CN-120796041-B - Circulation micro-fluidic device for cell sorting and application method thereof

Abstract

The invention discloses a circulating micro-flow device for cell sorting and a use method thereof, which belong to the technical field of cell sorting, and the circulating micro-flow device comprises a liquid storage tank, a filter screen plate, a reciprocating screw rod, a movable frame, a cleaning plate and a compression spring, wherein after positioning slots on two sides of the filter screen plate are correspondingly clamped on the surfaces of positioning clamping strips on the inner wall of the liquid storage tank, the filter screen plate is tightly adhered to the cleaning plate, the cleaning plate is jacked up, so that the compression spring is extruded and deformed between the movable frame and the cleaning plate, the cleaning plate is pressed on the surface of the filter screen plate under the rebound action of the pressing spring, a proper amount of cell stock solution is poured into the opening of the liquid storage tank, and meanwhile, the rotating motor is started to control the reciprocating screw rod to rotate, so that the movable frame moves reciprocally on the surface of the reciprocating screw rod, the cleaning plate is driven to move back and forth on the surface of the filter screen plate, cell fragments accumulated on the surface of the filter screen plate are cleaned, the situation that the cell fragments are accumulated on the surface of the filter screen plate for a long time to block the filter screen plate is avoided, and the filtering effect on the cell stock solution is affected.

Inventors

• LIU JIANTUO
• LI HONGRUI
• HE YANLI
• ZHENG JINE
• HUANG SHIANG

Assignees

• 武汉康圣达医学检验所有限公司

Dates

Publication Date

20260505

Application Date

20250721

Claims (4)

