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CN-120938830-B - Photo-thermal stability nano lipid carrier system and preparation method and application thereof

CN120938830BCN 120938830 BCN120938830 BCN 120938830BCN-120938830-B

Abstract

The invention provides a photo-thermal stability nano lipid carrier system, a preparation method and application thereof, and relates to the technical field of nano preparations. The lipid carrier comprises an active substance, hydrogenated lecithin, jojoba seed oil, SPECTRASTATTM HCP, an emulsifying agent and a chelating agent, wherein the active substance is resveratrol. The lipid carrier has good stability, is stable under the conditions of dilution, centrifugation and the like, and can be stored for a long time under severe environments. The lipid carrier is used for coating indissolvable substances such as resveratrol, so that the water solubility of the substances can be remarkably improved, and the problem of difficult dissolution is solved. In addition, the encapsulation of the lipid carrier can effectively isolate the influence of environmental factors such as oxygen, illumination and the like, can improve the stability of active substances, and can effectively improve the color-changing problem. The nanoscale lipid carrier is easier to penetrate through skin barriers and penetrate deep into skin, and is more beneficial to the efficacy of transferring active substances when applied to a preparation.

Inventors

  • ZHANG ZEQI
  • YANG PINGSHUN
  • ZHAO JUN
  • LI CHUXIN
  • HAN CHUNXIA
  • HUANG GANGFU
  • LIANG JUNYI

Assignees

  • 东莞市容大生物科技有限公司

Dates

Publication Date
20260508
Application Date
20250916

Claims (9)

  1. 1. A photo-thermal stability nano lipid carrier system is characterized by comprising the following raw materials, by weight, 0.1% -8% of resveratrol, 0.5% -5% of hydrogenated lecithin, 5% -15% of jojoba seed oil, 0.1% -2% SPECTRASTATTM HCP, 1% -20% of an emulsifying agent and 0.005% -0.5% of a chelating agent, wherein the total amount of water is up to 100%, and the hydrogenated lecithin is PHOSPHOLIPON H; the preparation method of the photo-thermal stability nano lipid carrier system comprises the following steps: s1, mixing an emulsifying agent, a chelating agent and water to obtain a water phase, wherein the mixing temperature is 60-80 ℃; s2, mixing hydrogenated lecithin, jojoba seed oil and resveratrol to obtain an oil phase, wherein the mixing temperature is 60-80 ℃; S3, mixing the water phase and the oil phase, homogenizing, and adding SPECTRASTATTM HCP, wherein the mixing parameters are that the shearing rate is 8000-9500rpm, the time is 1-3min, the homogenizing parameters are that the pressure is 200-1000bar, the temperature is 10-80 ℃, and the times are 5-10 times.
  2. 2. The photo-thermal stable nano-lipid carrier system according to claim 1, wherein the emulsifier is selected from at least one of polysorbate-80, decaglycerol monolaurate, sodium stearyl glutamate, PEG-8 stearate or PEG-2 cetyl alcohol ether.
  3. 3. The photo-thermal stable nano-lipid carrier system according to claim 2, wherein the emulsifier is polysorbate-80.
  4. 4. The photo-thermal stable nano-lipid carrier system according to claim 1, wherein the chelating agent is selected from at least one of disodium edetate, butylated hydroxyanisole, vitamin E, sodium metabisulfite or 2, 6-di-tert-butyl-p-cresol.
  5. 5. The photo-thermal stable nano-lipid carrier system according to claim 4, wherein the chelating agent is disodium edetate.
  6. 6. The photo-thermal stability nano-lipid carrier system according to claim 1, wherein the nano-lipid carrier system comprises, by mass, 0.1% -5% of resveratrol, 0.5% -3% of hydrogenated lecithin, 5% -12% of jojoba seed oil, 0.1% -1.5% SPECTRASTATTM HCP%, 1% -10% of an emulsifier and 0.01% -0.1% of a chelating agent, and the total amount of water is up to 100%.
  7. 7. A method for preparing a photo-thermo stable nano lipid carrier system according to any of claims 1-6, characterized by comprising the steps of: s1, mixing an emulsifying agent, a chelating agent and water to obtain a water phase, wherein the mixing temperature is 60-80 ℃; s2, mixing hydrogenated lecithin, jojoba seed oil and resveratrol to obtain an oil phase, wherein the mixing temperature is 60-80 ℃; S3, mixing the water phase and the oil phase, homogenizing, and adding SPECTRASTATTM HCP, wherein the mixing parameters are that the shearing rate is 8000-9500rpm, the time is 1-3min, the homogenizing parameters are that the pressure is 200-1000bar, the temperature is 10-80 ℃, and the times are 5-10 times.
  8. 8. The method according to claim 7, wherein, In the step S1 and the step S2, the temperature of the mixing is 60-80 ℃; In the step S3, the mixing parameters are that the shearing rate is 8000-9500rpm and the time is 1-3min, the homogenizing parameters are that the pressure is 200-1000bar, the temperature is 10-80 ℃ and the times are 5-10 times.
  9. 9. Use of the photo-thermo stable nano lipid carrier system according to any of claims 1-6 for the preparation of cosmetics.

