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CN-120939279-B - PDRN-containing double-network hydrogel dressing applying marine biomass resources, preparation method and application

CN120939279BCN 120939279 BCN120939279 BCN 120939279BCN-120939279-B

Abstract

The invention provides a PDRN-containing double-network hydrogel dressing applying marine biomass resources, a preparation method and application. The preparation method of the dressing comprises the following steps of S1 preparing a chitosan solution, S2 preparing a PDRN solution, S3 slowly dripping the PDRN solution into the chitosan solution, stirring to form a PDRN-chitosan mixed solution, S4 preparing an AgNPs-GSH solution, S5 slowly pouring the AgNPs-GSH solution into the PDRN-chitosan mixed solution, adding a gelatin aqueous solution, gently stirring, then adding a KCl solution, rapidly stirring, and standing to form the hydrogel dressing, wherein the PDRN-containing double-network hydrogel dressing has the dual effects of resisting inflammation, resisting bacteria and promoting healing, carrageenan and chitosan promote cell migration and regeneration, the chitosan/PDRN composite protective nucleic acid prevents enzymolysis, GSH is released by buffering Ag + , and is distributed in a matrix together with the carrageenan to provide a long-acting sterilization barrier, the PDRN anti-inflammatory repair effect is complementary with the PDRN, and the whole system realizes continuous release and biological activity maintenance.

Inventors

  • FU QIANG

Assignees

  • 中国海洋大学
  • 青岛众智生物科技有限公司

Dates

Publication Date
20260512
Application Date
20250825

Claims (7)

  1. 1. The preparation method of the PDRN-containing double-network hydrogel dressing using marine biomass resources is characterized by comprising the following steps: S1, adding chitosan into glacial acetic acid solution, heating and stirring until the chitosan is completely dissolved, and cooling to obtain chitosan solution; s2, slowly dissolving the PDRN in PBS buffer solution to obtain a PDRN solution; s3, slowly dripping the PDRN solution into the chitosan solution, and stirring to form a PDRN-chitosan mixed solution; S4, adding carrageenan into deionized water, heating and stirring until the carrageenan is dissolved to obtain a carrageenan solution, slowly adding nano silver colloid into the carrageenan solution, and uniformly stirring to obtain a mixed solution; S5, slowly pouring AgNPs-GSH solution into the PDRN-chitosan mixed solution, adding gelatin aqueous solution, gently stirring, then adding KCl solution, rapidly stirring, and standing to form hydrogel dressing; in the S1, the concentration of chitosan in the chitosan solution is 0.5-1.5 g/100 mL; In the step S2, the concentration of the PDRN in the PDRN solution is 0.1-0.5 g/100 mL; In the step S3, the mixing volume ratio of the PDRN solution to the chitosan solution is 1:1-1.5.
  2. 2. The method for preparing a dual-network hydrogel dressing containing PDRN using marine biomass resources according to claim 1, wherein the concentration of carrageenan in the carrageenan solution is 0.1-1.0 g/100 mL.
  3. 3. The method for preparing the PDRN-containing double-network hydrogel dressing by using marine biomass resources according to claim 1, wherein the concentration of nano silver colloid in AgNPs-GSH solution is 5-25 ppm, the particle size of nano silver is 10-50 nm, and the addition amount of glutathione is 0.05-0.2 g/100 mL.
  4. 4. The method for preparing the PDRN-containing dual-network hydrogel dressing by using marine biomass resources according to claim 1, wherein the volume ratio of the AgNPs-GSH solution to the PDRN-chitosan mixed solution in S5 is 1:1-2.
  5. 5. The dual-network hydrogel dressing containing PDRN using marine biomass resources prepared by the method for preparing the dual-network hydrogel dressing containing PDRN using marine biomass resources according to any one of claims 1 to 4.
  6. 6. The use of a dual network hydrogel dressing comprising PDRN using marine biomass resources according to claim 5 for the preparation of a wound repair product.
  7. 7. The use of the dual network hydrogel dressing comprising PDRN using marine biomass resources according to claim 6 for the preparation of wound repair products, wherein the repair is promoting wound healing, anti-inflammatory, antibacterial, and the products include cosmetics and medicines.