1. 1. The circulating micro-flow device for cell sorting comprises a concave base (1) and is characterized in that an installation base plate (2) is fixedly installed at the bottom of one side, far away from an opening, of the concave base (1), T-shaped clamping grooves (201) are symmetrically formed in the top of the installation base plate (2), a treatment box (3) is arranged at the top of the installation base plate (2), T-shaped clamping strips (301) are symmetrically and fixedly installed at the bottom of the treatment box (3), the T-shaped clamping strips (301) penetrate through the inside of the T-shaped clamping grooves (201), a liquid storage box (4) is fixedly installed at the top of the concave base (1), clamping mechanisms (5) are fixedly installed at positions, close to the treatment box (3), of the tops of two sides of the liquid storage box (4), cleaning mechanisms (6) are arranged in the liquid storage box (4), and filter screen plates (7) are installed in the liquid storage box (4) in a penetrating and sliding mode on one side of the cleaning mechanisms (6). The bottom of the arc surface of the treatment box (3) is provided with a liquid outlet (302), a blocking block (303) is clamped in the liquid outlet (302), fixed clamping blocks (304) are fixedly arranged at the tops of the two side surfaces of the treatment box (3) respectively, a magnetic attraction cover plate I (305) is rotatably arranged at one side of the top of the treatment box (3) through a pin shaft, a spiral flow channel (306) is fixedly arranged on the inner wall of the treatment box (3), and a plurality of separation barrier strips (307) are fixedly arranged at the bottom in a track of the spiral flow channel (306) at equal intervals; The bottom end of the spiral flow channel (306) is fixedly provided with a flow distribution frame (308), one end, far away from the spiral flow channel (306), of the flow distribution frame (308) penetrates through the treatment box (3) and corresponds to the rectangular through groove (103), two sides of the flow distribution frame (308) are respectively fixedly provided with a first liquid discharge frame (309) and a second liquid discharge frame (310), the first liquid discharge frame (309) and the second liquid discharge frame (310) are respectively communicated with two sides of the flow distribution frame (308), and one ends, far away from the flow distribution frame (308), of the first liquid discharge frame (309) and the second liquid discharge frame (310) penetrate through the treatment box (3) and are respectively fixedly connected with the treatment box (3); The clamping mechanism (5) comprises a concave mounting frame (501), a fixed clamping plate (502) is rotatably mounted on one side of the concave mounting frame (501) through a pin shaft, the position of a bayonet of the fixed clamping plate (502) corresponds to that of a fixed clamping block (304), positioning convex blocks (503) are symmetrically and fixedly mounted on one sides, far away from the bayonet of the fixed clamping plate (502), of the concave mounting frame (501) and the fixed clamping plate (502), the positioning convex blocks (503) on the surfaces of the concave mounting frame (501) and the fixed clamping plate (502) are opposite to each other, positioning sleeves (504) are fixedly mounted on the surfaces, located on one side of the positioning convex blocks (503), of the concave mounting frame (501) and the fixed clamping plate (502), a reset spring (505) is sleeved between the two opposite positioning convex blocks (503), and two ends of the reset spring (505) are clamped inside the positioning sleeves (504); The cleaning mechanism (6) comprises a reciprocating screw rod (601), two ends of the reciprocating screw rod (601) are rotatably connected to the inner wall of the liquid storage tank (402) through bearings, one end of the reciprocating screw rod (601) penetrates through the liquid storage tank (4) and is fixedly provided with a first bevel gear (602), the surface of the first bevel gear (602) is meshed with a second bevel gear (603), one side of the second bevel gear (603) is fixedly provided with a rotating motor (604), the rotating motor (604) is fixedly connected to the outer surface of the liquid storage tank (4), and the surface of the reciprocating screw rod (601) is in threaded connection with a movable frame (605); The utility model discloses a liquid storage tank, including movable frame (605), fixed mounting, limiting rod (610), reservoir (402) inner wall fixed connection, movable frame (605) both sides run through slidable mounting respectively has compressing tightly pole (606), one end of compressing tightly pole (606) is passed movable frame (605) fixed mounting and is had limiting plate (607), two fixed mounting has clearance board (608) between the one end that limiting plate (607) was kept away from to compressing tightly pole (606), compressing tightly pole (606) are located surface between movable frame (605) and clearance board (608) and have cup jointed compression spring (609), movable frame (605) both ends run through slidable mounting respectively and have limiting slide bar (610), and limiting slide bar (610) both ends and reservoir (402) inner wall fixed connection.
2. 2. The circulating microfluidic device for cell sorting according to claim 1, wherein a collection box (101) is installed at an opening on one side of the concave base (1) in a penetrating and sliding manner, rubber strips (102) are symmetrically and fixedly installed on the surfaces on two sides of the collection box (101), and a rectangular through groove (103) is formed in one side, far away from the opening, of the concave base (1).
