CN-120801700-B - Protein imprinting processing device

Abstract

The invention belongs to the technical field of protein antibody incubation, and provides a protein blotting treatment device which comprises a base, a limit column fixedly arranged on the base, a cam part arranged at one end of the limit column, a reaction groove movably arranged on the upper side of the second fixed disk and penetrating through the second fixed disk to be movably connected with the other end of the first push rod, wherein the cam part is arranged between the first fixed disk and the second fixed disk and is provided with a preset distance from the second fixed disk. Through adopting above-mentioned structure, can realize the swing of reaction tank simultaneously with the help of cam component and first push rod when the carousel rotates.

Inventors

• MENG CHENGWEI
• YANG ZHOU

Assignees

• 上海天能生命科学有限公司

Dates

Publication Date

20260505

Application Date

20250910

Claims (20)

1. 1. A protein blotting processing device, comprising: A base; The limiting column is fixedly arranged on the base at one end, the limiting column is provided with a preset length, a cam component which is fixedly arranged is arranged at one end of the limiting column, which is far away from the base, and at least one concave and/or one convex part is arranged on the cam component; The rotary table is arranged in a rotary way relative to the limit column and comprises a first fixed disc and a second fixed disc which are connected with each other, the first fixed disc is arranged close to one side of the base, the second fixed disc is arranged close to one side of the cam part, the cam part is positioned between the first fixed disc and the second fixed disc, a preset interval is arranged between the cam part and the second fixed disc, The first push rod is movably arranged on the lower side surface of the second fixed disc, one end of the first push rod is in contact with the cam component, and the first push rod can reciprocate along the axis direction of the first push rod; The reaction tank is movably arranged on the upper side surface of the second fixed disc, a first connecting part is arranged on the reaction tank, a first through notch is arranged on the second fixed disc, the first connecting part penetrates through the first notch and is movably connected with the other end of the first push rod, and the first push rod can drive the reaction tank to swing back and forth relative to the second fixed disc.
2. 2. The protein blotting processing device according to claim 1, wherein a first roller is provided at a contact end of the first push rod and the cam member, the first roller is rotatably provided on the first push rod, and the first roller is abutted to the cam member.
3. 3. The protein blotting processing device according to claim 1, wherein the concave portion and the convex portion are of an arc surface structure, and the convex portion and the concave portion are connected in a smooth transition mode.
4. 4. The protein blotting processing device according to claim 1, wherein a first chute is arranged on the lower side surface of the second fixing plate, the first push rod movably penetrates through the first chute, a plurality of second rollers are arranged on two sides of the first chute, and the second rollers are in butt joint with the side surfaces of the first push rod.
5. 5. The device of claim 4, further comprising a first return spring, wherein one end of the first return spring is connected to the outside of the first chute, and the other end of the first return spring is connected to the first push rod.
6. 6. The western blot processing apparatus of claim 1, wherein an adapted incubation box is provided in the reaction tank, and wherein the incubation box is detachably provided on the reaction tank.
7. 7. The protein blotting processing device according to claim 6, further comprising a third fixed disk rotatably disposed with respect to the limit post, wherein the third fixed disk is disposed between the first fixed disk and the second fixed disk, and the third fixed disk is connected to the first fixed disk and the second fixed disk, respectively; The device comprises a first fixing disc, a second fixing disc, a third fixing disc, a plurality of test tube assemblies, a plurality of first mounting holes, a first adapter and a first refrigerating sheet, wherein the second fixing disc and the third fixing disc are provided with the test tube assemblies, the test tube assemblies comprise a first support arranged on the second fixing disc and a heat preservation cavity arranged on the third fixing disc, the first support is provided with the first mounting holes, the first mounting holes are communicated with the heat preservation cavity, the heat preservation cavity is internally provided with the first adapter and the first refrigerating sheet, the first adapter is used for supporting the bottom of a test tube, and the first refrigerating sheet is used for refrigerating in the heat preservation cavity.
8. 8. The protein blotting processing device of claim 7, wherein a first radiator and a temperature monitoring sensor are further arranged on the outer side of the heat preservation cavity, the first radiator comprises a plurality of first radiating fins which are arranged at intervals, and the temperature monitoring sensor is used for detecting the temperature in the heat preservation cavity.
