CN-120924686-B - Molecular marker related to Ningxiang pig feed meat ratio character and application thereof

Abstract

The invention relates to the technical field of biology, in particular to a molecular marker related to the feed-meat ratio character of Ningxiang pigs and application thereof. The molecular markers comprise SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 97217694 th site from the 5 'end on chromosome 1 of Sus Scrofa Build11.1 of the reference genome, and is C or T, and the SNP2 molecular marker corresponds to a 97590304 th site from the 5' end on chromosome 1 of Sus Scrofa Build11.1 of the reference genome, and is G or T. The invention obtains the molecular marker obviously related to the feed-meat ratio character of the Ningxiang pigs, provides guidance for breeding of the Ningxiang pigs by using the molecular marker, can accurately and efficiently predict feed-meat ratio and parting, identify and screen pigs with low feed-meat ratio, and improve breeding efficiency.

Inventors

• LI FENGNA
• GAO JINGXIA
• DENG XIAOXIAO
• GUO QIUPING
• XU KANG
• SUN JINGCHUN
• YIN YULONG

Assignees

• 中国科学院亚热带农业生态研究所

Dates

Publication Date

20260512

Application Date

20250910

Claims (2)

1. 1. The application of the molecular marker related to the Ningxiang pig feed specific trait in detection or auxiliary detection of the Ningxiang pig feed specific trait is characterized in that the molecular marker is SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 97217694 th site from the 5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or T, and the SNP2 molecular marker corresponds to a 97590304 th site from the 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or T.
2. 2. The application of the molecular marker related to Ningxiang pig feed ratio traits in early detection of Ningxiang pig feed ratio is characterized in that the molecular markers are SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 97217694 th site from a5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or T, and the SNP2 molecular marker corresponds to a 97590304 th site from a 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or T.

Description

Molecular marker related to Ningxiang pig feed meat ratio character and application thereof Technical Field The invention relates to the technical field of biology, in particular to a molecular marker related to the feed-meat ratio character of Ningxiang pigs and application thereof. Background The global pork industry is currently undergoing large-scale and intensive transformation, and the feed cost becomes a core factor limiting the industrial benefit. The feed to meat ratio (Feed to Gain Ratio, F: G) is a key indicator for the animal to convert feed into weight gain, and is a core parameter for expressing feed conversion efficiency (Feed Conversion Efficiency, FCE) and directly determines the feed consumption per unit output. The reduction of F and G can obviously reduce the production cost, and is a core break for improving the cultivation economy and the sustainability. The traditional F-G improvement method mainly depends on phenotype selection, and has two main bottlenecks, namely that the F-G belongs to complex characters, the phenotype data acquisition period is long and is easy to be interfered by environment, so that the accuracy is insufficient, and the traditional method cannot accurately position a key genome region, so that the genetic improvement efficiency is limited. As a third generation genetic marker, single Nucleotide Polymorphism (SNP) has the characteristics of high density, high stability and wide genome distribution. The genetic basis of F: G can be systematically resolved by whole genome association analysis (GWAS). The technology can directly correlate genotype and phenotype variation, realizes accurate analysis of complex characters, and promotes large-scale application of molecular assisted breeding. The Chinese patent publication No. CN 120210383A discloses a method for assisting in identifying the feed-meat ratio of pigs and a molecular marker thereof, but aims at large white pigs, SNP molecular markers related to the feed-meat ratio characteristics of Ningxiang pigs are not disclosed in the prior art, and the large white pigs and the Ningxiang pigs are two different types of pigs with obvious difference, and the large white pigs have high growth speed, high feed conversion rate, slower growth speed of the Ningxiang pigs and low feed conversion rate. Although the traits are the same, the traits are affected by a plurality of sites, so that large white pigs (lean type) and Ningxiang pigs (fat type) have obvious differences in SNP molecular markers related to the feed-to-meat ratio traits. Disclosure of Invention Aiming at the defects in the prior art, the invention aims to provide a molecular marker related to the feed-meat ratio character of Ningxiang pigs and application thereof. The marker can assist in screening the F-G characters of Ningxiang pigs and improve the breeding of the excellent characters of Ningxiang pigs. In order to achieve the aim of the invention, the invention adopts the following technical scheme: In a first aspect, a molecular marker related to the Ningxiang pig feed meat ratio character is provided, wherein the molecular marker comprises SNP1 and SNP2, the sequence of nucleic acid containing the SNP1 molecular marker is shown as SEQ ID No.1, the SNP1 molecular marker corresponds to a 97217694 th site from the 5 'end on chromosome 1 with a reference genome of Sus Scrofa building 11.1, and is C or T, the sequence of nucleic acid containing the SNP2 molecular marker is shown as SEQ ID No.2, and the SNP2 molecular marker corresponds to a 97590304 th site from the 5' end on chromosome 1 with a reference genome of Sus Scrofa building 11.1, and is G or T. In a second aspect, the application of the SNP molecular marker in detection or auxiliary detection of the Ningxiang pig feed conversion ratio character is provided. In a third aspect, the application of the SNP molecular marker in Ningxiang pig feed to meat ratio early screening is provided. In a fourth aspect, the application of the SNP molecular marker in Ningxiang pig molecular marker assisted breeding is provided. In a fifth aspect, the application of the SNP molecular marker in Ningxiang pig breed screening is provided. Further, there is a linkage effect at both sites of SNP1 and SNP2, and the genotype combination of CC/GG is a favorable genotype. The beneficial effects of the invention are as follows: (1) The invention obtains the molecular marker obviously related to the feed-meat ratio of the Ningxiang pigs, provides guidance for breeding of the Ningxiang pigs by using the molecular marker, can accurately and efficiently predict the feed-meat ratio and parting, identify and screen pigs with low feed-meat ratio, and improve the breeding efficiency. (2) The invention provides a sequencing primer pair and a KASP primer pair for detecting SNP molecular markers related to Ningxiang pig feed conversion ratio, the primer pair has strong specificity and high accuracy, can accurately obtain sequences contai