CN-120904904-B - Soil conditioner based on nitrification inhibition and soil acid control method

Abstract

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, wherein the soil conditioner comprises, by weight, 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid and 2.8-4.2 parts of sodium carboxymethyl cellulose. In the technical scheme of the invention, the syringic acid and the linoleic acid are used as nitrification inhibitors, so that the nitrification inhibition rate can be effectively improved, the addition of complete nitrifying bacteria can reduce the ecological niches of ammonia oxidizing archaea and ammonia oxidizing bacteria through competitive occupation, inhibit the nitrification rates of ammonia oxidizing archaea and ammonia oxidizing bacteria, and reduce the emission of N 2 O. Humic acid and hydroxyapatite can be used as pH buffer substances, regulate and control soil acidity, relieve aluminum toxicity, improve physical and chemical properties of soil, and ensure that complete nitrifying bacteria have higher activity at pH of 5.5-6.5.

Inventors

• KE QIN
• Zhao Juanyu
• SONG XIANCHONG
• TANG JIAN
• QIN ZUOYU
• SHI YUANYUAN
• HUANG XIAORUI
• JIANG HUBO
• LIU YI

Assignees

• 广西壮族自治区林业科学研究院

Dates

Publication Date

20260508

Application Date

20250731

Claims (9)

1. 1. The soil conditioner based on nitrification inhibition is characterized by comprising, by weight, 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of whole-course nitrifying bacteria, 2-5 parts of syringic acid and 2.8-4.2 parts of sodium carboxymethylcellulose; The preparation method of the syringic acid-linoleic acid modified porous hydroxyapatite comprises the following steps: A1, under the nitrogen atmosphere, the linolic acid is dissolved in absolute ethyl alcohol, porous hydroxyapatite is added for ultrasonic oscillation, water bath stirring is carried out at 70 ℃ until the solvent is evaporated, and washing, drying and grinding are carried out, thus obtaining the linolic acid modified porous hydroxyapatite; A2, dissolving syringic acid in an ethanol water solution, adding linoleic acid modified porous hydroxyapatite, ultrasonically oscillating for 2-3 hours at 40 ℃ under a nitrogen atmosphere, and drying to obtain the syringic acid-linoleic acid modified porous hydroxyapatite.
2. 2. A soil conditioner based on nitrification inhibition as claimed in claim 1, wherein in the step A1, the mass ratio of linoleic acid to porous hydroxyapatite is (3-5): 100.
3. 3. The soil conditioner based on nitrification inhibition as claimed in claim 1, wherein in the step A2, the mass ratio of the syringic acid to the linoleic acid modified porous hydroxyapatite is (1.5-2) 100, and the volume ratio of ethanol to water in the ethanol water solution is 7:3.
4. 4. A process for preparing a soil conditioner based on nitrification inhibition as claimed in any one of claims 1 to 3, comprising the steps of: Uniformly mixing syringic acid, humic acid and syringic acid-linoleic acid modified porous hydroxyapatite, adding sodium carboxymethylcellulose aqueous solution, mixing and stirring, adding whole nitrifying bacteria suspension, uniformly mixing, granulating, adding into a coating machine after granulating, spraying coating liquid for coating, and solidifying to obtain the soil conditioner based on nitrification inhibition.
5. 5. The method for preparing the soil conditioner based on nitrification inhibition as claimed in claim 4, wherein the mass concentration of the sodium carboxymethyl cellulose aqueous solution is 3-5%, and the concentration of the whole nitrifying bacteria suspension is 10 8 -10 9 CFU/mL.
6. 6. The method for preparing the soil conditioner based on nitrification inhibition as claimed in claim 4, wherein the method for preparing the coating liquid comprises the following steps: Adding sodium dodecyl sulfate into deionized water, uniformly stirring, adding dimethylaminoethyl methacrylate, butyl acrylate and ethylene glycol dimethacrylate for pre-emulsification, adding an initiator under the protection of nitrogen, and reacting for 4-6 hours at 80 ℃ to obtain coating liquid.
7. 7. The method for preparing the soil conditioner based on nitrification inhibition as claimed in claim 6, wherein the dosage ratio of the sodium dodecyl sulfate, the deionized water, the dimethylaminoethyl methacrylate, the butyl acrylate, the ethylene glycol dimethacrylate and the initiator is (1-1.3) g (200-250) mL (30-35) g (58-60) g (1.3-1.6) g (0.4-0.7) g.
8. 8. The soil acid control method is characterized by comprising the following steps of: Mixing the soil conditioner based on nitrification inhibition as claimed in claim 1 with acid soil, and then standing for restoration.
9. 9. The method for controlling acid in soil according to claim 8, wherein the addition amount of the soil conditioner based on nitrification inhibition is 0.3 to 0.5% by mass of the acid soil.

