CN-120918705-B - Patch type ultrasonic biomarker integrated sensor and detection method

Abstract

The invention is suitable for the technical field of high-end flexible sensing preparation and multi-mode sensing detection, and provides a patch type ultrasonic biomarker integrated sensor and a detection method, wherein the sensor comprises a patch type ultrasonic transducer array, a sensor module and a sensor module, wherein the patch type ultrasonic transducer array is used for monitoring myobone diseases in real time, and realizing myobone structure-oriented functional multi-parameter multi-mode data information fusion detection and analysis; the invention can systematically evaluate the coordination condition of the structure, mechanics, metabolic inflammation level and nerve function activities of musculoskeletal, and provides a new means for early identification, quantitative evaluation and personalized intervention of musculoskeletal diseases, thereby having important clinical and industrial values.

Inventors

• PENG JUE
• KONG FANKAI

Assignees

• 深圳大学

Dates

Publication Date

20260512

Application Date

20250807

Claims (6)

1. 1. A patch-type ultrasonic biomarker integrated sensor, comprising: the patch type ultrasonic transducer array is used for realizing ultrasonic functional imaging of muscle tissues and realizing structure-functional multi-parameter multi-mode data information fusion detection and analysis facing to muscle bones; the microneedle electrode array is used for in-situ detection of the biomarker and acquisition of physiological electric signals; the flexible electronic circuit is used for bearing and connecting the patch type ultrasonic transducer array and the microneedle electrode array; An acoustic coupling adhesive layer for skin attachment and acoustic coupling; the microneedle electrode array consists of orderly arranged conductive sparse microneedles, the length of each microneedle is 500-800 mu m, and the shape of each microneedle is a conical shape, a cylindrical cone composite structure or a hollow structure; The surface of the microneedle electrode array is fixed with specific recognition molecules through functional modification; The specific recognition molecule is selected from an antibody, an enzyme membrane or an electrode response membrane; The biomarker for detection of the microneedle electrode array comprises inflammatory factors and metabolic indexes; the inflammatory factor is selected from the group consisting of IL-6, TNF-alpha and CRP; the metabolic index is selected from lactic acid, creatine kinase and pH value; the physiological electrical signal comprises an electromyographic signal; The flexible electronic circuit is composed of a flexible substrate material and stretchable interconnection conductors; The flexible substrate material is selected from SEBS, PC, PI, TPU, PDMS or Eco-flex; the stretchable interconnection conductor adopts a metal wire with a serpentine, horseshoe-shaped or wave-shaped structure.
2. 2. The integrated patch-type ultrasonic biomarker sensor according to claim 1, wherein the patch-type ultrasonic transducer array is composed of a one-dimensional or two-dimensional piezoelectric array, and the piezoelectric material of the patch-type ultrasonic transducer array includes PZT, PMN-PT, 1-3 composite material or piezoelectric polymer.
3. 3. The integrated patch-type ultrasound biomarker sensor according to claim 1, wherein the ultrasound functional imaging includes two-dimensional B-mode imaging, multi-angle plane wave excitation and high-speed echo reception, doppler blood flow imaging, and shear wave velocity estimation and elastic modulus imaging.
4. 4. The integrated patch-type ultrasonic biomarker sensor according to claim 1, wherein the acoustic coupling adhesive layer is a hydrogel adhesive layer having a thickness of 200-300 μm.
5. 5. A biomarker detection method employing the patch-type ultrasonic biomarker-integrated sensor according to any of claims 1 to 4, characterized in that the method comprises the steps of: attaching a patch type ultrasonic biomarker integrated sensor to a target muscle area to be monitored; The hydrogel adhesion layer realizes the fitting and acoustic coupling with the skin, and the microneedle electrode array penetrates through the stratum corneum and contacts interstitial fluid; Positioning a muscle injury area according to flexible ultrasonic functional imaging, and monitoring biomarkers of interstitial fluid seeds by using a microneedle electrode; collecting electromyographic signals by using a microneedle electrode array, and analyzing the nerve function state of muscle tissues to obtain a muscle fatigue state; and comprehensively evaluating the health state of the muscle tissue based on ultrasonic functional imaging, microneedle biomarkers and nerve functional monitoring to generate a comprehensive analysis report.
6. 6. The method of claim 5, wherein the analysis-by-synthesis report includes a structural image, an elastogram, an inflammation level, and an myoelectric curve.