3. 3. The circulating micro-fluidic device for cell sorting according to claim 1, wherein a magnetic attraction cover plate II (401) is rotatably arranged on one side of the top of the liquid storage tank (4) through a pin shaft, a liquid storage tank (402) is arranged in the liquid storage tank (4), positioning clamping strips (403) are symmetrically and fixedly arranged on the inner wall of one side of the opening of the liquid storage tank (402), positioning slots (701) are respectively arranged on two sides of the filter screen plate (7), the positioning slots (701) penetrate through the surfaces of the positioning clamping strips (403), a liquid discharge groove (404) is arranged on one side, far away from the opening of the liquid storage tank (402), of the top of the liquid storage tank (4), the liquid discharge groove (404) corresponds to the position of the opening of the top of the spiral flow channel (306), a circulating pump (405) is fixedly arranged on one side of the liquid discharge groove (404) in an embedded mode, the input end and the output end of the circulating pump (405) are respectively communicated with a guide pipe, and the guide pipe is respectively communicated with the liquid storage tank (402) and the liquid discharge groove (404).
4. 4. A method of using a circulating microfluidic device for cell sorting according to any one of claims 1-3, characterized in that the method of use comprises the steps of: Selecting a treatment box (3) provided with a proper spiral flow channel (306) according to cell sorting requirements, taking the bottom of the treatment box (3) at the bottom of a mounting bottom plate (2), enabling a T-shaped clamping strip (301) at the bottom of the treatment box (3) to correspond to a T-shaped clamping groove (201), pushing the treatment box (3) to move at the top of the mounting bottom plate (2) until the T-shaped clamping strip (301) is inserted into the corresponding T-shaped clamping groove (201), enabling the treatment box (3) to be gradually tightly attached to one side of a concave base (1) and a liquid storage box (4), enabling the top end of the spiral flow channel (306) to be tightly attached to the inside of a liquid discharge groove (404), enabling a shunt frame (308) to be inserted into the inside of a rectangular through groove (103), enabling a rubber strip (102) to be extruded and deformed at two sides of the collection box (101), enabling the collection box (101) to be fixedly clamped in the inside of the concave base (1), enabling a fixing clamping block (304) to be gradually attached to one side of the liquid storage box (4), enabling a fixing block (502) to be tightly attached to one side of the corresponding T-shaped clamping groove (502), enabling a fixing block (502) to be tightly attached to the fixing block (502) to be rotatably fixed between the fixing blocks (502) and a clamping block (502) to be rotationally deformed until the fixing block (502) is fixedly bent between the fixing block and a clamping block (502) is pressed, the fixed clamping plate (502) is jacked up to reset under the rebound action of the reset spring (505), and the bayonet of the fixed clamping plate (502) is clamped with the fixed clamping block (304), so that the treatment box (3) is clamped and fixed on one side of the liquid storage box (4); The second magnetic suction cover plate (401) is turned over to open the positioning slots (701) on the two sides of the filter screen plate (7) and the positioning clamping strips (403) on the inner wall of the liquid storage tank (402) to correspond to each other, the filter screen plate (7) is pushed to be inserted into the liquid storage tank (402), the positioning slots (701) are clamped on the surfaces of the corresponding positioning clamping strips (403) to slide, the filter screen plate (7) and the cleaning plate (608) are tightly adhered to each other, the filter screen plate (7) jacks up the cleaning plate (608), the cleaning plate (608) is enabled to be extruded and deformed between the movable frame (605) and the cleaning plate (608), the cleaning plate (608) is pressed on the surface of the filter screen plate (7) under the rebound effect of the pressing spring (609), a proper amount of cell stock solution is poured into one side of the filter screen plate (7) inside the liquid storage tank (402), the cell stock solution flows into a guide pipe in the liquid storage tank (402) through the input end of the filter screen plate (7), the second magnetic suction cover plate (401) is closed on the top of the liquid storage tank (4), the circulating pump (405) is started to enable the cell stock solution to flow into the spiral liquid drain (306) from the liquid storage tank (402) to the inner side of the liquid storage tank (306) through the guide pipe, the method comprises the steps that when cell stock solutions of different sizes flow, the cell stock solutions are separated and blocked by a separation barrier (307), so that the cell stock solutions of different sizes flow to two sides of the separation barrier (307) to be separated until the separated cell stock solutions and cells flow to a flow dividing frame (308) from a spiral runner (306), and the cell stock solutions of different sizes flow to a collecting box (101) in a concave base (1) from the flow dividing frame (308) to be discharged from the first liquid discharging frame (309) and the second liquid discharging frame (310) after the separation of the flow dividing frame (308), and the surplus cell stock solutions flow to the collecting box (101) in the concave base (1) to be concentrated until the separation of the cell stock solutions in a liquid storage tank (402) is completed; Simultaneously, cell fragments in the cell stock solution are filtered by the filter screen plate (7), so that the cell fragments are accumulated on one side of the filter screen plate (7), the rotating motor (604) is started to control the reciprocating screw rod (601) to synchronously rotate under the meshing action of the first bevel gear (602) and the second bevel gear (603), the movable frame (605) moves back and forth on the surface of the reciprocating screw rod (601), meanwhile, two ends of the movable frame (605) slide on the surface of the corresponding limit sliding rod (610), the movable frame (605) is ensured not to incline and deflect in the moving process, the cleaning plate (608) moves on one side of the filter screen plate (7) along with the movable frame (605), and the cell fragments accumulated on the surface of the filter screen plate (7) are cleaned, so that the influence of the cell fragments accumulated on the surface of the filter screen plate (7) on the filtering effect of the cell stock solution is avoided.

