CN-120758641-B - Molecular marker and amplification primer pair related to chicken carcass traits and meat quality traits and application of molecular marker and amplification primer pair

CN120758641BCN 120758641 BCN120758641 BCN 120758641BCN-120758641-B

Abstract

The invention discloses a molecular marker and amplification primer pair related to chicken carcass traits and meat quality traits and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.3, SNP1 with C/T mutation at 112 th base, SNP2 with C/T mutation at 177 th base, SNP3 with A/C mutation at 194 th base, SNP4 with T/C mutation at 203 th base, SNP5 with A/G mutation at 442 th base and SNP6 with T/A mutation at 681 st base of the sequence shown as SEQ ID NO. 3. The invention discovers that a plurality of SNP loci on PPM1K gene are obviously related to chicken carcass traits and meat quality traits, provides a new SNP molecular marker for molecular marker assisted selection, and provides scientific basis for chicken breeding.

Inventors

NIE QINGHUA
CAI BAILIN
LI JIAHAO
GUO SUYIN
LUO XUEHUI
ZHENG XINYI
GUO SIYU
LI YAOHUA
SUN YU
ZHOU ZHEN

Assignees

华南农业大学
清远市清城区清远鸡研究院
清远市清城区动物卫生防疫中心

Dates

Publication Date: 20260508
Application Date: 20250716

Claims (5)