9. 9. The western blot processing apparatus according to claim 7, wherein a cleaning tank is further provided on the first support, the cleaning tank includes a first cleaning hole and a second cleaning hole, the inner diameter of the first cleaning hole is larger than the inner diameter of the second cleaning hole, and the depth of the first cleaning hole is larger than the depth of the second cleaning hole.
10. 10. The device of claim 9, wherein the central axes of the plurality of first mounting holes, the first washing holes and the second washing holes are all located on the same circumferential line.
11. 11. The apparatus according to claim 10, wherein the first support and the reaction tank are alternately disposed on the second fixed plate.
12. 12. The protein blotting processing device as defined in claim 11, further comprising a second refrigerating plate and a refrigerating seat, wherein the second refrigerating plate is fixedly arranged relative to the third fixed plate, and the second refrigerating plate is connected with the refrigerating seat; the reaction tank is provided with a second notch, a refrigeration contact seat is arranged at the second notch, the refrigeration contact seat is in contact with the incubation box, and one end of the refrigeration seat is in butt joint with the refrigeration contact seat.
13. 13. The protein blotting treatment device according to claim 12, wherein a smooth guiding structure is arranged between the refrigeration contact seat and the refrigeration seat, the smooth guiding structure comprises a first arc-shaped groove arranged on the refrigeration contact seat and a first arc-shaped boss arranged at one end of the refrigeration seat, the first arc-shaped groove is adapted to the first arc-shaped boss, and a rotation axis of the refrigeration contact seat relative to the second fixed disc coincides with a circle center of the first arc-shaped groove.
14. 14. The protein blotting processing device according to claim 13, wherein a first heat insulation seat is further arranged on the third fixing plate, a through first refrigeration channel is arranged on the first heat insulation seat, the refrigeration seat penetrates through the first refrigeration channel to be in butt joint with the refrigeration contact seat, a first yielding groove is arranged on the first heat insulation seat, the position of the first yielding groove corresponds to the position of the first connecting portion, and the first push rod can extend into the first yielding groove to be connected with the first connecting portion.
15. 15. The protein blotting processing device of claim 14, wherein the first heat insulating base is provided with a second radiator, the second radiator comprises a plurality of second radiating fins arranged in parallel, and the second radiating fins are located on the lower side of the third fixing plate.
16. 16. The device according to any one of claims 1 to 15, further comprising a lifting pipetting needle, wherein the pipetting needle is provided with a first pipetting channel, one end of the pipetting needle is provided with a first fixing block, a first sub-channel, a second sub-channel and a third sub-channel are arranged on the first fixing block, and the first sub-channel, the second sub-channel and the third sub-channel are all communicated with the first pipetting channel.
17. 17. The western blot processing apparatus of claim 16, further comprising a waste pump, a pure water pump, and a closed liquid pump disposed on the base, wherein the waste pump is in communication with the first sub-channel through a first valve, the pure water pump is in communication with the second sub-channel through a second valve, and the closed liquid pump is in communication with the third sub-channel through a third valve.
18. 18. The protein blotting processing apparatus of claim 17, wherein a plunger pump is disposed between said second valve and said second sub-channel, said plunger pump being in series with said second valve.
19. 19. The device of claim 16, further comprising a first connecting tube, a first lifting block and a second lifting block, wherein the second lifting block is connected with the first lifting block, a preset distance is arranged between the second lifting block and the first lifting block, one end of the first connecting tube is connected with the first fixed block, the other end of the first connecting tube is connected with the first lifting block, the first lifting block is arranged on the first lifting block, the first lifting block can be separated from the first lifting block, the moving directions of the first lifting block, the first lifting block and the second lifting block are identical, and the central axis of the first connecting tube is parallel to the central axis of the liquid suction needle.
20. 20. The protein blotting processing device of claim 19, further comprising a first connecting rod and a first guide rod, wherein the first connecting rod and the first guide rod are arranged in parallel, a first guide sleeve is arranged on the first guide rod, the first guide sleeve is connected with the first lifting block, the first lifting block movably penetrates through the first guide rod, and the first connecting rod penetrates through the first lifting block and is connected with the first lifting block.

