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CN-120988150-B - Preparation method and application of jujube polysaccharide

CN120988150BCN 120988150 BCN120988150 BCN 120988150BCN-120988150-B

Abstract

The present disclosure provides a method for preparing jujube polysaccharide and application thereof. The present disclosure provides extraction and separation processes of Wangzizizania polysaccharide, which obtain high content polysaccharide with blood lipid reducing effect, in vivo fat and local fat or fat ratio reducing effect, and liver injury preventing, treating, improving or relieving effects.

Inventors

  • CHEN CHEN
  • LIU FENGQIN
  • LAN WEI
  • DUAN YONGBO
  • WANG RU
  • XUE TAO
  • BO CHEN
  • ZHU YANFANG
  • XUE JIANPING

Assignees

  • 淮北师范大学

Dates

Publication Date
20260512
Application Date
20250703

Claims (20)

  1. 1. A preparation method of Wangzaozi polysaccharide comprises the following steps: 1) Mixing the dried powder obtained by crushing the jujube leaves with water, and extracting by using an ultrasonic extraction method to obtain an extracting solution; 2) Concentrating the extract, mixing with absolute ethyl alcohol, standing and centrifuging to obtain a precipitate; 3) Dissolving the precipitate with water, filtering with ceramic membrane, and collecting the permeate; 4) Filtering the permeate liquid by using an organic film, collecting liquid, and drying to obtain the fructus zizyphi spinosae polysaccharide; the mass volume ratio of the crushed dry powder in the step 1) to the water is 1g (5-200 mL); The ultrasonic power of the ultrasonic extraction method in the step 1) is 200-1500W; the ultrasonic time of the ultrasonic extraction method in the step 1) is 45-105 min; the extraction temperature of the ultrasonic extraction method in the step 1) is 42-47 ℃; The pore diameter of the ceramic membrane in the step 3) is 0.1-1 mu m; The flow rate of the ceramic membrane in the step 3) is 150-200 mL/min; The interception capacity of the organic film in the step 4) is 5000-50000 daltons; The flow rate of the organic film in the step 4) is 50-250 mL/min.
  2. 2. The process according to claim 1, wherein the fresh leaves of the Wangzao jujube are dried before the crushing in step 1) to obtain dried leaves of the Wangzao jujube, and/or, The particle size of the crushed dry powder in the step 1) is 5-100 mu m, and/or, The mass volume ratio of the crushed dry powder in the step 1) to the water is 1g (100-200 mL), and/or, The ultrasonic power of the ultrasonic extraction method in the step 1) is 900-1500W and/or, The ultrasonic time of the ultrasonic extraction method in the step 1) is 45min, and/or, The extraction temperature of the ultrasonic extraction method in the step 1) is 45 ℃.
  3. 3. The process according to claim 2, wherein the pulverized dry powder in step 1) has a particle size of 5 to 50. Mu.m, The mass volume ratio of the crushed dry powder and water in the step 1) is 1g (125-200 mL), and/or, The ultrasonic power of the ultrasonic extraction method in the step 1) is 900W.
  4. 4. The process according to claim 3, wherein the pulverized dry powder in step 1) has a particle size of 5 to 10. Mu.m, The mass volume ratio of the crushed dry powder to the water in the step 1) is 1g to 150 mL.
  5. 5. The process according to claim 1, wherein the volume ratio of the extract to absolute ethanol in the step 2) is 1 (3-10), and/or, The standing time in the step 2) is 12-48 h, and/or, The centrifugation time in the step 2) is 5-30 min, and/or, And in the step 2), the centrifugal speed is 10000-20000 revolutions/min.
  6. 6. The process according to claim 5, wherein the volume ratio of the extract to absolute ethanol in the step 2) is 1 (3-5), and/or, Said resting time in said step 2) is 24 hours, and/or, And 2) centrifuging for 10-20 min.
  7. 7. The method according to claim 6, wherein the centrifugation time in the step 2) is 15 minutes.
  8. 8. The preparation method according to claim 1, wherein the mass ratio of the precipitate to water in the step 3) is 1 (1-20), and/or, The pore diameter of the ceramic membrane in the step 3) is 0.2-0.5 mu m, and/or, The flow rate of the ceramic membrane in the step 3) is 150mL/min.
  9. 9. The preparation method according to claim 8, wherein the mass ratio of the precipitate to water in the step 3) is 1 (1-10), and/or, The pore size of the ceramic membrane in the step 3) is 0.2 μm.
  10. 10. The preparation method according to claim 9, wherein the mass ratio of the precipitate to water in the step 3) is 1 (1-5).
  11. 11. The process according to claim 1, wherein the organic film in the step 4) has a retention capacity of 10000 to 30000 daltons and/or, The flow rate of the organic film in the step 4) is 100-150 mL/min.
  12. 12. The process according to claim 11, wherein the organic film in step 4) has a retention capacity of 10000 Dalton and/or, The flow rate of the organic film in the step 4) is 100 mL/min.
  13. 13. The process according to any one of claims 1 to 12, wherein the drying comprises one or more of freeze drying, hot air drying, spray drying, steam drying, vacuum drying, microwave drying or vacuum microwave drying.
  14. 14. The process according to claim 13, wherein the freeze-dried cold trap temperature is-60 to-50 ℃ and the vacuum degree is 0.1 to 1mpa, and/or, The temperature of the hot air drying is 40-60 ℃ and/or, The temperature of the vacuum microwave drying is 40-50 ℃, and the microwave power is 800-1200W.
  15. 15. The process according to claim 14, wherein the freeze-dried cold trap temperature is-55℃and the vacuum degree is 0.2MPa, and/or, The temperature of the hot air drying is 50 ℃, and/or, The temperature of the vacuum microwave drying is 45 ℃, and the microwave power is 1000W.
  16. 16. The date palm polysaccharide prepared by the preparation method according to any one of claims 1 to 15.
  17. 17. Use of the date palm polysaccharide of claim 16 in the manufacture of a medicament for preventing or treating dyslipidemia, obesity, and/or improving lipid metabolism.
  18. 18. Use of the date palm polysaccharide of claim 16 for the manufacture of a medicament for ameliorating dyslipidemia, obesity, and/or ameliorating lipid metabolism.
  19. 19. Use of the date palm polysaccharide of claim 16 for the manufacture of a medicament for the control of dyslipidemia, obesity, and/or for improving lipid metabolism.
  20. 20. Use of the date palm polysaccharide of claim 16 for the manufacture of a medicament for reducing dyslipidemia, obesity, and/or improving lipid metabolism.