1. The application of molecular markers as targets in identifying chicken carcass traits and meat traits, which are characterized by comprising a loss rate of cooking, a wing weight, a shank length, a slaughter rate, a leg muscle weight, a leg muscle a value, a leg muscle rate, a chest width, a leg muscle b value, a shank circumference, an inter-muscular fat width, a leg muscle L value and a pectoral muscle b value; The molecular markers are a combination of SNP1, SNP2, SNP3, SNP4, SNP5 and SNP 6; The SNP1 is a C/T single nucleotide polymorphism at the 112 th position of a sequence shown as SEQ ID NO.3, the SNP2 is a C/T single nucleotide polymorphism at the 177 th position of the sequence shown as SEQ ID NO.3, the SNP3 is an A/C single nucleotide polymorphism at the 194 th position of the sequence shown as SEQ ID NO.3, the SNP4 is a T/C single nucleotide polymorphism at the 203 th position of the sequence shown as SEQ ID NO.3, the SNP5 is an A/G single nucleotide polymorphism at the 442 th position of the sequence shown as SEQ ID NO.3, and the SNP6 is a T/A single nucleotide polymorphism at the 681 th position of the sequence shown as SEQ ID NO. 3; at SNP1 locus, the steaming loss rate of CT genotype individuals is higher than TT genotype individuals; At SNP2 locus, the steaming loss rate of CT genotype individual is higher than TT genotype individual; In SNP3 locus, the wing weight and the shank length of CC genotype individual and AC genotype individual are lower than those of AA genotype individual, the leg muscle a value of CC genotype individual and AC genotype individual is higher than that of AA genotype individual, the slaughter rate and leg muscle weight of AA genotype individual are higher than those of CC genotype individual, the leg muscle rate of AA genotype individual is lower than that of CC genotype individual; at SNP4 locus, the chest width of CC genotype individual and TC genotype individual is higher than TT genotype individual, the leg muscle b value of TT genotype individual is higher than CC genotype individual; in SNP5 locus, GG genotype individual and AG genotype individual chest width and shin circumference are higher than AA genotype individual; at SNP6 site, the intramuscular fat width of AA genotype individual is higher than that of TT genotype individual, the intramuscular fat width of TA genotype individual is lower than that of TT genotype individual, the tibial circumference, leg muscle weight and chest width of AA genotype individual and TA genotype individual are higher than those of TT genotype individual, the pectoral muscle b value of AA genotype individual and TA genotype individual is lower than that of TT genotype individual, and the leg muscle L value of AA genotype individual is higher than that of TT genotype individual.
2. A method for identifying chicken carcass traits and meat quality traits, comprising the steps of: Taking genomic DNA of a chicken to be detected as a template, and carrying out PCR amplification by using a primer pair for detecting molecular markers related to carcass traits and meat quality traits of the chicken or a kit containing the primer pair to obtain an amplification product; The primer pair comprises an upstream primer with a sequence shown as SEQ ID NO.1 and a downstream primer with a sequence shown as SEQ ID NO. 2; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.3, SNP1 with C/T mutation at 112 th base, SNP2 with C/T mutation at 177 th base, SNP3 with A/C mutation at 194 th base, SNP4 with T/C mutation at 203 th base, SNP5 with A/G mutation at 442 th base and SNP6 with T/A mutation at 681 st base of the sequence shown as SEQ ID NO. 3; at SNP1 locus, the steaming loss rate of CT genotype individuals is higher than TT genotype individuals; At SNP2 locus, the steaming loss rate of CT genotype individual is higher than TT genotype individual; In SNP3 locus, the wing weight and the shank length of CC genotype individual and AC genotype individual are lower than those of AA genotype individual, the leg muscle a value of CC genotype individual and AC genotype individual is higher than that of AA genotype individual, the slaughter rate and leg muscle weight of AA genotype individual are higher than those of CC genotype individual, the leg muscle rate of AA genotype individual is lower than that of CC genotype individual; at SNP4 locus, the chest width of CC genotype individual and TC genotype individual is higher than TT genotype individual, the leg muscle b value of TT genotype individual is higher than CC genotype individual; in SNP5 locus, GG genotype individual and AG genotype individual chest width and shin circumference are higher than AA genotype individual; at SNP6 site, the intramuscular fat width of AA genotype individual is higher than that of TT genotype individual, the intramuscular fat width of TA genotype individual is lower than that of TT genotype individual, the tibial circumference, leg muscle weight and chest width of AA genotype individual and TA genotype individual are higher than those of TT genotype individual, the pectoral muscle b value of AA genotype individual and TA genotype individual is lower than that of TT genotype individual, and the leg muscle L value of AA genotype individual is higher than that of TT genotype individual.
3. The method according to claim 2, wherein the PCR amplification reaction system comprises 2. Mu.L of the template DNA, 2x RapidTaq Master Mix 15. Mu.L of the upstream primer, 1.2. Mu.L of the downstream primer, 1.2. Mu. L, ddH 2 O10.6. Mu.L of the downstream primer.
4. The method according to claim 2, wherein the PCR amplification is performed by a reaction procedure of 95℃pre-denaturation for 3min, 95℃denaturation for 15s,58℃annealing for 15s,72℃extension for 15s,34 cycles, 72℃final extension for 5min, and 4℃storage.
5. The application of the molecular marker serving as a target in chicken molecular marker assisted breeding is characterized in that the molecular marker is one or more of SNP1, SNP2, SNP3, SNP4, SNP5 and SNP 6; The SNP1 is a C/T single nucleotide polymorphism at the 112 th position of a sequence shown in SEQ ID NO.3, the SNP2 is a C/T single nucleotide polymorphism at the 177 th position of the sequence shown in SEQ ID NO.3, the SNP3 is an A/C single nucleotide polymorphism at the 194 th position of the sequence shown in SEQ ID NO.3, the SNP4 is a T/C single nucleotide polymorphism at the 203 th position of the sequence shown in SEQ ID NO.3, the SNP5 is an A/G single nucleotide polymorphism at the 442 th position of the sequence shown in SEQ ID NO.3, and the SNP6 is a T/A single nucleotide polymorphism at the 681 th position of the sequence shown in SEQ ID NO. 3; at SNP1 locus, the steaming loss rate of CT genotype individuals is higher than TT genotype individuals; At SNP2 locus, the steaming loss rate of CT genotype individual is higher than TT genotype individual; In SNP3 locus, the wing weight and the shank length of CC genotype individual and AC genotype individual are lower than those of AA genotype individual, the leg muscle a value of CC genotype individual and AC genotype individual is higher than that of AA genotype individual, the slaughter rate and leg muscle weight of AA genotype individual are higher than those of CC genotype individual, the leg muscle rate of AA genotype individual is lower than that of CC genotype individual; at SNP4 locus, the chest width of CC genotype individual and TC genotype individual is higher than TT genotype individual, the leg muscle b value of TT genotype individual is higher than CC genotype individual; in SNP5 locus, GG genotype individual and AG genotype individual chest width and shin circumference are higher than AA genotype individual; at SNP6 site, the intramuscular fat width of AA genotype individual is higher than that of TT genotype individual, the intramuscular fat width of TA genotype individual is lower than that of TT genotype individual, the tibial circumference, leg muscle weight and chest width of AA genotype individual and TA genotype individual are higher than those of TT genotype individual, the pectoral muscle b value of AA genotype individual and TA genotype individual is lower than that of TT genotype individual, and the leg muscle L value of AA genotype individual is higher than that of TT genotype individual.

