consumption market, and provides a high-quality protein source for improving the national dietary structure. Genetic improvement of egg production performance in the breeding process is still a core target of seed source innovation in the laying hen industry. The egg yield is the most important index affecting the economic character of chickens, the genetic force parameter is only 0.18-0.22, and under the existing breeding conditions, a plurality of breeding institutions still adopt the traditional phenotype selection technology, the annual genetic progress is less than 1.5, so that the genetic progress is slow, and the market competitiveness of the independently developed varieties in China is severely restricted. Disclosure of Invention In order to genetically improve the egg yield of the laying hens, the invention provides an SNP molecular marker related to the egg yield of the chickens, and the SNP molecular marker is positioned in an ER alpha gene, can be used for carrying out early breeding selection on the egg yield of the chickens, effectively improves the average egg yield of chicken flocks and reduces the breeding cost. The invention also provides application of the SNP molecular marker related to the chicken egg laying number in genetic breeding for improving chicken egg laying performance. The invention is realized by the following technical scheme: The invention provides an SNP molecular marker related to chicken egg production number, which is positioned at 1141 th site of chicken genome ER alpha gene cDNA sequence and corresponds to 136 th bp in the sequence shown in SEQ ID NO.1, wherein the base is C or T. Based on the same inventive concept, the invention provides an application of SNP molecular markers related to chicken egg production number in genetic breeding for improving chicken egg production performance. Based on the same conception, the invention provides an early selection method of chicken egg laying number traits, which comprises the step of early selecting the chicken egg number traits based on the genotype of the SNP molecular marker related to the chicken egg number. Further, the early selection method specifically includes: detecting the genotype of the SNP molecular marker in the genome of the chicken to be detected; Early selecting the egg laying number character of the chicken to be detected based on the genotype of the SNP molecular marker; Wherein the number of eggs laid by the CC genotype group of the SNP molecular marker is larger than that of eggs laid by the TT or CT genotype group, the CC genotype group is reserved in the early selection process, and the TT and CT genotype groups are eliminated. Further, the detecting the genotype of the SNP molecular marker in the genome of the chicken to be detected specifically comprises: carrying out PCR amplification on the genomic DNA of the chicken to be detected by using the primer F and the primer R; sequencing the PCR amplification product to obtain the genotype of the SNP molecular marker in the chicken genome to be detected; The nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3. Based on the same inventive concept, the invention provides application of a detection primer of SNP molecular markers related to chicken egg production number in genetic breeding for improving chicken egg production performance. Further, the detection primer comprises a primer F and a primer R, the nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3. Based on the same inventive concept, the invention provides an application of a detection kit of SNP molecular markers related to chicken egg production in genetic breeding for improving chicken egg production performance. Further, the detection kit comprises a primer F and a primer R, wherein the nucleotide sequence of the primer F is shown as SEQ ID NO.2, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 3. One or more technical solutions in the embodiments of the present invention at least have the following technical effects or advantages: 1. The SNP molecular marker related to the chicken egg yield is C/T mutation of 1141 th locus of a chicken genome ER alpha gene cDNA sequence, is related to the chicken egg yield, can be used for early breeding selection of chicken egg yield traits, effectively improves average egg yield of chicken flocks and reduces breeding cost. 2. The SNP molecular marker related to the chicken egg laying number is applied to genetic br