CN-120945067-B - SNP molecular marker related to average daily gain character of Ningxiang pigs and application thereof

CN120945067BCN 120945067 BCN120945067 BCN 120945067BCN-120945067-B

Abstract

The invention relates to the technical field of biology, in particular to an SNP molecular marker related to average daily gain characteristics of Ningxiang pigs and application thereof. The molecular markers comprise SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 12624189 site from the 5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or A, and the SNP2 molecular marker corresponds to a 13057273 site from the 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or A. The invention obtains the molecular marker obviously related to the average daily gain of the Ningxiang pigs, provides guidance for breeding of the Ningxiang pigs by using the molecular marker, can accurately and efficiently predict the average daily gain value and parting, identify and screen the pig species with high average daily gain value, and improve the economic benefit.

Inventors

LI FENGNA
DENG XIAOXIAO
GAO JINGXIA
GUO QIUPING
XU KANG
SUN JINGCHUN
YIN YULONG

Assignees

中国科学院亚热带农业生态研究所

Dates

Publication Date: 20260512
Application Date: 20250910

Claims (3)

1. The application of the reagent for detecting the SNP molecular markers related to the average daily gain characteristics of the Ningxiang pigs in detection or auxiliary detection of the average daily gain of the Ningxiang pigs is characterized in that the molecular markers are SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 12624189 th site from the 5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or A, and the SNP2 molecular marker corresponds to a 13057273 th site from the 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or A.
2. The application of the reagent for detecting the SNP molecular marker related to the average daily gain characteristic of the Ningxiang pigs in the early screening of the average daily gain characteristic of the Ningxiang pigs is characterized in that the molecular markers are SNP1 and SNP2, wherein the SNP1 molecular marker corresponds to a 12624189 th site from the 5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or A, and the SNP2 molecular marker corresponds to a 13057273 th site from the 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or A.
3. The use according to claim 1 or 2, wherein the reagent comprises a KASP marker system for detection of the SNP molecular marker, the primer sequences of the KASP marker system for detection of the SNP1 molecular marker are shown in SEQ ID No.7, SEQ ID No.8 and SEQ ID No.9, and the primer sequences of the KASP marker system for detection of the SNP2 molecular marker are shown in SEQ ID No.10, SEQ ID No.11 and SEQ ID No. 12.

Description

SNP molecular marker related to average daily gain character of Ningxiang pigs and application thereof Technical Field The invention relates to the technical field of biology, in particular to an SNP molecular marker related to average daily gain characteristics of Ningxiang pigs and application thereof. Background The pig industry in China develops rapidly, and has become a large country for pork production and pork consumption in the world. However, china is not a strong country for raising pigs, and the whole level of the pig industry is greatly different from that of the international developed country, so that the improvement of the production performance and the whole economic benefit of pigs are the struggle targets of raising enterprises and livestock raising workers. Average daily gain (AVERAGE DAILY GAIN, ADG) is an important index for measuring the growth rate of pigs, directly influences the utilization efficiency of feed, and is also commonly used as an important index for breeding together with properties such as lean meat percentage, backfat thickness and the like in breeding. Research shows that the average daily gain can be improved, so that the feeding time can be reduced, and the feeding cost can be reduced. Pig breeds with high average daily gain generally have a higher lean growth potential and feed conversion efficiency, which makes them more advantageous in commercial farming. At present, searching candidate genes related to average daily gain and developing molecular markers have become important strategies for reasonably improving economic benefits of pig farms. The SNP marker is used as a molecular marker which is widely applied at present, has higher genetic stability compared with the SSR molecular marker technology and the ISSR molecular marker technology, and meanwhile, the SNP marker can be applied to rapid and large-scale screening due to the variety of detection methods which are applicable, including Taqman method, mass spectrometry, chip method, sequencing method and the like, and the sequencing cost is continuously reduced along with the progress of the sequencing technology, so that more and more SNP markers of species are excavated and applied. The Chinese patent publication No. CN 119614716A discloses a SNP molecular marker related to the daily gain character of pigs and application thereof, but the SNP molecular marker related to the ADG character of Ningxiang pigs is not disclosed in the prior art, and the Duroc pigs and the Ningxiang pigs are two pig species with obvious differences, and the characters are influenced by a plurality of sites although the characters are the same, so that the Duroc pigs (lean meat type) and the Ningxiang pigs (fat type) have obvious differences in the SNP molecular markers related to the ADG characters. Disclosure of Invention Aiming at the defects in the prior art, the invention aims to provide an SNP molecular marker related to the average daily gain characteristic of Ningxiang pigs and application thereof. The marker can assist in identifying or screening individuals with high ADG of Ningxiang pigs for breeding, can gradually improve the growth speed and the feed utilization efficiency of the whole pig group, and increases the economic benefit. In order to achieve the aim of the invention, the invention adopts the following technical scheme: In the first aspect, a SNP molecular marker related to the average daily gain trait of Ningxiang pigs is provided, wherein the molecular marker comprises SNP1 and SNP2, the sequence of nucleic acid containing the SNP1 molecular marker is shown as SEQ ID No.1, the SNP1 molecular marker corresponds to 12624189 locus from the 5 'end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is C or A, the sequence of nucleic acid containing the SNP2 molecular marker is shown as SEQ ID No.2, and the SNP2 molecular marker corresponds to 13057273 locus from the 5' end on chromosome 1 of which the reference genome is Sus Scrofa Build11.1, and is G or A. In a second aspect, the application of the SNP molecular marker in detecting or assisting in detecting average daily gain of Ningxiang pigs is provided. In a third aspect, the application of the SNP molecular marker in screening of average daily gain early stages of Ningxiang pigs is provided. In a fourth aspect, the application of the SNP molecular marker in Ningxiang pig molecular marker assisted breeding is provided. In a fifth aspect, the application of the SNP molecular marker in Ningxiang pig breed screening is provided. Further, there is a linkage effect at both sites of SNP1 and SNP2, and the genotype combination of CA/GA is a favorable genotype. The beneficial effects of the invention are as follows: (1) The invention obtains the molecular marker obviously related to the average daily gain of the Ningxiang pigs, provides guidance for breeding of the Ningxiang pigs by using the molecular marker, can accurately and efficiently pre