Description

Molecular marker and amplification primer pair related to chicken carcass traits and meat quality traits and application of molecular marker and amplification primer pair Technical Field The invention relates to the technical field of biology, in particular to a molecular marker related to chicken carcass traits and meat quality traits, an amplification primer pair and application thereof. Background Due to the influence of diseases such as avian influenza, the cold fresh sale of poultry becomes the mainstream of the market. Based on the above background, carcass traits and meat quality traits of poultry become important factors affecting consumer consumption. If the carcass traits and meat quality traits of poultry can be effectively improved, the method is beneficial to promoting the further development of the chicken raising industry. Single nucleotide polymorphisms (Single Nucleotide Polymorphism, SNPs) are the diversity of DNA sequences within the genome that are initiated by single nucleotide insertions, deletions, inversions, and transformations, etc. As a common one of the animal's heritable variations, it is widely distributed in the animal genome, has stable genetic properties and is easy to detect. In animal production, SNP is used for Molecular Mark-assside Selection (MAS), so that the limitation of traditional breeding can be broken through, and the precision of seed Selection and the breeding result of target characters can be improved. The Mg 2+/Mn2+ -dependent protein phosphatase 1K gene (protein phosphatase, mg 2+/Mn2+ dependent 1K, PPM 1K) is involved in the protein dephosphorylation process, and the encoded PPM1K protein plays an important role in regulating mitochondrial function. Mitochondria are important for muscle and skeletal development. However, no report exists on whether the PPM1K gene of the poultry is related to the carcass traits and the meat quality traits of the chickens. Disclosure of Invention The invention aims to provide a molecular marker related to chicken carcass traits and meat quality traits, an amplification primer pair and application thereof, so as to solve the problems in the prior art. The invention discovers that a plurality of SNP loci exist on the PPM1K gene, which are obviously related to chicken carcass traits and meat quality traits, and molecular markers are developed based on the SNP loci. The molecular marker provided by the invention can accurately identify the carcass traits and meat quality traits of chickens, thereby providing scientific basis for chicken breeding. In order to achieve the above object, the present invention provides the following solutions: the invention provides a molecular marker related to chicken carcass traits and meat quality traits, wherein the nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3; SNP1 with C/T mutation at base 112, SNP2 with C/T mutation at base 177, SNP3 with A/C mutation at base 194, SNP4 with T/C mutation at base 203, SNP5 with A/G mutation at base 442, and SNP6 with T/A mutation at base 681 of the sequence shown in SEQ ID NO. 3. The invention also provides a primer pair for amplifying the molecular marker, which comprises an upstream primer with a sequence shown as SEQ ID NO.1 and a downstream primer with a sequence shown as SEQ ID NO. 2. The invention also provides a kit for identifying chicken carcass traits and meat quality traits, wherein the kit comprises the primer pair. The invention also provides an application of the primer pair or the kit in identifying chicken carcass traits and meat quality traits, wherein the chicken carcass traits and meat quality traits comprise a boiling loss rate, a wing weight, a shank length, a slaughter rate, a leg muscle weight, a leg muscle a value, a leg muscle rate, a chest width, a leg muscle b value, a shin circumference, an intermuscular fat width, a leg muscle L value and a pectoral muscle b value. The invention also provides a method for identifying chicken carcass traits and meat quality traits, which comprises the following steps: taking chicken genome DNA to be detected as a template, and carrying out PCR amplification by using the primer pair or the kit to obtain an amplification product; at SNP1 locus, the steaming loss rate of CT genotype individuals is higher than TT genotype individuals; At SNP2 locus, the steaming loss rate of CT genotype individual is higher than TT genotype individual; In SNP3 locus, the wing weight and the shank length of CC genotype individual and AC genotype individual are lower than those of AA genotype individual, the leg muscle a value of CC genotype individual and AC genotype individual is higher than that of AA genotype individual, the slaughter rate and leg muscle weight of AA genotype individual are higher than those of CC genotype individual, the leg muscle rate of AA genotype individual is lower than that of CC genotype individual; at SNP4 locus, the chest width of CC genotype individual and TC genotyp