CN-120758361-B - Mucor racemosus DLS157 and application thereof

Abstract

The invention discloses a Mucor racemosus DLS157 and application thereof, belonging to the technical field of microorganisms. The preservation number of the strain is CGMCC No.41738, the strain has strong capability of fermenting lignocellulose to produce humic acid, and DLS157 bacterial manure is prepared by fermenting rice straw powder and wheat bran with the strain. After the DLS157 bacterial manure is added into soil, wheat and Chinese cabbage are sowed, so that the agronomic characters such as plant height, leaf length, leaf width and leaf number of the wheat are obviously promoted, and the biomass such as plant height, leaf width, leaf number, fresh weight on the ground and dry weight on the ground of the Chinese cabbage are also obviously improved. Through research on the soil after wheat sowing is finished, the addition of the DLS157 bacterial fertilizer obviously changes the soil microbial community, increases the diversity and abundance of the soil microbial community and improves the content of humus in the soil. Further illustrates that the DLS157 bacterial fertilizer is beneficial to the regulation of the soil microbial community structure and can be used for soil improvement. Has important significance for realizing the resource utilization of the agricultural straw.

Inventors

• Yin Yirui
• YIN XINLONG
• CHEN MINGHUI
• XIE KAIQING
• ZHU DAN
• YANG ZHENGFENG
• LV ZHIHUA
• Wen Xuedan
• FAN HAO
• LU YUPENG

Assignees

• 大理大学

Dates

Publication Date

20260512

Application Date

20250619

Claims (9)

1. 1. A Mucor racemosus DLS157 is characterized in that the Mucor racemosus DLS157 is preserved in China general microbiological culture Collection center (CGMCC) in the 12 th month 30 of 2024, and the preservation number is CGMCC No.41738 and the preservation address is North Star XILU No. 1 and No. 3 in the Chaoyang area of Beijing city.
2. 2. Use of mucor circinelloides DLS157 according to claim 1 for the preparation of humic acid products from rice straw or bagasse as carbon source.
3. 3. A humic acid product, which is obtained by fermenting the Mucor racemosus DLS157 according to claim 1, wherein the carbon source used for the fermentation is rice straw or bagasse.
4. 4. A bacterial fertilizer characterized in that the bacterial fertilizer is prepared by fermenting straw powder and wheat bran by using the mucor circinelloides DLS157 according to claim 1.
5. 5. The use of the bacterial manure of claim 4 for promoting the growth of crops.
6. 6. The use according to claim 5, wherein the crop plants are wheat and chinese cabbage.
7. 7. The use according to claim 6, wherein when the crop is wheat, it has a promoting effect in terms of plant height, leaf length, leaf width and leaf number; when the crops are Chinese cabbage, the plant height, the leaf width, the leaf number, the fresh weight on the ground and the dry weight on the ground are promoted.
8. 8. The use of the bacterial manure of claim 4 for soil improvement.
9. 9. The use according to claim 8, characterized by the improvement that: (1) Increasing the content of humus in the soil; (2) Increasing the diversity and abundance of microbial colonies in the soil; (3) Regulating the structure of microbial colonies in the soil; (4) Promote the conversion and circulation of carbon, phosphorus and sulfur elements in soil.

Description

Mucor racemosus DLS157 and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a Mucor racemosus DLS157 and application thereof in soil improvement and crop growth promotion. Background Humus is a complex amorphous polymer colloid compound formed by long-term decomposition and transformation of dead animal and plant remains through microorganisms and accumulation of the remains after geophysical chemical action in the presence of moisture and air. In China, humic acid is classified into three types of humic acid, humin and humic acid according to its solubility and color in a solvent. The fulvic acid can be dissolved in acid, alkali and neutral medium, can promote the decomposition of minerals and the release of nutrients in soil, and has important roles in the accumulation and regeneration process of the humic acid. The humins have negative charges and are hydrophilic colloids, and can absorb or replace potassium, sodium, ammonium ions and the like in soil, so that soil particles become small reservoirs and fertilizer reservoirs for water and fertilizer retention, soil pores are increased, and soil fertility is improved. The humic acid is the most active part in the humus component, has higher cation exchange capacity, can improve the water and fertilizer retention capacity and adsorption capacity of soil, and is beneficial to promoting the formation of good structures of the soil. Therefore, the humic acid can improve soil, activate indissolvable mineral elements, promote the absorption of trace elements by plants, promote the slow release of nutrient elements, stimulate plant development and enhance plant resistance. Although the theory that humic acid originates from plants is recognized, it remains the focus of controversy as to which components in plants constitute humic acid. There are mainly four theories about the origin of humic acid, namely lignin-protein theory, polyphenol self-condensation, polyphenol-protein pathway and maillard reaction. There is also controversy about the specific role of microorganisms in the formation of humic acid, mainly the following four hypothesis plant transformation hypothesis biochemical hypothesis, cell autolysis, and microbial synthesis. It is currently difficult to determine which hypothesis is closer to the actual situation, and many processes may be required to cooperate to form humic acid. The hypothesis of microbial formation mainly emphasizes the role of microorganisms in the formation of humic acid. Microorganisms play a multi-directional role in the humic acid formation process, and not only act as decomposers to decompose macromolecular polymers, but also act as producers to recondense decomposed small molecular compounds. Therefore, the lignocellulose decomposition products are skeleton and substrate formed by humic acid. Adding organic waste rich in proteins at a suitable time, increasing the activity of the lignocellulose enzymes, by controlling key factors, provides a necessary choice for the conversion of lignocellulose waste to humic acid. The lignocellulose component is a richer component in agricultural wastes and mainly consists of three-dimensional network structures such as cellulose, hemicellulose, lignin and the like. Lignocellulose is used as renewable biological resource with the largest yield in the biological world, the annual yield of the lignocellulose reaches 1500 hundred million tons, and the yield of the straw reaches 60 hundred million tons. As a large agricultural country, the annual output of Chinese straw is 11.13 hundred million tons, which accounts for about one fifth of the global straw resource. Therefore, strains for efficiently producing humic acid by utilizing the agricultural straw are screened out, so that humic acid with high added value is produced, and the agricultural straw is recycled. The method has important significance in aspects of agricultural treatment, soil restoration, humic acid production by microbial fermentation and the like. Disclosure of Invention Aiming at the problems, the invention provides a Mucor racemosus DLS157 and application thereof in soil improvement and crop growth promotion. In order to achieve the above purpose, the present invention adopts the following technical scheme: The Cladosporium roseum DLS157 is classified and named as Cladosporium roseum Mucor racemosus, and is preserved in China general microbiological culture Collection center (CGMCC) No.41738 in the year 2024, 12 months 30, and the preservation address is No.1, 3 in the Guangdong region North Star West-Shang of Beijing city. It is another object of the present invention to provide the use of Mucor circinelloides DLS157 in the preparation of high value humic acid products. It is another object of the present invention to provide a high value humic acid product comprising a culture or processed product of Mucor racemosus DLS157 as described above. It is another