Description

Patch type ultrasonic biomarker integrated sensor and detection method Technical Field The invention relates to the technical field of flexible sensing preparation and multi-mode sensing detection, in particular to a patch type ultrasonic biomarker integrated sensor and a detection method. Background Sarcopenia and musculoskeletal system diseases (e.g., muscle strain, tendon injury, and musculoskeletal diseases associated with muscle fatigue) severely affect quality of life. It was found that in myopenia patients, particularly in elderly hip fracture patients, the levels of markers such as C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) were elevated. Furthermore, related studies indicate that in sarcopenia patients, the levels of pro-inflammatory factors, such as TNF- α and IL-6, both appear to be elevated. Meanwhile, in modern industrial labor, a working mode of low load, fast rhythm, high repetition, long time and forced body position exists, and the forced body position is extremely easy to induce local muscle fatigue, and can cause musculoskeletal injury after long-time accumulation. Currently, clinical diagnosis of musculoskeletal injury and sarcopenia is primarily dependent on imaging means, such as Magnetic Resonance Imaging (MRI), X-rays, and the like. However, such tools are less sensitive at the early stages of the disease and often lack a direct correspondence with clinical symptoms. In particular, conventional imaging tools fail to achieve effective detection at early stages of musculoskeletal injury where no significant structural changes have occurred. In this context, biomarker-based detection methods are becoming an important complementary tool. Inflammatory factors, oxidative stress products, metabolic indexes and the like can reflect early changes of the microenvironment of the muscle system, and are potential means for realizing early warning and continuous monitoring of muscle diseases. The national institute of occupational safety and health also indicates that by detecting biomarkers in tissue fluids, it is possible to intervene before irreversible damage to the tissue occurs. The existing biomarker detection means depend on blood sampling or laboratory analysis, so that wearable, local and real-time detection is difficult to realize. Meanwhile, an integrated sensing system capable of simultaneously monitoring muscle anatomy, mechanical function, metabolic inflammation level and neural activity synergy is not yet available. Traditional assessment means such as MRI, electromyography or serological detection can provide certain information, but have the limitations that equipment is expensive, real-time monitoring cannot be carried out or only the whole body state is reflected, and the clinical requirements of local, continuous and multi-parameter monitoring of muscle tissues are difficult to meet. Therefore, a multi-mode sensing platform with minimally invasive puncture capability, electrochemical detection function and flexible mechanical imaging capability is urgently needed, and the multi-mode sensing platform can be used for cooperatively monitoring the structure, the function and the inflammation marker of muscle tissue in an actual application scene. In recent years, the rapid development of wearable flexible sensors and micro-needle technology provides a new idea for minimally invasive detection of shallow tissues. The microneedle electrode can penetrate the stratum corneum of skin without causing pain, effectively collect interstitial fluid (ISF) or directly detect local biochemical markers and physiological electric signals in situ, such as inflammatory factors like IL-6 and TNF-alpha, and metabolites like lactic acid and pH value, and myoelectricity. Meanwhile, the ultrasonic patch has good skin fit and high resolution imaging capability, is used for continuously monitoring the muscle structure and mechanical change, and is particularly suitable for muscle function imaging, such as B-mode imaging, elastography, doppler imaging and the like, so as to realize multi-parameter evaluation of tissue hardness, blood supply, structure and the like. However, the prior art has the following disadvantages in large part: 1. The existing muscle injury detection technology is mostly in a single mode, or can only analyze inflammation indexes through body fluid sampling, or can only acquire tissue images (such as ultrasound and MRI), or can only acquire tissue physiological electric signals (such as myoelectricity), and synchronous evaluation of muscle structures and biochemical states cannot be realized; 2. the invasiveness is strong, the wearability is poor, the biomarker detection usually depends on invasive means such as blood drawing, biopsy and the like, is not suitable for continuous dynamic monitoring, and can not collect target area data in real time in daily activities; 3. the early diagnosis capability is insufficient, for example, the imaging technologies such a