Description

Soil conditioner based on nitrification inhibition and soil acid control method Technical Field The invention belongs to the technical field of soil conditioning, and particularly relates to a soil conditioner based on nitrification inhibition and a soil acid control method. Background The long-term application of the ammonium nitrogen fertilizer leads to the generation of H + by nitrification, which is a main reason for the acidification of farmland soil. Traditional acid control methods (such as lime application) only neutralize the existing acidity and do not block the root cause of continuous acidification. Nitrification inhibitors are a generic term for compounds that delay the onset of nitrification. The application of the nitrification inhibitor can effectively slow down nitrogen conversion and nitrate accumulation, and has an emission reduction effect on N 2 O. At present, nitrification inhibitors can be divided into synthetic nitrification inhibitors and biological nitrification inhibitors, wherein the synthetic nitrification inhibitors comprise dicyandiamide (DCD), 3, 4-dimethylpyrazole phosphate (DMPP) and the like, and the synthetic nitrification inhibitors can delay the conversion of ammonium nitrogen, but have the problems of unstable performance, toxicity, easy degradation and the like, so that the synthetic nitrification inhibitors are difficult to popularize and use in a large-scale agricultural ecological system, and the environmental risk is possibly caused by volatility or residues. The biological nitrification inhibitor comprises methyl parahydroxybenzoate, 1, 9-decanediol, syringic acid and the like, is derived from plant secretion or extract, belongs to environment-friendly substances, and has good development potential. Therefore, there is a need to provide a soil conditioner based on nitrification inhibition that is green and efficient. Disclosure of Invention The invention aims to provide a soil conditioner based on nitrification inhibition and a soil acid control method, which are used for solving the problems in the background technology. The aim of the invention can be achieved by the following technical scheme: a soil conditioner based on nitrification inhibition comprises the following components in parts by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of whole-process nitrifying bacteria, 2-5 parts of syringic acid and 2.8-4.2 parts of sodium carboxymethylcellulose. In the soil conditioner based on nitrification inhibition, the syringic acid and the linoleic acid are used as the nitrification inhibitor, the nitrification inhibition rate can be effectively improved through the synergistic effect of the syringic acid and the linoleic acid, the addition of nitrifying bacteria (comammox) in the whole process can reduce the ecological niches of ammonia oxidizing archaea and ammonia oxidizing bacteria through competitive occupation, the nitrification rate of the ammonia oxidizing archaea and ammonia oxidizing bacteria is inhibited, and the emission of N 2 O is reduced. According to the invention, through the synergistic effect of the syringic acid, the linoleic acid and the whole-process nitrifying bacteria, the emission of N 2 O is effectively reduced, and the utilization rate of nitrogen in the fertilizer is improved. According to the invention, the syringic acid and the linoleic acid are loaded in the porous hydroxyapatite, on one hand, the porous hydroxyapatite is used as a carrier, so that the stability of the syringic acid and the linoleic acid can be improved, the action time is prolonged, meanwhile, the syringic acid and the linoleic acid are fat-soluble compounds, and are not easy to run off in soil, so that the action time is further prolonged, the nitrification inhibition rate is improved, and on the other hand, the coating of the fat-soluble syringic acid and the linoleic acid can improve the hydrophobicity of the porous hydroxyapatite, delay the dissolution of the hydroxyapatite in acid soil, reduce the flushing loss when meeting water, and prolong the action time. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, keep soil pH at 5.5-6.5, relieve aluminum toxicity, improve physical and chemical properties of soil, and ensure that nitrifying bacteria have higher activity in the whole process at pH of 5.5-6.5. In addition, humic acid can be used as an auxiliary carbon source through microbial decomposition and conversion, so that the abundance of nitrifying bacteria in the whole process is improved. A preparation method of a soil conditioner based on nitrification inhibition comprises the following steps: Uniformly mixing syringic acid, humic acid and syringic acid-linoleic acid modified porous hydroxyapatite, adding sodium carboxymethylcellulose aqueous solution, mixing and stirring, adding whole nitrifying bacteria suspension, uniformly mixin