Description

Circulation micro-fluidic device for cell sorting and application method thereof Technical Field The invention relates to the technical field of cell sorting, in particular to a circulating micro-fluidic device for cell sorting and a use method thereof. Background Cell sorting plays a vital role in a plurality of fields such as biology, medical research, clinical diagnosis and treatment, and the like, accurately and efficiently separates specific types of cells from complex cell mixtures, and has irreplaceable significance for deep understanding of cell functions, disease occurrence mechanisms and development of targeted treatment methods, wherein a microfluidic device is used as important equipment for cell sorting, and by designing a specific microchannel structure and a fluid driving mode, accurate control and sorting of cells are realized, so that cell stock flows in a flow channel, and cells or particles with different sizes are separated under the action of inertial force when the cell stock flows, so that the cells in the cell stock are sorted according to the cell size. When the cell stock solution is subjected to cell sorting by the conventional circulating microfluidic device, the cell stock solution contains a large amount of cell fragments, and when the cell stock solution is subjected to primary filtration, the cell fragments are easily accumulated to block the filter screen plate, so that the filter effect of the filter screen plate on the cell stock solution is reduced, the effect of cell sorting is affected, and meanwhile, as the cell sorting device is generally taken as a whole, different flow channels are difficult to replace at will according to different cell sorting requirements, and therefore, improvement is needed. Based on the above, the present invention is to design a circulating microfluidic device for cell sorting and a method of using the same, so as to solve the above-mentioned problems. Disclosure of Invention The invention aims to provide a circulating micro-flow device for cell sorting and a use method thereof, which are used for solving the problems that when cell sorting is carried out on cell stock solution by the conventional circulating micro-flow device for cell sorting, as the cell stock solution contains a large amount of cell fragments, when primary filtration is carried out, the cell fragments are easy to accumulate and block a filter screen plate, so that the filtering effect of the filter screen plate on the cell stock solution is reduced, the effect of cell sorting is influenced, and meanwhile, as the cell sorting device is generally taken as a whole, different flow channels are difficult to be replaced at will according to different cell sorting requirements. In order to achieve the above purpose, the present invention provides the following technical solutions: The utility model provides a circulation microfluidic device for cell sorting, includes concave base, the bottom fixed mounting that opening one side was kept away from to concave base has mounting plate, mounting plate top symmetry is equipped with T type draw-in groove, the mounting plate top is equipped with handles the case, handle case bottom symmetry fixed mounting and have T type card strip, and T type card strip runs through sliding connection in T type draw-in groove inside, concave base top fixed mounting has the liquid reserve tank, the top of liquid reserve tank both sides is close to the position of managing the case fixed mounting respectively has joint mechanism, the inside clearance mechanism that is equipped with of liquid reserve tank, the inside clearance mechanism that is located one side of liquid reserve tank runs through sliding mounting has the filter screen plate. As a further scheme of the invention, the opening at one side of the concave base is penetrated and slidably provided with the collecting box, the surfaces at two sides of the collecting box are respectively and symmetrically and fixedly provided with the rubber strips, and one side of the concave base far away from the opening is provided with the rectangular through groove. As a further scheme of the invention, a liquid outlet is arranged at the bottom of the arc surface of the treatment box, a blocking block is clamped in the liquid outlet, fixed clamping blocks are respectively and fixedly arranged at the tops of the two side surfaces of the treatment box, a first magnetic attraction cover plate is rotatably arranged at one side of the top of the treatment box through a pin shaft, a spiral runner is fixedly arranged on the inner wall of the treatment box, and a plurality of separation blocking bars are fixedly arranged at the bottom of the track of the spiral runner at equal intervals. As a further scheme of the invention, the bottom end of the spiral flow channel is fixedly provided with a flow dividing frame, one end of the flow dividing frame, which is far away from the spiral flow channel, penetrates through th