CN-120938878-B - Fermentation active matter with relieving and repairing functions and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly provides a fermentation active substance with a relieving and repairing effect, and a preparation method and application thereof. The fermentation active substance is prepared by performing two-stage fermentation on a sea cabbage extract, a kava extract and a sea fennel extract by a composite strain (lactobacillus plantarum CGMCC1.557: pichia pastoris ATCC 76273=3:1), wherein the two-stage fermentation is performed for 72 hours at the temperature of 28 ℃ to pH=5.5, then performed for 48 hours at the temperature of 32 ℃ to pH=6.8, and the fermentation active substance is obtained by ultrafiltration sterilization. The active substance is compounded with rhamnose, pentanediol and hexanediol to form a plant composite essence, the slow release of the active substance is realized through a rhamnose-pentanediol hydrogen bond network, and a pentanediol-hexanediol system provides hyposensitization and corrosion prevention. Experiments show that the essence can reduce the erythema area by 47.1-53.6%, improve the water content of the horny layer by 59.4-65.9%, shorten the reddening time to 2-5 minutes, and has no sensitization, and is obviously superior to a single plant extract compounding scheme.

Inventors

• DENG YUHANG
• ZHOU ZHIRONG
• ZHENG JIAZHEN

Assignees

• 广州妍姿风韵化妆品有限公司

Dates

Publication Date

20260505

Application Date

20250903

Claims (5)

1. 1. The plant composite essence is characterized by comprising, by mass, 6.0-10.0% of fermentation active substances, 2.0-4.0% of rhamnose, 3.5-5.0% of pentanediol, 0.3-0.5% of hexanediol and the balance of water; The preparation method of the fermentation active substance comprises the following steps: s1, mixing the sea cabbage extract, the kava extract and the sea fennel extract according to the mass ratio of 4-7:4-6:4-7, and inoculating a composite strain; S2, anaerobic fermentation at 28 ± 1 ℃ for 72 hours to ph=5.5; s3, performing micro-aerobic fermentation at 32 ℃ plus or minus 1 ℃ for 48 hours until the pH value is=6.8; S4, ultrafiltering through a 10kDa membrane and sterilizing; the composite strain consists of lactobacillus plantarum CGMCC1.557 and pichia pastoris ATCC76273 according to the proportion of 3:1, and the inoculation amount is 1.5+/-0.2% of the mass of the mixture.
2. 2. The plant composite essence according to claim 1, wherein the plant composite essence consists of 7.5% of fermentation active substances, 2.5% of rhamnose, 5.0% of pentanediol, 0.5% of hexanediol and the balance of water according to mass ratio.
3. 3. A method for preparing the plant composite essence according to claim 1, which is characterized by comprising the following steps: S1, dissolving rhamnose in warm water at 60 ℃, wherein the rhamnose accounts for 30% of the total water; s2, adding pentanediol and hexanediol, and homogenizing at 2000rpm for 5 minutes; S3, adding the fermentation active substance as claimed in claim 1, and supplementing water to 100%.
4. 4. The use of the plant composite essence according to claim 1 for preparing a soothing and repairing cosmetic.
5. 5. The use of the plant composite essence according to claim 1 for repairing ultraviolet damage.

Description

Fermentation active matter with relieving and repairing functions and preparation method thereof Technical Field The invention relates to the technical field of cosmetics, in particular to a fermentation active substance with relieving and repairing functions and a preparation method thereof. Background In the technical field of cosmetics, the essence is used as a core skin care product and is mainly used for relieving skin redness and swelling, resisting inflammation and repairing, and helping skin recover healthy and compact normal state. The efficacy is mainly dependent on plant extracts as active ingredients, which are acted upon by water-based carriers. However, there are significant drawbacks in the prior art, leading to an undesirable healing effect of the serum, with concomitant safety risks. Specifically, the existing essence generally adopts a combination of high-concentration and various plant extracts to pursue a multifunctional repairing effect. For example, in patent CN109549893B, various components including purslane extract, chlorella extract, broccoli extract, jiang Huagen extract, and elm extract are added to the essence. The strategy of adding a large amount not only greatly improves the production cost, but also causes poor skin absorptivity due to lower biocompatibility of the plant extract. While the active component is increased, the healing effect does not show a positive correlation enhancement, on the contrary, the high concentration of active substances requires to be adapted with more powerful antibacterial preservative components, which may pose a threat to skin safety, such as triggering irritation or allergic response. In addition, common as main efficacy components are kale extract, kava extract and fennel extract, which are limited in efficacy and require high concentration addition in the prior art. Taking patent CN108553596a as an example, the soothing composition is added with 10-18% of fennel extract, and the high dose is easy to stimulate skin, but the repairing and soothing effects are not significant. Similarly, while there are advantages to kale extract (containing glucosinolates, having anti-aging and anti-inflammatory properties), kava extract (containing kavalactones, modulating neurogenic inflammation) and fennel extract (promoting keratinocyte regeneration and antioxidant), when used alone or in simple formulations, synergistic effects cannot be achieved due to ingredient compatibility problems, but may result in increased skin burden. To verify these drawbacks, experimental methods are often used in the prior art to evaluate the performance of the concentrate. For example, skin redness and swelling is induced by histamine or other irritants (e.g., histamine phosphate solution), one method is to apply a blank placebo, essence or hormone cream, respectively, before the irritants are applied, and observe the time to redness and swelling, and another method is to apply the irritants to induce redness and swelling, and then apply test samples to evaluate the soothing effect. These experiments highlight the deficiencies of the existing essence in rapid redness and restoration efficiency, and as the test results show, the redness-removing time of the traditional essence is as long as 8-10 minutes, which is far lower than the ideal effect. Meanwhile, rhamnose is taken as a potential synergistic component, the slow release effect of rhamnose is not fully utilized in the prior art, and the performance optimization of the essence is further limited. In summary, the existing essence technology faces three major core problems: (1) The economy is poor, and the cost is high due to the dependence on a plurality of high-concentration plant extracts; (2) The efficacy is low, the biocompatibility is low, the absorptivity is insufficient, and the repairing effect is not proportional to the concentration of the components; (3) Safety risk that the high-activity component needs to be strongly preserved, and the skin irritation and sensitization possibility are increased. These problems are in need of an innovative solution to achieve efficient, safe and economical soothing and repairing effects by optimizing the composition and preparation process of the ingredients. Disclosure of Invention In order to solve the problems and achieve the aim of the invention, the invention firstly provides a fermentation active substance which is prepared by compounding a seaweed extract, a kava extract and a fennel extract according to the mass ratio of 4-7:4-6:4-7 and then fermenting by a composite strain. In certain embodiments, the composite strain consists of lactobacillus plantarum CGMCC1.557 and pichia pastoris ATCC76273 according to a ratio of 3:1, and the inoculation amount is 1.5+/-0.2% of the mass of the mixture. The invention also provides a preparation method of the fermentation active substance, which comprises the following steps: S1, compounding and mixing the three extracts, and inoc