CN-120843536-B - Use of autophagy activators for increasing resistance to citrus canker

Abstract

The invention discloses application of a plant autophagy activator in improving citrus canker resistance, and relates to the technical field of biology. The invention discloses a forward regulation effect of autophagy gene-mediated autophagy pathway in citrus canker resistance, and applies an autophagy activator to improve citrus canker resistance for the first time. Through researches, the plant autophagy activator can effectively activate autophagy paths in citrus, so that the resistance of the plant autophagy activator to canker is remarkably enhanced. The discovery provides a solid theoretical basis and a practical basis for developing novel and environment-friendly plant disease resistance regulation and control technology. The plant autophagy activator has the advantages of wide sources, environmental friendliness, high safety and the like, does not negatively affect plant growth, does not bring potential risks to human, livestock and ecological environment, and meets the requirements of green agriculture and sustainable development.

Inventors

• WANG PENGWEI
• XIAO CUI
• HU RAN

Assignees

• 华中农业大学
• 湖北省农业科学院果树茶叶研究所

Dates

Publication Date

20260508

Application Date

20250711

Claims (2)

1. 1. A FhATG gene for regulating and controlling the resistance of citrus to canker is characterized in that the nucleotide sequence of the FhATG gene is shown as SEQ ID NO.2, and the regulation of the resistance of citrus to canker is to improve the sensitivity of citrus to canker by inhibiting the expression of the FhATG gene.
2. 2. Use of FhATG gene according to claim 1 for modulating canker resistance in citrus, wherein said use is to increase the sensitivity of citrus to canker by inhibiting expression of said FhATG gene.

Description

Use of autophagy activators for increasing resistance to citrus canker Technical Field The invention relates to the field of biotechnology, in particular to application of a plant autophagy activator in improving citrus canker resistance. Background Citrus canker is one of the bacterial diseases seriously damaging the citrus industry, and seriously affecting the appearance quality, yield and commodity value of fruits. To date, the prevention and treatment of citrus canker mainly depend on chemical pesticides such as copper preparations, but the long-term use of the chemical pesticides can easily cause the drug resistance of pathogenic bacteria and environmental pollution, and the chemical pesticides do not accord with the green development concept of the citrus industry. Therefore, development of a novel prevention and control medicament with low residue, high efficiency and safety is an urgent need for industrial sustainable development. Autophagy is a basic metabolic mechanism widely existing in eukaryotes, and has a core function of separating damaged components and metabolites through a membrane structure and then transferring the separated components and metabolites to vacuoles so as to decompose and reuse substances. Autophagy plays an important role in plant protection against pathogenic bacteria as a conserved cell cycle mechanism in eukaryotes. Early studies demonstrated that autophagy was involved in the defense response of arabidopsis gray mold and citrus yellow dragon disease. However, the current molecular mechanism of autophagy regulation against citrus canker is still unknown, and especially the strategy for realizing disease prevention and control by activating autophagy pathway through exogenous inducer is lack of theoretical basis and practical basis. Disclosure of Invention The object of the present invention is to provide the use of an autophagy activator for increasing citrus canker resistance, solving the problems of the prior art described above. According to the research, the autophagy activator can enhance the citrus canker resistance by promoting autophagy, so that important technical support is provided for preventing and treating the citrus canker. In order to achieve the above object, the present invention provides the following solutions: the invention provides the use of an autophagy activator for increasing citrus canker resistance. The invention also provides application of the plant autophagy activator in preparation of a preparation for improving citrus canker resistance. Further, the autophagy activator increases citrus canker resistance by increasing the expression level of autophagy genes. Further, the autophagy genes include FhATG gene, fhATG7 gene, fhATG a gene, and FhATG13 gene; Nucleotide sequences of the FhATG gene, the FhATG7 gene, the FhATG A gene and the FhATG gene are respectively shown as SEQ ID NO. 1-4. The invention also provides FhATG gene for regulating and controlling resistance of citrus to canker, and the nucleotide sequence of FhATG gene is shown as SEQ ID NO. 2. The invention also provides an application of FhATG gene in regulating and controlling resistance of citrus to canker. The invention also provides a method for controlling citrus canker, which comprises the step of activating autophagy paths of citrus plants to improve the resistance of citrus canker. Further, the autophagy pathway is activated by applying an autophagy activator to the citrus plant. The invention discloses the following technical effects: The invention discloses a forward regulation effect of autophagy gene-mediated autophagy pathway in citrus canker resistance, and applies an autophagy activator to improve citrus canker resistance for the first time. Through researches, the plant autophagy activator can effectively activate autophagy paths in citrus, so that the resistance of the plant autophagy activator to canker is remarkably enhanced. The discovery provides a solid theoretical basis and a practical basis for developing novel and environment-friendly plant disease resistance regulation and control technology. The invention takes the compound naturally existing in the plant body as the plant autophagy activator to induce the autophagy process in the plant cells, thereby activating the immune mechanism of the plant and realizing the effective prevention and control of the canker. Compared with the traditional chemical pesticide, the plant autophagy activator is derived from natural plants, has the advantages of wide sources, environmental friendliness, high safety and the like, does not negatively affect plant growth, does not bring potential risks to human, livestock and ecological environment, and meets the requirements of green agriculture and sustainable development. In addition, the application of the autophagy activator expands the new application of the autophagy activator in the field of plant pathology prevention and control, and opens up a brand new technical direc