Description

Protein imprinting processing device Technical Field The invention belongs to the field of protein antibody incubation, and particularly relates to a protein imprinting treatment device. Background Western blot (Western blotting) is an experimental technique for detecting and analyzing specific proteins, and is widely used in biomedical research. Separating protein in a sample by polyacrylamide gel electrophoresis (PAGE), separating according to the molecular weight of the protein, transferring the separated protein to a solid support (such as PVDF or nitrocellulose membrane) to fix the protein on the membrane, and then blocking non-specific binding sites on the membrane by protein without target protein (such as bovine serum albumin BSA or skimmed milk powder), thereby reducing background noise. The membrane is first incubated with a specific primary antibody, binding to the target protein, and then the primary antibody (usually labeled with an enzyme or fluorescent dye) is used to detect the target protein bound by the primary antibody. The bound secondary antibody is detected by chemiluminescence, fluorescence or colorimetry, thereby recognizing the target protein and analyzing the expression level thereof. Most of the existing western blot processing devices are semi-automatic processing structures, meanwhile, all parts of the existing devices are mutually independent, driving devices are more, structural equipment is complex, the size is large, in addition, participation of partial processes is needed manually, and full-automatic operation cannot be really achieved. Disclosure of Invention The invention provides a protein imprinting processing device, which solves the problems that most of the prior devices in the background technology are semi-automatic processing structures, and cannot realize real full-automatic processing, and meanwhile, the prior devices have individual modules, more driving devices, large size of the whole device and the like. In order to achieve the above purpose, the invention adopts the following technical scheme: A protein blotting processing device comprising: A base; The limiting column is fixedly arranged on the base at one end, the limiting column is provided with a preset length, a cam component which is fixedly arranged is arranged at one end of the limiting column, which is far away from the base, and at least one concave and/or one convex part is arranged on the cam component; The rotary table is arranged in a rotary way relative to the limit column and comprises a first fixed disc and a second fixed disc, the first fixed disc is arranged close to one side of the base, the second fixed disc is arranged close to one side of the cam part, the cam part is positioned between the first fixed disc and the second fixed disc, the cam part and the second fixed disc are provided with preset intervals, The first push rod is movably arranged on the lower side surface of the second fixed disc, one end of the first push rod can be abutted against the cam component, and the first push rod can reciprocate along the axis direction of the first push rod; The reaction tank is movably arranged on the upper side surface of the second fixed disc, a first connecting part is arranged on the reaction tank, a first through notch is arranged on the second fixed disc, the first connecting part penetrates through the first notch and is movably connected with the other end of the first push rod, and the first push rod can drive the reaction tank to swing back and forth relative to the second fixed disc. In some embodiments, a contact end of the first push rod and the cam component is provided with a first roller, the first roller is rotatably arranged on the first push rod, and the first roller can be abutted with the cam component. In some embodiments, the recess and the projection are both arcuate structures, and the projection and the recess are joined by a smooth transition. In some embodiments, a first sliding groove is formed in the lower side face of the second fixing disc, the first push rod movably penetrates through the first sliding groove, a plurality of second rollers are arranged on two sides of the first sliding groove, and the second rollers are in butt joint with the side face of the first push rod. In some embodiments, the sliding device further comprises a first return spring, one end of the first return spring is connected to the outer side of the first sliding groove, and the other end of the first return spring is connected to the first push rod. In some embodiments, an adapted incubation box is provided in the reaction tank, and the incubation box is detachably provided on the reaction tank. In some embodiments, the device further comprises a third fixed disk disposed between the first fixed disk and the second fixed disk, the third fixed disk being connected to the first fixed disk and the second fixed disk, respectively; The device comprises a first fixing disc, a second f