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CN-120699771-B - Layered stem cell incubator

CN120699771BCN 120699771 BCN120699771 BCN 120699771BCN-120699771-B

Abstract

The invention relates to the technical field of stem cell culture, in particular to a layered stem cell incubator which comprises an incubator body, wherein a placement cavity is formed in the incubator body, a chamber door is hinged to the opening of the placement cavity, the layered stem cell incubator further comprises a limiting mechanism and an anti-drop mechanism, the limiting mechanism is used for limiting a round vessel, damage caused by sliding or dropping of the vessel when articles in the incubator are carried and stored and taken out can be effectively avoided through limiting the limiting mechanism, the anti-drop mechanism is used for preventing the layered placement plate from dropping when the layered placement plate is pulled and pulling, the layered placement plate is prevented from being separated from the placement cavity of the incubator body when being pulled too fast, a connecting shaft slides along the anti-drop groove when an experimenter pulls the layered placement plate, the connecting shaft is automatically embedded into a corrugated groove of the anti-drop groove when stopped, and the layered plate is prevented from completely separating from the placement cavity due to overstrong force or misoperation, and the falling damage of the culture vessel is prevented.

Inventors

  • CHEN YI
  • JIN XUEMEI
  • SUN MINGHUI
  • LIU YANRAN
  • TENG TENG

Assignees

  • 卡森(吉林)细胞资源库管理有限公司

Dates

Publication Date
20260512
Application Date
20250707

Claims (7)

  1. 1. The layered stem cell incubator comprises an incubator body (1), wherein the incubator body (1) is provided with a placement cavity (2), and a door (3) is hinged at the opening of the placement cavity (2), and is characterized by further comprising: The limiting mechanism (9), the limiting mechanism (9) is arranged in the placing cavity (2), the limiting mechanism (9) comprises layered placing plates (10) which are equidistantly arranged in the placing cavity (2), a first limiting plate (18) and a second limiting plate (19) are arranged on the layered placing plates (10), the limiting mechanism (9) is used for limiting the culture vessel, the limiting mechanism (9) further comprises a straight slot (11) which is formed in the layered placing plates (10) in a penetrating mode, a fixed shaft (12) is fixedly arranged at the top of the layered placing plates (10), a rotating plate (16) is arranged on the outer surface of the fixed shaft (12) in a rotating mode, and resistance is arranged between the rotating plate (16) and the fixed shaft (12) in a rotating mode; anti-drop mechanism (22), anti-drop mechanism (22) set up in stop gear (9) both sides, anti-drop mechanism (22) are including setting up fixed strip (23) in layering place board (10) both sides bottom, draw-in groove (24) have been seted up at fixed strip (23) top, the bottom of layering place board (10) slides in draw-in groove (24), anti-drop mechanism (22) are used for preventing layering place board (10) pull time to drop, anti-drop mechanism (22) still including seting up spout (25) on fixed strip (23), spout (25) and draw-in groove (24) intercommunication each other, be provided with slider (26) in spout (25), the top of slider (26) is with the bottom fixed connection of layering place board (10), the symmetry is fixedly provided with connecting strip (27) on slider (26), slider (26) are passed through connecting strip (27) and are slided in spout (25), run through on fixed strip (23) and are equipped with anti-drop groove (28).
  2. 2. The layered stem cell incubator according to claim 1, wherein a ventilation opening (4) is formed in one side of the incubator body (1), a support (5) is fixedly arranged at the bottom of the incubator body (1), a sealing strip (6) is fixedly arranged at one side of an opening of the placement cavity (2), a handle (7) is fixedly arranged at one side of the refrigerator door (3), and a control panel (8) is arranged at one side of the refrigerator door (3) close to the handle (7).
  3. 3. The layered stem cell incubator according to claim 1, wherein a fixed disc (13) is fixedly arranged at the top of the rotating disc (16), a limiting groove (14) is formed in the bottom of the fixed disc (13), one section of the limiting groove (14) is concavely arranged towards the circle center of the fixed disc (13), a roll shaft (15) is slidably arranged in the limiting groove (14), and the bottom of the roll shaft (15) extends out of the limiting groove (14).
  4. 4. A layered stem cell incubator according to claim 3, wherein a plurality of groups of guide rails (17) are symmetrically arranged on the rotating disc (16), the plurality of groups of guide rails (17) are arranged in a circumferential array, the guide rails (17) are fixedly connected with the first limiting disc (18), and the guide rails (17) are slidably connected with the second limiting disc (19).
  5. 5. The layered stem cell incubator according to claim 4, wherein the first limiting plate (18) and the second limiting plate (19) are provided with arc grooves (20) in a penetrating manner, the arc grooves (20) on the first limiting plate (18) and the second limiting plate (19) are arranged oppositely, one side of the second limiting plate (19) is fixedly provided with a connecting rod (21), and one end, far away from the second limiting plate (19), of the connecting rod (21) is fixedly connected with the roller shaft (15).
  6. 6. The layered stem cell incubator according to claim 1, wherein the sliding bar (26) is symmetrically and fixedly provided with a connecting shaft (29), the connecting shaft (29) is rotatably provided with a connecting sheet (30), one end of the connecting sheet (30) far away from the connecting shaft (29) is fixedly provided with a moving shaft (31), and the moving shaft (31) is slidably arranged in the anti-falling groove (28).
  7. 7. The layered stem cell incubator according to claim 6, wherein grooves (32) are formed in the connecting pieces (30), notches (33) are formed in one side of the grooves (32) located on the connecting pieces (30), elastic pieces (34) are movably arranged in the grooves (32), fixing blocks (35) are fixedly arranged on two sides of one end, far away from the grooves (32), of each elastic piece (34), and the fixing blocks (35) are fixedly connected with the side walls of the connecting pieces (30).