Description

PDRN-containing double-network hydrogel dressing applying marine biomass resources, preparation method and application Technical Field The invention relates to the technical field of wound dressing and hydrogel, in particular to a PDRN-containing double-network hydrogel dressing applying marine biomass resources, a preparation method and application. Background Polydeoxyribonucleotide (PDRN) is a naturally derived low molecular weight DNA derivative having a molecular weight between 50kDa and 1500kDa, the most typical molecular weight of PDRN being 80kDa and 200kDa, with a gaussian distribution peak at about 132kDa. The base length of the PDRN is 50-2000 bp, the PDRN is a linear polymer of deoxyribonucleotides with phosphodiester bonds, the PDRN contains 50% double-stranded deoxyribonucleotides, the two polydeoxyribonucleotides are connected through hydrogen bonds between base pairs, a double-helix structure is finally formed, and the PDRN is mainly prepared by extraction and purification from sperm cells of salmon or rainbow trout. The similarity of the base composition of the PDRN and human DNA is up to 98%, and based on the biological effect, the PDRN has obvious effects on the aspects of tissue repair, wound healing, ischemia resistance, inflammation resistance and the like, and the clinical application range of the PDRN is gradually expanded. Experiments in vitro and in vivo indicate that the most relevant mechanism of action of PDRN is to act as an agonist of the adenosine A2A receptor, activating the A2A receptor, producing a variety of physiological effects. Chinese patent CN114642606A discloses a composition with skin barrier repairing function, its preparation method and application, which comprises high molecular weight hyaluronic acid or its salt 0.01% -0.5%, hydrolyzed hyaluronic acid or its salt 0.1% -1.0%, silk fibroin 0.01% -10%, polydeoxyribonucleic acid 0.05% -1%, and glass color factor 0.1% -10%. The high molecular weight hyaluronic acid or the salt thereof can target the stratum corneum of the skin, a water-moist breathable protective film is formed on the surface of the skin, and the extremely low molecular weight hydrolyzed hyaluronic acid or the salt thereof can be absorbed into the dermis layer of the skin in a transdermal way, so that the effect of stimulating angiogenesis is exerted. PDRN, silk fibroin and vitronectin promote collagen synthesis and cell migration. The components are matched in a synergistic way, so that the composition has an outstanding effect of promoting wound healing, can repair damaged skin barriers, and achieves the effect of rapidly repairing skin wounds. Chinese patent CN112932988a discloses an anti-aging composition containing polydeoxyribonucleic acid, including honey and polydeoxyribonucleic acid, and also discloses a skin care product, including an anti-aging composition containing polydeoxyribonucleic acid, and also including acceptable adjuvants in cosmetics. The anti-aging composition and the skin care product comprising the anti-aging composition enhance the effects of promoting skin cell regeneration and resisting aging through the synergistic effect of honey and polydeoxyribonucleic acid. At present, the PDRN has the problem of poor stability, and has certain limitation when being applied to repairing products. Marine biomass, such as Carrageenan (Carrageenan), is a natural polymer substance extracted from marine organism red algae, and has wide application potential due to good biocompatibility, biodegradability and functional activity. The PDRN is used cooperatively with other marine biomasses such as carrageenan, is expected to further enhance the tissue repair capability and stability, and has wide application prospect. Disclosure of Invention The invention aims to provide a double-network hydrogel dressing containing PDRN by using marine biomass resources, a preparation method and application thereof, and the preparation method and application of the double-network hydrogel dressing can obviously improve the stability of the PDRN and show a synergistic effect in repairing by realizing chitosan/carrageenan double-network composite hydrogel and selecting reasonable component compatibility. The technical scheme of the invention is as follows: In a first aspect, the invention provides a method for preparing a PDRN-containing dual-network hydrogel dressing using marine biomass resources, comprising the steps of: S1, adding chitosan into glacial acetic acid solution, heating and stirring until the chitosan is completely dissolved, and cooling to obtain chitosan solution; s2, slowly dissolving the PDRN in PBS buffer solution at room temperature to obtain a PDRN solution; s3, slowly dripping the PDRN solution into the chitosan solution, and stirring to form a PDRN-chitosan mixed solution; S4, adding carrageenan into deionized water, heating and stirring until the carrageenan is dissolved to obtain a carrageenan solution, slowly adding nano silver col