CN-120965823-B - Antibacterial peptide prepared from copper-carrying lactobacillus plantarum

Abstract

The invention provides an antibacterial peptide prepared by utilizing copper-carrying lactobacillus plantarum, which is prepared by inoculating high-load copper duck-source lactobacillus plantarum into a culture medium for fermentation and then using fermentation liquor, wherein the preservation number of the copper-carrying duck-source lactobacillus plantarum is CGMCC NO.32835. The lactobacillus plantarum obtained by domestication can tolerate high-concentration copper ions, the lactobacillus plantarum obtained by domestication and breeding can stably grow, and metazoans produced by fermentation of the lactobacillus plantarum contain small peptides, small molecular organic acids, digestive enzymes and the like. As the antibacterial peptide is rich in the metazoan and has obvious inhibition effect on aquatic bird harmful bacteria, the prepared antibacterial peptide preparation effectively improves antibacterial activity, and is beneficial to eliminating intestinal inflammatory factors of poultry as a feed additive, maintaining intestinal health, improving the utilization rate of feed and improving the product quality.

Inventors

• WANG BAOWEI
• Lai Xuexiang
• WANG BINGHAN
• ZHANG MINGAI
• FAN WENLEI
• CHENG QUN
• ZHANG BEIBEI
• KONG MIN
• YAN HUIHUI

Assignees

• 青岛农业大学
• 青岛慧和生物科技有限公司

Dates

Publication Date

20260505

Application Date

20250731

Claims (4)

1. 1. The antibacterial peptide is characterized by being prepared from a metagen preparation of Lactobacillus plantarum with a preservation number of CGMCC No.32835, wherein the amino acid sequence of the antibacterial peptide is SEQ ID NO.1, SEQ ID NO.3 or SEQ ID NO. 4.
2. 2. Use of the antimicrobial peptide of claim 1 for the preparation of an antimicrobial preparation for antagonizing riemerella anatipestifer, salmonella or escherichia coli.
3. 3. An antibacterial product is characterized in that, the antibacterial product contains the antibacterial peptide as claimed in claim 1.
4. 4. The antimicrobial article of claim 3, wherein the antimicrobial article is a feed additive.

Description

Antibacterial peptide prepared from copper-carrying lactobacillus plantarum Technical Field The invention belongs to the technical field of preparation of bioactive products, and particularly relates to an antibacterial peptide prepared by using copper-loaded lactobacillus plantarum. Background Lactobacillus plantarum (Lactobacillus plantarum) is a probiotic widely applied to the fields of medicine, food, animal husbandry and the like. Many studies have shown that lactobacillus plantarum can produce probiotic effects on the host by maintaining balance of intestinal flora, reducing intestinal inflammatory factors, promoting digestion and absorption of nutrients, improving metabolism of intestinal microorganisms, and the like. Lactobacillus plantarum and metabolic products (metazoan) have the functions of inhibiting harmful bacteria, maintaining intestinal health, promoting growth and development and the like, so that the lactobacillus plantarum and metabolic products (metazoan) are used as probiotic preparations for livestock and poultry production. In addition, the lactobacillus plantarum live bacteria preparation is easily affected by various physical and chemical factors to cause the phenomena of flatulence and inactivation in the processes of preservation, preparation and transportation. The phenomenon often causes that the effect of adding the probiotics into the diet is not obvious enough. Therefore, the specific strain is selected to prepare the high-temperature-resistant high-activity metazoan and research and develop a novel microecological preparation, and the method has important economic value and market prospect for the development of the poultry industry. And pathogenic microorganism infection in poultry farming is one of the important factors causing the diseased and dead poultry. In the past, most people choose to treat pathogenic microorganism infection by antibiotics, however, bacterial drug resistance caused by abuse of antibiotics brings new threat to food safety and public health of human beings. The antibacterial peptide is a small molecular polypeptide, has broad-spectrum resistance to pathogenic microorganisms, can quickly resist invasion of pathogens, and is an important component in innate immunity. Thus, antimicrobial peptides are currently a powerful alternative to antibiotics. Disclosure of Invention The invention aims to provide an antibacterial peptide prepared by using copper-carrying lactobacillus plantarum, and the prepared antibacterial peptide has an antibacterial effect and can be used as a feed additive for poultry. The invention firstly provides an antibacterial peptide which is prepared from a metagen preparation of copper duck source lactobacillus plantarum (Lactobacillus plantarum) with the preservation number of CGMCC No. 32835; furthermore, the amino acid sequence of the antibacterial peptide is as follows: VLTLGGGALIRHWF (SEQ ID NO:1)、ATTGAIALGATAAKA (SEQ ID NO:2)、SLLLSTALLPMLSGKA (SEQ ID NO:3)、TAVGLVACSKQSANQQ (SEQ ID NO:4); the invention provides an application of the antibacterial peptide, which is an application for preparing antibacterial products; The antibacterial product is used for antagonizing disease microorganisms in common meat duck breeding such as riemerella anatipestifer, salmonella or escherichia coli; The antimicrobial product is preferably a feed additive. The invention also provides a polypeptide composition, which comprises one or more polypeptides with the sequences of SEQ ID NO. 1-4. Furthermore, the polypeptide composition also comprises a polypeptide with a sequence AVGLLLATGQAANA (SEQ ID NO: 5). The antibacterial peptide provided by the invention is obtained by separating and purifying domesticated duck-origin lactobacillus plantarum metazoan, and has obvious antibacterial activity on common duck harmful bacteria (riemerella anatipestifer, salmonella and escherichia coli). Drawings FIG. 1 shows a bacteriostasis circle diagram of mixed peptide of duck-origin lactobacillus plantarum on escherichia coli and staphylococcus aureus before and after domestication and breeding; FIG. 2 is a graph showing the inhibitory effects of mixed peptides of duck-origin lactobacillus plantarum on Li Moshi bacillus, escherichia coli and salmonella before and after domestication and breeding; FIG. 3 is a mixed peptide amino acid sequence diagram of the duck-origin lactobacillus plantarum after domestication and breeding; FIG. 4 is a diagram showing a mixed peptide structure generated by domesticated and bred duck-origin lactobacillus plantarum; FIG. 5 is a secondary mass spectrum of the amino acid sequence (SEQ ID NO: 1); FIG. 6 is a secondary mass spectrum of the amino acid sequence (SEQ ID NO: 2); FIG. 7 is a secondary mass spectrum of the amino acid sequence (SEQ ID NO: 3); FIG. 8 is a secondary mass spectrum of the amino acid sequence (SEQ ID NO: 4); FIG. 9 is a 3D structure diagram of an amino acid sequence (SEQ ID NOS: 1-4). Detailed Description The inve