Description

Layered stem cell incubator Technical Field The invention relates to the technical field of stem cell culture, in particular to a layered stem cell incubator. Background The stem cell incubator is special equipment for maintaining survival, proliferation and differentiation of stem cells, simulates in-vivo physiological environment by accurately controlling environmental parameters such as temperature, humidity, gas concentration and the like, ensures the stability of biological characteristics of the stem cells, and is core equipment in the fields of stem cell basic research, clinical treatment, drug development and the like. After the power supply of the equipment is connected, the temperature and the concentration of CO 2 are set, then the culture vessel paved with matrigel is put into an incubator for pre-incubation, when cells are recovered, the freezing tube is quickly melted, and after centrifugation, the culture vessel is resuspended in a complete culture medium and inoculated into the pretreated culture vessel. The stem cell culture vessel can be a round plastic vessel which is suitable for cell growth and propagation, is particularly convenient for observing and selecting single cell colonies, has a large opening and is convenient to operate, the culture vessel is directly placed on a placing frame, and then the culture vessel is pushed into an incubator for culture. Because the contact area of the round vessel and the placing frame is smaller, if the strength is uneven or the operation is too fast when the round vessel is pushed into the incubator, the vessel is easy to incline and slide, even the culture solution overflows, and pollution or cell damage can be caused. In addition, place between board and the incubator and carry out spacingly through outstanding spacing, exert effort too big when the pull is placed the board and cause and place the board and break away from the placing cavity of incubator fast, the cultivation household utensils produces violently to rock because of inertia, probably leads to the culture solution to spill over outside the household utensils, splash into the incubator inside even. The spilled culture fluid may not only contaminate other samples, but may also cause loss of cell number or local osmotic pressure changes, affecting stem cell activity. Disclosure of Invention The invention aims to provide a layered stem cell incubator, which solves the problems set forth in the background art. The technical scheme adopted by the application for solving the technical problems is that the layered stem cell incubator comprises an incubator body, wherein the incubator body is provided with a placing cavity, and a door is hinged at the opening of the placing cavity, and the layered stem cell incubator further comprises: The limiting mechanism is arranged in the placing cavity and comprises layered placing plates which are equidistantly arranged in the placing cavity, a first limiting plate and a second limiting plate are arranged on the layered placing plates, and the limiting mechanism is used for limiting the culture vessel; The anti-falling mechanism is arranged on two sides of the limiting mechanism and comprises fixing strips arranged at two sides of the layered placing plate, clamping grooves are formed in the tops of the fixing strips, the bottoms of the layered placing plate slide in the clamping grooves, and the anti-falling mechanism is used for preventing the layered placing plate from falling off when being pulled. Preferably, the vent has been seted up to incubator body one side, incubator body bottom is fixed to be provided with the support, place chamber opening part one side and fixedly be provided with the sealing strip, chamber door one side is fixed to be provided with the handle, the chamber door is close to one side of handle and is provided with control panel. Preferably, the limiting mechanism further comprises a straight slot penetrating through the layered placing plate, the top of the layered placing plate is fixedly provided with a fixed shaft, the outer surface of the fixed shaft is rotatably provided with a rotating disc, and resistance is arranged between the rotating disc and the fixed shaft in a rotating mode. Preferably, the fixed disk that is provided with in rolling disc top, the spacing groove has been seted up to the fixed disk bottom, spacing groove one section is the sunken setting in fixed disk centre of a circle department, the sliding of spacing groove is provided with the roller, the spacing groove is extended to the roller bottom. Preferably, the rotating disc is fixedly provided with a plurality of groups of guide rails which are symmetrically arranged, the guide rails are arranged in a circle, the guide rails are fixedly connected with the first limiting disc, and the guide rails are connected with the second limiting disc in a sliding manner. Preferably, the first limiting plate and the second limiting plate are respectively pro