CN-120713863-B - Antibacterial synergistic-mineralization regulation dual-function nano material and preparation method and application thereof

Abstract

The invention provides an antibacterial synergistic-mineralization regulation dual-function nano material and a preparation method and application thereof, belonging to the technical field of biomedical materials. The preparation method comprises the steps of firstly mixing a disodium hydrogen phosphate solution and a calcium chloride solution to obtain an amorphous calcium phosphate suspension, then mixing the amorphous calcium phosphate suspension and a tannic acid solution to obtain an ACP@TA suspension, and finally mixing the ACP@TA suspension and a hydroxypropyl trimethyl ammonium chloride chitosan solution to obtain the difunctional nanomaterial. The crosslinked shell is formed by the electrostatic adsorption of tannic acid and the hydroxypropyl trimethyl ammonium chloride chitosan serving as an antibacterial layer, so that the amorphous calcium phosphate is effectively wrapped, the crystallization process of the amorphous calcium phosphate can be obviously delayed, the release of long-acting calcium phosphate ions is ensured, the antibacterial function can be effectively exerted, an ideal local microenvironment is provided for remineralization of dental hard tissues, and the biomimetic mineralization of dental enamel and the deep mineralization blocking of dentin tubules are finally realized.

Inventors

• QIU RONGMIN
• LI YUYAN
• Xie yulu
• Ran Lingshan
• GAO WANSHAN
• LUO JUN
• LI JIANSHU
• Lan Jue
• DENG SICHENG

Assignees

• 广西医科大学

Dates

Publication Date

20260508

Application Date

20250708

Claims (8)

1. 1. The preparation method of the antibacterial synergistic-mineralization regulation dual-function nano material is characterized by comprising the following steps of: (1) Mixing disodium hydrogen phosphate solution and calcium chloride solution to obtain amorphous calcium phosphate suspension; (2) Mixing amorphous calcium phosphate suspension and tannic acid solution to obtain ACP@TA suspension; (3) Mixing ACP@TA suspension and hydroxypropyl trimethyl ammonium chloride chitosan solution to obtain a bifunctional nano material; the concentration of the tannic acid solution in the step (2) is 6-12 mg/mL, and the pH value is 8; And (3) in the step (2), the volume ratio of the amorphous calcium phosphate suspension to the tannic acid solution is 60:0.10-0.14.
2. 2. The preparation method of the antibacterial synergistic-mineralization regulation dual-function nano material according to claim 1, wherein in the step (1), the molar ratio of Ca in a calcium chloride solution to P in a disodium hydrogen phosphate solution is 1.2-1.5:1, the concentration of the disodium hydrogen phosphate solution is 0.002-0.006mol/L, and the concentration of the calcium chloride solution is 0.004-0.008 mol/L.
3. 3. The method for preparing the antibacterial synergistic-mineralization control dual-functional nano material according to claim 2, wherein in the step (2) and the step (3), the mixing temperature is independently 0-6 ℃, and the mixing time is independently 3-8 min.
4. 4. The method for preparing the antibacterial synergistic-mineralization regulation dual-function nano-material according to claim 1, wherein the concentration of the hydroxypropyl trimethyl ammonium chloride chitosan solution in the step (3) is 6-15 mg/mL.
5. 5. The method for preparing the antibacterial synergistic-mineralization control dual-function nano material according to claim 2 or 4, wherein the volume ratio of the amorphous calcium phosphate suspension to the hydroxypropyl trimethyl ammonium chloride chitosan solution is 60:3-5.
6. 6. The method for preparing the antibacterial synergistic-mineralization regulation dual-function nano-material according to claim 1, wherein in the step (3), the hydroxypropyl trimethyl ammonium chloride chitosan solution is added into the ACP@TA suspension in batches during mixing, and incubated for 3-8 min after each addition.
7. 7. The method for preparing the antibacterial synergistic-mineralization control dual-functional nanomaterial according to any one of claims 1 to 6.
8. 8. Use of the bifunctional nanomaterial of claim 7, for preparing a tooth remineralizing product or an anti-dentinal sensitivity product.