Description

Preparation method and application of jujube polysaccharide Technical Field The invention belongs to the technical field of food processing, and particularly relates to a preparation method and application of date seed polysaccharide. Background Fructus Jujubae (Isodon amethystoides (Benth) Cy Wu etHsuan) is a plant of Labiatae (Labiatae) genus Rabdosia (Isodon), mainly grows in stone mountain shadow of North America, has antibacterial, antiinflammatory and antitumor effects, and comprises diterpenes, triterpenes and flavonoids. It is used for treating malignant boil, tumor, snake bite, common cold, prostatitis, acute infectious hepatitis, and gynecological inflammation. However, the physiological effect of polysaccharide of fructus Ziziphi Spinosae is not reported. The deep processing method of the fructus zizaniae caduciflorae needs further development, and the medicinal value or the health care value of the fructus zizaniae caduciflorae extract still needs deep excavation. Plant polysaccharides are encapsulated in tough plant cell walls, and conventional extraction methods (mechanical disruption, heat treatment, acid/base treatment, enzymolysis) are difficult to extract the polysaccharide, and the plant often contains a large amount of impurities coexisting with the polysaccharide, such as proteins, lipids, pigments, inorganic salts, monosaccharides/oligosaccharides, nucleic acids, small molecule metabolites and the like, and are difficult to completely remove, especially proteins or pigments closely combined with the polysaccharide, resulting in low purity of the final product. Meanwhile, the traditional separation method (precipitation, dialysis, ion exchange and gel filtration) has limited resolution, small treatment capacity and long time consumption. Therefore, it is necessary to efficiently and selectively destroy these physical barriers to release polysaccharide, and to avoid degradation of polysaccharide or massive dissolution of impurities due to excessive destruction, and to select a novel separation and purification method to obtain high purity plant polysaccharide. Currently, the prevalence of metabolic diseases such as obesity, hyperlipidemia, fatty liver and the like is increasing. Since abnormal metabolism or operation of fat causes plasma to contain one or more lipids higher than normal called hyperlipidemia, lipid-insoluble or slightly water-soluble must exist in the form of lipoproteins in combination with proteins, and thus hyperlipidemia is often hyperlipoproteinemia (hyperlipoproteinemia) which is manifested as hypercholesterolemia, hypertriglyceridemia, or both. The incidence of hyperlipidemia is an important public health problem, and its incidence has been increasing at a striking rate. It can significantly increase its risk of developing cardiovascular disease, such as atherosclerosis, which is also one of the most common causes of morbidity and mortality worldwide. Lipid lowering drugs, such as statins, are currently the most common and widely used method for treating hyperlipidemia, but due to their high price and side effects of these drugs, research is beginning to develop more effective and safer alternatives or auxiliary products for lowering blood lipid. Disclosure of Invention In order to solve at least one of the problems, the present disclosure provides a preparation method and application of a date seed polysaccharide. According to one aspect of the present disclosure, there is provided a method for preparing a date seed polysaccharide, comprising the steps of: The invention provides a preparation method of jujube polysaccharide, which comprises the following steps: 1) Mixing the dried powder obtained by crushing the jujube leaves with water, and extracting by using an ultrasonic extraction method to obtain an extracting solution; 2) Concentrating the extract, mixing with absolute ethyl alcohol, standing and centrifuging to obtain a precipitate; 3) Dissolving the precipitate with water, filtering with ceramic membrane, and collecting the permeate; 4) Filtering the permeate liquid by using an organic film, collecting liquid, and drying to obtain the fructus zizaniae caduciflorae polysaccharide. In some embodiments, the fresh jujube leaves are dried before the pulverizing in step 1), resulting in dried jujube leaves. In some embodiments, the drying is to a constant weight. It will be appreciated by those skilled in the art that any known comminution method may be used, non-limiting examples of comminution include mechanical impact comminution, jet milling, ball or vibration milling comminution, high pressure comminution, cryogenic comminution, ultra micro comminution, nano comminution, and the like, all as routine options for those skilled in the art. In some embodiments, the particle size of the pulverized dry powder in step 1) is 5 to 100 μm, preferably 5 to 50 μm, more preferably 5 to 10 μm. In some embodiments, the particle size of the pulverized dry powder in step 1