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CN-120992289-B - Seed cut sample female parent and sample corresponding type collection coding system

CN120992289BCN 120992289 BCN120992289 BCN 120992289BCN-120992289-B

Abstract

The invention relates to the technical field of breeding, in particular to a seed sample cutting female parent and sample corresponding collecting and encoding system which comprises a motion control system, a counter and a cutting completion signal module, wherein the motion control system is electrically connected with a first motion module and a second motion module, a first grid position for controlling a sample box supported by the first motion module and a first grid position for controlling a collection box supported by the second motion module to be located under an initial collecting position is controlled, the value of the counter is set to be N, the initial value of the counter is set to be 1, and the value N of the counter is increased by 1 after a driving instruction is generated according to the cutting completion signal each time. The invention completes synchronous linkage driving and accurate site coding of the sample box and the female parent collecting box by arranging the synchronous motion control system and the counter, achieves the technical effect that the sample and the female parent automatically fall into the physical grids with the same serial numbers after successful cutting at one time, and achieves the technical effect of establishing the traceable electronic file by integrating the database and dynamically correlating with the serial numbers of the counter.

Inventors

  • LU HONG
  • DU GUOHUA
  • ZHU ZHENGHANG
  • YIN YILIANG

Assignees

  • 中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心)

Dates

Publication Date
20260505
Application Date
20250827

Claims (7)

  1. 1. The seed sample cutting female parent and sample corresponding collecting and encoding system is applied to laser sample cutting equipment, the equipment comprises a sample collecting group (200) for collecting samples and a body collecting group (300) for collecting female parent, the sample collecting group (200) comprises a first motion module (220) and a plurality of sample boxes (210), and the body collecting group (300) comprises a second motion module (320) and a plurality of collecting boxes (310); the system is characterized by comprising a motion control system, a counter and a cutting completion signal module, wherein the motion control system is electrically connected with the first motion module (220) and the second motion module (320); The motion control system is configured to control the first motion module (220) to prop up the sample box (210) and control the second motion module (320) to prop up the first grid position of the collecting box (310) to be located right below an initial collecting position, set a value of a counter to be N with an initial value of 1, and increase the value N of the counter by 1 after generating a driving instruction according to the cutting completion signal each time, wherein the current value N of the counter indicates the sequence numbers of the current effective grids of the sample box (210) and the collecting box (310); The cutting completion signal module generates a driving instruction after laser cutting, synchronously controls the first movement module (220) and the second movement module (320) to act, and drives the collecting positions of the sample box (210) and the collecting box (310) to synchronously move to the next grid position, so that samples generated by one-time cutting and female parents are correspondingly collected in grids with the same serial numbers of the sample box (210) and the collecting box (310); the motion control system further comprises a seed discarding signal module, if a seed discarding signal is received, the response of the cutting completion signal module is restrained, and a driving instruction is not generated; The motion control system further comprises a photoelectric sensor positioned on the sample falling path and used for detecting whether a sample falls, wherein the cutting completion signal module generates a driving instruction when the photoelectric sensor detects that the sample falls; the first motion module (220) and the second motion module (320) are XY two-axis motion modules, wherein the driving instruction is used for controlling the XY two-axis motion modules to move by one lattice distance along the X axis or the Y axis.
  2. 2. The seed cut female parent and sample corresponding collection encoding system of claim 1, wherein the motion control system further comprises a database for storing records, wherein the records include a lot identifier, a grid number, seed class information, and wherein each time the counter value N is incremented, a new record associated with the current grid number N is created.
  3. 3. The seed-cut female parent-sample correspondence collection encoding system of claim 2, wherein the sample cartridges (210) and the collection cartridges (310) are multi-cell array cartridges with cells arranged in a matrix, and wherein the driving instructions are configured to control the sample collection set (200) to sequentially move all cells in a row-first or column-first order for collection.
  4. 4. The seed sample cutting female parent and sample corresponding collecting and encoding system according to claim 3, wherein the first motion module (220) and the second motion module (320) have the same structure composition, are coaxially connected with a servo motor and a screw rod, a sliding block is sleeved on the screw rod in a threaded manner, and the top surface of the sliding block is disc-shaped and used for placing a plurality of sample boxes (210) and a plurality of collecting boxes (310).
  5. 5. The seed sample cutting female parent and sample corresponding collecting and encoding system according to claim 4, wherein a turntable (100) is arranged above the first motion module (220) and the second motion module (320), a plurality of pneumatic clamping jaws (110) are installed on the outer side of the turntable (100) at equal intervals in an annular shape, and the turntable (100) intermittently rotates at a fixed angle by sending out pulses through a servo motor.
  6. 6. The seed cut female parent and sample corresponding collection encoding system of claim 5, comprising the steps of: s1, initializing a first motion module (220) and a second motion module (320) to enable an initial collection position to be located at a first grid position of a sample box (210) and a collection box (310) so as to be aligned to the position right below the rest position of a pneumatic clamping jaw (110); S2, when the photoelectric sensor detects that a sample falls, the first motion module (220) and the second motion module (320) are synchronously driven by responding to the cutting completion signal module, so that the collection positions of the sample box (210) and the collection box (310) synchronously move to the next grid; s3, collecting the sample and the female parent generated by one-time cutting in grids aligned with the sample box (210) and the collecting box (310) before moving.
  7. 7. The system of claim 6, wherein in step S2, the initial value of the counter N is 1, the value of N is increased by 1 after each synchronous driving, wherein in step S3, the sample and the female parent are collected in N-numbered grids, and the collection bits are aligned to N+1 grids after driving.

