CN-120918149-B - Artificial propagation method for dace with wushi's disk mouth

Abstract

The invention provides an artificial propagation method of dace with a Wushi disk mouth, and belongs to the technical field of aquatic animal propagation. The artificial propagation method of the dace with the wushi dish mouth comprises the steps of parent fish classification, parent fish cultivation, artificial spawning, natural insemination, artificial insemination, fertilized egg hatching, larval fish cultivation and the like. The oxytocic disclosed by the invention is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic yield, the fertility rate and the hatching rate are improved, and the damage to parent fish is small. The invention also defines the feeding method of the offspring seed at different stages, improves the survival rate of the offspring seed at the period of the young dace with the wushi kou, and lays technical support for the artificial breeding of the young dace with the wushi kou of the endangered species.

Inventors

• ZHANG ZHIMING
• GUAN SHIFENG
• BI JIANQI
• YUAN MINGMING
• LIU JIANFANG
• DING XIAOLIN
• ZHU HEYAN
• Bi Xiaomian
• YANG YANG
• XU LIN
• CHEN FENG
• WANG YUESONG
• DENG SEN
• Gan xing
• LI GUOLIANG
• SU QI

Assignees

• 水利部中国科学院水工程生态研究所
• 广西大藤峡水利枢纽开发有限责任公司

Dates

Publication Date

20260508

Application Date

20250821

Claims (6)

1. 1. An artificial propagation method for a dace with a wushi disc orifice is characterized by comprising the following steps: s1, collecting parent fish in the breeding season of dace on the Wu's disk mouth, and dividing the parent fish into mature parent fish, backup parent fish and postpartum female fish; S2, spawning is carried out on the mature parent fish, the backup parent fish and the postnatal female fish are respectively domesticated until the mature parent fish is developed, and spawning is carried out again; the method comprises the steps of putting a packing bag into a culture jar for 25-30 min, opening the packing bag, slowly adding water into the culture jar until the water accounts for 60-70% of the volume of the packing bag, then slowly putting the fish into the culture jar, adding 5-10 ppm povidone iodine into the jar for disinfection, and keeping the culture density of 120-180 fish/jar for 3 days without feeding, starting daily culture on 4 th day and starting intensified culture every 3 months; the daily cultivation is that grass carp feed crushed materials with the weight of 2% -3% of the weight of 1 fish are fed for 18:00 times every day, the diameter of a cultivation cylinder is 2m, and the depth of the cultivation cylinder is 1m; The reinforced cultivation is that feeding is carried out for 1 time every day in the range of 8:00 and 18:00, and the feeding amount of each time is 2-3% of the total amount of pond cultivation; The induced spawning is that LHRH-A 2 2.8.8-3.2 mug/kg is injected into mature female parent fish, and mature male parent fish is not injected; Injecting LHRH-A 2 14.5.5-15.5 mug/kg+DOM 4.8-5.2 mg/kg+HCG 1900-2100IU/kg into the mature female parent fish after 10 hours, and simultaneously injecting LHRH-A 2 -8 mug/kg+DOM 2.3-2.7 mg/kg+HCG 900-1100 IU/kg into the mature male parent fish; S3, simulating a habitat for natural insemination of parent fish to be produced, wherein the operation of simulating the habitat comprises the steps of mixing female parent fish and male parent fish according to a ratio of 3:2, controlling the water temperature to be 18-19 ℃, and controlling dissolved oxygen to be 6-9 mg/L, and completing insemination after 6-8 hours to obtain fertilized eggs and postpartum parent fish; after the second needle injection is completed for 16 hours, checking whether female parent fish completes spawning, and picking out non-spawning parent fish; S4, carrying out artificial insemination on the parent fish which does not produce the sperm to obtain fertilized eggs and postpartum parent fish, wherein the artificial insemination comprises the steps of extruding female fish, extruding the eggs to a dry and clean container, extruding male fish, extruding semen, flushing the semen onto the eggs by using saline with the mass fraction of 0.85-0.95%, stirring to obtain a sperm-egg mixture, adding 2-3 times of water to activate sperms, stirring for 2-3 min, flushing with clear water for 3-5 times, and washing mucus and impurities to obtain fertilized eggs; S5, placing fertilized eggs in a hatching frame for hatching to obtain larval fish; s6, culturing the young fish to obtain the young fish; S7, culturing juvenile fish to obtain juvenile fish; The hatching conditions are that the laying density of fertilized eggs is 0.8 ten thousand-1 ten thousand grains/m 2 , the water body exchange times are 10-12 times/day, the water temperature is 18-20 ℃, and hatching out films is 84-90 hours, so that the larval fish is obtained.
2. 2. The artificial propagation method according to claim 1, wherein the parent fish is classified into a female fish with obvious striae gravidarum on the rear side of pectoral fin, a male fish with a weight of 3g or more and an egg with a weight of 5g or more, which can be extruded into semen, and a mature parent fish, and the other parent fish with a back.
3. 3. The artificial propagation method according to claim 1, wherein the water temperature, dissolved oxygen, pH, ammonia nitrogen and nitrite index are monitored daily in the domestication stage of the backup parent fish and the postnatal female fish, water is changed 1 time every 3 days, and the water amount is 25-35% for each time, so that the water quality is kept good.
4. 4. The artificial propagation method according to claim 1, wherein the method of domesticating the postnatal parent fish subjected to natural insemination or artificial insemination is the same as the daily cultivation conditions in S2.
5. 5. The artificial propagation method of claim 1, wherein the breeding of the fries is that dead eggs and dead seedlings are removed in the morning and evening for 0-5 d without feeding after membrane rupture, 1 time of artemia is fed for 6-15 d in an excessive amount every 6h, the fries are transferred to a plastic water tank with the length of 2m and the width of 1m and the depth of 0.6m in 16d, water inlet and water outlet are independent, the water depth is controlled to be 0.4m, each water tank is bred and bred for 1 ten thousand feet, 1 time of artemia is fed for 16-25 d in an excessive amount every 8h, residual baits and faeces are sucked out 30min after each feeding is completed, the water exchange time is controlled to be 5-7 times per day, and the fries are obtained in 26 d.
6. 6. The artificial propagation method according to claim 1, wherein the young fish is cultivated by transferring the young fish into a cultivation jar with the diameter of 2m and the depth of 1m for 27d, controlling the water level to be 0.6-0.7 m, controlling the cultivation density to be 1500-2000 tails/jar, feeding 1-time mixture of the fairy shrimp and the grass carp crushed material to 8:00 and 19:00 a day for 27-30 d, feeding 1-time mixture of the fairy shrimp and the grass carp crushed material to 8:00 and 19:00 a day for 31-33 d in a mixing ratio of 1:1, feeding 1-time grass carp crushed material to 8:00 and 19:00 a day for 34d, and obtaining the young fish after 60d, and performing domestication by referring to the daily cultivation mode of S2.