Description

Antibacterial synergistic-mineralization regulation dual-function nano material and preparation method and application thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to an antibacterial synergistic-mineralization regulation dual-function nano material and a preparation method and application thereof. Background Caries is the third most chronic non-infectious disease listed by world health organization as a secondary cancer, cardiovascular disease, severely affecting human oral health and physical and mental health. In recent years, stopping caries progression and repairing defective hard tissues by remineralization methods has become a popular research direction for oral treatment. Remineralization treatment refers to a treatment method that remineralizes demineralized enamel or cementum with a remineralizing solution, restores its hardness, and terminates or eliminates early caries lesions. With the continuous and intensive research, the application content of the dental caries is gradually changed from early caries to experimental treatment of caries at other stages. The remineralization treatment principle is that neutral solution containing a certain proportion of calcium and phosphorus ions is delivered to a hard tissue demineralization area, and mineral ions with relatively high concentration in the solution are mineralized and accumulated in situ to fill demineralization gaps, so that the defect micro-area is repaired. The mineralization liquid needs to remain on the tooth surface for a certain period of time to facilitate the stable supply of calcium phosphate ions to promote the mineralization progress, meanwhile, the partial environment pH value required in the remineralization solid phase conversion process, namely the process of converting amorphous calcium phosphate into hydroxyapatite and continuing the growth is about 6.2, the intake of high sugar or carbohydrate and the like can cause active metabolism of cariogenic bacteria in the oral cavity, the produced large amount of organic acid can easily change the environment pH value, the mineralization-demineralization dynamic balance on the tooth surface is broken, and finally, the tooth hard tissue is gradually destroyed and caries is caused to progress. Therefore, the high-efficiency remineralization treatment needs to provide high-concentration calcium and phosphorus ions for the tooth demineralization area to realize long-acting mineralization, and meanwhile realizes effective local antibacterial effect of the tooth hard tissue while promoting remineralization so as to prevent demineralization aggravation and remineralization inhibition caused by continuous acidogenesis of cariogenic bacteria. Therefore, research on an antibacterial synergistic-mineralization regulation dual-function nano material and a preparation method thereof is of great significance in products for remineralization and antibacterial treatment of dental hard tissues. Disclosure of Invention The invention aims to provide an antibacterial synergistic-mineralization regulation dual-function nano material, and a preparation method and application thereof, so as to solve the problem of caries progression caused by a remineralization process in the prior art. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a preparation method of an antibacterial synergistic-mineralization regulation dual-function nano material, which comprises the following steps: (1) Mixing a disodium hydrogen phosphate solution and a calcium chloride solution to obtain an amorphous calcium phosphate (amorphous calciumphosphate, ACP) suspension; (2) Mixing an amorphous calcium phosphate suspension with a tannic acid (TANNIC ACID, TA) solution to obtain an ACP@TA suspension; (3) Mixing the ACP@TA suspension and a hydroxypropyl trimethyl ammonium chloride chitosan (hydroxypropyltrimethyl ammonium chloride chitosan, HACC) solution to obtain the double-function nano material (ACP@TH). Preferably, in the step (1), the molar ratio of Ca in the calcium chloride solution to P in the disodium hydrogen phosphate solution is 1.2-1.5:1, the concentration of the disodium hydrogen phosphate solution is 0.002-0.006mol/L, and the concentration of the calcium chloride solution is 0.004-0.008 mol/L. Preferably, in the step (2), the concentration of the tannic acid solution is 6-12 mg/mL, and the pH value is 8. Preferably, in the step (2), the volume ratio of the amorphous calcium phosphate suspension to the tannic acid solution is 60:0.10-0.14. Preferably, in the step (2) and the step (3), the temperature of the mixing is independently 0-6 ℃, and the mixing time is independently 3-8 min. Preferably, in the step (3), the concentration of the hydroxypropyl trimethyl ammonium chloride chitosan solution is 6-15 mg/mL. Preferably, the volume ratio of the amorphous calcium phosphate suspension