Description

Seed cut sample female parent and sample corresponding type collection coding system Technical Field The invention relates to the technical field of breeding, in particular to a seed sample cutting female parent and sample corresponding collecting and encoding system. Background In the high throughput gene analysis process of molecular breeding, a large number of seeds need to be sliced and sampled, and each cut sample can be ensured to accurately correspond to the maternal seed thereof, so that the accuracy and traceability of subsequent gene data are ensured. The invention patent with the application number of CN202011616909.1 discloses a biological breeding slicer, and is fixed to current slicing equipment structure, and the section interval is inconvenient to adjust, and the commonality is relatively poor, and can't fix the position of biological seed when slicing, and stability is relatively poor, the lower problem of section efficiency, and the scheme is proposed now, and it includes the section platform, the top fixed mounting of section platform has two connecting seats, and the movable hole has all been seted up to one side of two connecting seats, and the same movable plate has been slided in two movable holes, a plurality of regulating grooves have been seted up to the movable plate bottom, and equal slidable mounting has the regulating plate in a plurality of regulating grooves, and the equal fixed mounting of bottom of a plurality of regulating plates has the blade. The slicing machine is reasonable in structure, convenient to operate, flexible in structure, convenient to adjust slicing intervals, good in universality, capable of meeting different slicing requirements, good in stability and high in slicing efficiency, and the position of biological seeds can be stabilized while slicing is carried out. The prior art focuses mainly on solving the problems of slice physical operations per se, such as adjusting slice spacing, fixing seed position to improve slice efficiency and stability. However, such devices suffer from a significant drawback in that they lack the ability to automate, high reliability correspondence management of post-sectioning samples with the female parent. At present, the correspondence of a sample and a female parent depends on manual marking, split charging and recording, and the mode is low in efficiency and easy to confuse in a large-batch sample cutting scene. Once the corresponding relation between the sample and the female parent is lost or wrong, the experimental data of the whole batch is lost, so that serious time and resource waste is caused. Therefore, there is a strong need in the art for a code collection system that can be integrated into an automated sample cutting apparatus and that can automatically and without error establish and record the correspondence between the sample and the female parent. Disclosure of Invention In order to overcome the defects in the prior art, the invention aims to provide a seed sample cutting female parent and sample corresponding collecting and encoding system, which ensures that samples cut from the same seed and the remaining female parent are automatically and erroneously collected into a collecting box grid with a unique corresponding relation by introducing an encoding control logic and a position synchronization mechanism on the basis of original laser equipment and generates a traceable encoding record so as to solve the problems in the prior art. In order to achieve the above purpose, the invention provides a seed sample cutting female parent and sample corresponding collecting and encoding system, which is applied to a laser sample cutting device, wherein the device comprises a sample collecting group for collecting samples and a body collecting group for collecting female parent, the sample collecting group comprises a first motion module and a plurality of sample boxes, and the body collecting group comprises a second motion module and a plurality of collecting boxes; the system comprises a motion control system, a counter and a cutting completion signal module, wherein the motion control system is electrically connected with the first motion module and the second motion module; The motion control system is configured to control the first motion module to prop up the sample box and control the second motion module to prop up the first grid position of the collecting box to be located right below an initial collecting position, set the numerical value of a counter to be N, and set the numerical value of the counter to be 1, and increase the numerical value of the counter by 1 after generating a driving instruction according to the cutting completion signal each time, wherein the current numerical value N of the counter indicates the serial numbers of the current effective grids of the sample box and the collecting box; The cutting completion signal module generates a driving instruction after laser cutting, synchronou