Description

Artificial propagation method for dace with wushi's disk mouth Technical Field The invention relates to the technical field of aquatic animal breeding, in particular to an artificial breeding method of a dace with a Wushi dish mouth. Background The dace (Discocheilus wui) belongs to the order of cyprinid (Cypriniformes), the family of Cyprinid (CYPRINIDAE) and the genus dace (Discocheilus) and is a rare or endangered special fish, the distribution range is narrow, the dace is only distributed in Guangxi Tiane county, fengshan county, legend county and the like, the dace is a stream gravel substrate environment with turbulent water flow, clear water quality and high dissolved oxygen, and the dace is benthic scratch fish and is eaten by epiphytic algae, organic chips and the like. As the habitat of the Jun's disk mouth is karst landform, the habitat is easily damaged, and the field population resources are obviously reduced. Under natural conditions, the wushi population dace needs specific environmental conditions to successfully reproduce, such as specific water flow speed, water temperature range (usually in spring and summer), clear water quality, proper gravel spawning sites and the like, and the wushi population dace has extremely low reproduction rate under natural conditions, so that the population and resources of the wushi population dace need to be protected by artificial reproduction, proliferation and release. However, artificial propagation of the dace on the Wu's disk has the technical bottlenecks that gonad development of female and male parent fishes is asynchronous or unsatisfactory, high-quality parent fishes with consistent maturity are difficult to obtain, and because of special propagation physiology, accurate grasp of the gonad maturity and optimal oxytocic time is very difficult, and the oxytocic hormone (such as HCG, LRH-A 2, DOM and the like) of other fishes is used for referencing that the effect of the oxytocic hormone on the dace on the Wu's disk is poor, so that the specific hormone types, doses and combinations effective on the female parent fishes need to be explored. No mature, stable and repeatable full artificial propagation technology for the dace of the wushi's disk mouth exists in the prior art. Disclosure of Invention In view of the above, the present invention provides an artificial propagation method of dace with the five's mouth to solve the above-mentioned problems. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides an artificial propagation method of a dace with a wushi disk mouth, which is characterized by comprising the following steps: s1, collecting parent fish in the breeding season of dace on the Wu's disk mouth, and dividing the parent fish into mature parent fish, backup parent fish and postpartum female fish; S2, spawning is carried out on the mature parent fish, the backup parent fish and the postnatal female fish are respectively domesticated until the mature parent fish is developed, and spawning is carried out again; the method comprises the steps of putting a packing bag into a culture jar for 25-30 min, opening the packing bag, slowly adding water into the culture jar until the water accounts for 60-70% of the volume of the packing bag, then slowly putting the fish into the culture jar, adding 5-10 ppm povidone iodine into the jar for disinfection, and keeping the culture density of 120-180 fish/jar for 3 days without feeding, starting daily culture on 4 th day and starting intensified culture every 3 months; The daily cultivation is that grass carp feed crushed materials with the weight of about 2% -3% of the weight of the fish are fed for 1 time at a ratio of 18:00 per day, wherein the diameter of a cultivation cylinder is 2m, and the depth of the cultivation cylinder is 1m; The reinforced cultivation is that feeding is carried out for 1 time every day in the range of 8:00 and 18:00, and the feeding amount of each time is 2-3% of the total amount of pond cultivation; The induced spawning is as follows: Injecting LHRH-A 2 2.8.8-3.2 mug/kg into mature female parent fish, and not injecting into mature male parent fish; Injecting LHRH-A 2 14.5.5-15.5 mug/kg+DOM 4.8-5.2 mug/kg+HCG 1900-2100IU/kg into the mature female parent fish after 10 hours, and simultaneously injecting LHRH-A 2 -8 mug/kg+DOM 2.3-2.7 mg/kg+HCG 900-1100 IU/kg into the mature male parent fish; S3, simulating a habitat for natural insemination of parent fish to be produced, wherein the operation of simulating the habitat comprises the steps of mixing female parent fish and male parent fish according to a ratio of 3:2, controlling the water temperature to be 18-19 ℃, and finishing insemination after 6-8 hours to obtain fertilized eggs and postpartum parent fish; s4, carrying out artificial insemination on the parent fish which does not produce the sperm to obtain fertilized eggs and postpartum parent fish, wherei