Description

Photo-thermal stability nano lipid carrier system and preparation method and application thereof Technical Field The invention relates to the technical field of nano preparations, in particular to a photo-thermal stability nano lipid carrier system, a preparation method and application. Background The lipid carrier is a substance with active ingredients embedded in a lipid material, and prevents the active ingredients from being damaged by the environment (temperature, oxygen, pH, enzymes, etc.), thereby improving the bioactivity and utilization rate of the active ingredients. The lipid carrier has a structure similar to a cell membrane, is a concentric vesicle composed of phospholipid bilayer, and can be loaded with water-soluble, fat-soluble and oil-water insoluble active ingredients. The water-soluble component is encapsulated in the water cavity, while the lipophilic component is encapsulated between the lipid bilayer. In addition, the lipid carrier has the advantages of high bioavailability, convenient absorption, high stability and the like, and is widely applied to the aspects of medicines, cosmetics, foods, genetic engineering and the like. The lipid carrier has unique properties of ① amphipathy, the lipid carrier can encapsulate lipid-soluble substances and water-soluble substances, ② is slow-release, active substances encapsulated by the lipid carrier can be slowly and continuously released, the availability of the active substances is improved, ③ is protective, a plurality of bioactive substances can be inactivated under the influence of temperature, light and the like, the lipid carrier can protect the active substances from being denatured and inactivated like clothes, ④ has a moisturizing effect in skin care products, the lipid carrier has a good moisturizing effect on skin, ⑤ has a skin care effect, and unsaturated phospholipids in the lipid carrier can repair damaged skin cells and can obviously improve rough skin. Particularly, with the development of functional skin care products in recent years, the demands for effectiveness and usefulness are increased, and the lipid carrier has wide application prospect in the field of skin care products due to good compatibility of the lipid carrier and skin cell structures. The research proves that various insoluble plant active ingredients have remarkable health value, such as pterostilbene can play roles in whitening, resisting cancer and the like through a powerful antioxidant, anti-inflammatory and antitumor mechanism, and dioscin has estrogen-like regulation function, and resveratrol shows anti-aging, cardiovascular protection and neuroprotective effects. However, the components have the defects of poor water solubility, easy oxidative degradation and the like, so that the oral bioavailability is low, and the traditional controlled release technology is limited by high cost and insufficient adaptability to gastrointestinal tract pH fluctuation, so that the clinical transformation application of the controlled release technology is severely limited. Therefore, the development of a novel nano delivery system with high stability and high bioavailability has a key meaning for breaking through the application bottleneck of indissolvable active substances. Resveratrol, also called as stilbene triphenol, is a plant antitoxin produced by plants for resisting external stimuli such as ultraviolet rays, fungi and virus infection or mechanical injury, has the function of scavenging free radicals, has the function of resisting oxidation and regulating, can achieve the effect of reducing inflammatory reaction by regulating cytokines, has better whitening effect, and has various physiological functions of resisting cancer, protecting cardiovascular, regulating estrogen, protecting nerves and liver, resisting radiation, relieving cough and asthma, reducing blood pressure, improving microcirculation, treating AIDS, shock and the like. However, resveratrol is unstable in nature, is easy to decompose when exposed to heat, is poor in water solubility, so that the resveratrol is difficult to apply in cosmetics, for example, oil and water are difficult to dissolve, the formula is difficult to apply, and the resveratrol is easy to deactivate under illumination and oxygen, and color change problems and the like occur. Based on this, researchers in the field try to develop a lipid carrier, and encapsulate active ingredients with indissolvable resveratrol and poor stability, so that the active ingredients exert better efficacy. However, there are many problems in the existing researches, for example, the problem that the resveratrol content in the ① lipid carrier is low, which results in difficulty in sufficient addition (the resveratrol content is 0.0397%, "high-pressure micro-jet method is used for preparing resveratrol nano-lipid carrier", chen Qiongling and Liu Gongzhi; the core agrology report 2015,29 (5): 0916-0924), and more researches do not clearly indicate the w