CN-120866083-B - Endophytic fungus with functions of improving reversibility and promoting growth of water yeast Liu Kang and application thereof

Abstract

The invention discloses endophytic fungi with functions of improving the reversibility and promoting growth of water yeast Liu Kang and application thereof, belonging to the technical field of microorganisms, wherein the endophytic fungi is named Alternaria minutissima (ALTERNARIA TENUISSIMA) SLR21 and is preserved in China general microbiological culture collection center (CGMCC) No.42057. The invention screens endophytic fungi with the functions of improving the reversibility and promoting growth of the aquatic yeast Liu Kang by taking the aquatic yeast willow as a material, provides a new microorganism resource for digging new strains with biological energy functions, and provides a new path for solving the problem that the aquatic yeast Liu Chenglin is difficult. The fermentation liquor of the endophytic fungi contains substances such as polyphenol, polysaccharide, flavone, salicylic acid, auxin and the like with higher content, has higher drought resistance, salt and alkalinity resistance and the function of promoting the growth of the seedling plant height, can be independently used as a seed soaking agent to promote the germination of seeds, and can also be used for root irrigation or spraying treatment of the seedlings by using the fermentation liquor of the endophytic fungi.

Inventors

• YIN JING
• Shen Chenjing
• LI YUJIE
• LI SHANG
• WANG SIYAO

Assignees

• 东北林业大学

Dates

Publication Date

20260512

Application Date

20250924

Claims (9)

1. 1. An endophytic fungus with functions of improving the reversibility and promoting growth of water yeast Liu Kang is characterized in that the endophytic fungus is named as Alternaria minutissima (ALTERNARIA TENUISSIMA) SLR21 and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 42057.
2. 2. Use of the endophytic fungus or the fermentation broth thereof according to claim 1 for improving drought resistance of fraxinus mandshurica.
3. 3. Use according to claim 2, wherein the endophytic fungus or a fermentation broth thereof is used for inducing the synthesis of the manchurian phenol species.
4. 4. The use according to claim 2, wherein the fermentation broth of the endophytic fungus contains polyphenols, polysaccharides, flavones, salicylic acid and auxins.
5. 5. Use of the endophytic fungus or fermentation broth thereof as claimed in claim 1 for promoting growth of fraxinus mandshurica.
6. 6. The use according to claim 5, wherein the endophytic fungus or a fermentation broth thereof is used for increasing leaf nitrogen content, soluble sugars and photosynthesis ability of fraxinus mandshurica.
7. 7. A root-irrigation or leaf-spraying agent for improving drought resistance and promoting growth of fraxinus mandshurica, comprising the endophytic fungus or fermentation broth thereof of claim 1.
8. 8. Use of the endophytic fungus or fermentation broth thereof as claimed in claim 1 for promoting germination of fraxinus mandshurica seeds.
9. 9. A seed soaking agent for promoting germination of fraxinus mandshurica seeds, comprising the endophytic fungus or a fermentation broth thereof according to claim 1.

Description

Endophytic fungus with functions of improving reversibility and promoting growth of water yeast Liu Kang and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to endophytic fungi with functions of improving the reversibility and promoting growth of water yeast Liu Kang and application thereof. Background Fraxinus mandshurica (Fraxinus mandshurica rupr.) the deciduous tree of fraxinus genus of Oleaceae family, the national class II important protection of wild plants. The wood is tough, is a precious wood tree species in China, has attractive texture, can be used for manufacturing various furniture, musical instruments, sports apparatuses, vehicles and vessels, machinery and special building materials, can be used as a medicine for bark, is a traditional medicine for treating tuberculosis and trauma, and can also be used as an insect repellent. However, the seed dormancy period is long, the propagation speed is slow, seedlings are easily affected by adverse factors such as drought, cold injury, insect damage, saline alkali and the like, and the forestation is difficult. Endophytic fungi are a microbial resource with wide application value, and mainly refer to fungi living in various tissues and organs of healthy plants in a certain stage or all stages of life history. Endophytic fungi can absorb and maintain ion balance, improve plant penetration regulation capability, maintain plant photosynthesis and water utilization efficiency, enhance plant antioxidant defense system, produce secondary metabolites or regulate signal transduction, etc. to protect host plants. The use of endophytes to overcome the damage to plants caused by abiotic stress is a very promising strategy, and researchers are increasingly focusing on the development of potential biotechnology applications for endophytes to improve drought resistance in plants. However, at present, the stress-resistant growth-promoting strain resources aiming at the fraxinus mandshurica are not fully developed, and the endophytic fungus strain with the functions of resisting drought, promoting growth, inducing phenol substance synthesis and the like is lacking in the prior art. Disclosure of Invention The present invention is directed to an endophytic fungus with functions of improving the reversibility and growth promotion of water yeast Liu Kang, so as to solve the problems set forth in the above-mentioned background art. The endophytic fungus with the functions of improving the reversibility and promoting the growth of the water yeast Liu Kang is named as Alternaria minutissima (ALTERNARIA TENUISSIMA) SLR21, and is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No. 42057, and the preservation address is North Star Xiyu No.1 No. 3 in the Korean region of Beijing, and the preservation time is 2025, 7 months and 14 days. The invention also aims to provide an application of the endophytic fungi or the fermentation liquor thereof in improving drought resistance of the fraxinus mandshurica. Preferably, the endophytic fungus or a fermentation broth thereof is used for inducing the synthesis of the fraxinol. Another object of the present invention is to provide an application of the endophytic fungus or the fermentation broth thereof in promoting the growth of fraxinus mandshurica. The fermentation broth of endophytic fungi provided by the invention contains polyphenol, polysaccharide, flavone, salicylic acid and auxin. Preferably, the endophytic fungus or the fermentation broth thereof is used for improving the antioxidant enzyme activity, leaf nitrogen content, stress-resistant substance content and photosynthesis ability of the fraxinus mandshurica. Another object of the present invention is to provide a root-irrigation agent, a leaf-spraying agent for improving drought resistance and/or promoting growth of fraxinus mandshurica, comprising the above endophytic fungus or a fermentation broth thereof. The invention also aims to provide an application of the endophytic fungi or the fermentation liquor thereof in promoting germination of the fraxinus mandshurica seeds. Another object of the present invention is to provide a seed soaking agent for promoting germination of fraxinus mandshurica seeds, comprising the above endophytic fungus or a fermentation broth thereof. The invention screens endophytic fungi with the functions of improving the reversibility and promoting growth of the aquatic yeast Liu Kang by taking the aquatic yeast willow as a material, provides a new microorganism resource for digging new strains with biological energy functions, and provides a new path for solving the problem that the aquatic yeast Liu Chenglin is difficult. The endophytic fungi has higher drought resistance and the function of promoting the growth of the seedling plant height, can be independently used as a seed soaking agent to promote seed germination, an