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CN-121015954-B - Antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing and preparation method thereof

CN121015954BCN 121015954 BCN121015954 BCN 121015954BCN-121015954-B

Abstract

The invention discloses an antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing and a preparation method thereof, and belongs to the technical field of hydrogel dressing preparation. The antibacterial hollow mesoporous silica sphere injectable hydrogel dressing provided by the invention is prepared by self-crosslinking a four-arm polyethylene glycol with a terminal group modified by o-phthalaldehyde serving as a crosslinking agent with a hollow mesoporous silica sphere coated with tannic acid in a solvent. Compared with the prior art, the antibacterial hollow mesoporous silica sphere injectable hydrogel dressing provided by the invention can be gelled without the participation of catalase, has better gelling time, can not be gelled too slowly or too quickly, can be molded in situ by injection, can treat deep wounds (the existing hydrogel can be gelled too quickly or too slowly and can only be directly made into gel for use on shallow wounds), and can improve the mechanical strength of the gel, and also has good biocompatibility, degradability, antibacterial property and repair promoting capability.

Inventors

  • MEI LIN
  • YAO YIFAN
  • LI SITONG
  • LUO RAN

Assignees

  • 中国医学科学院生物医学工程研究所

Dates

Publication Date
20260508
Application Date
20250829

Claims (10)

  1. 1. The preparation method of the antibacterial hollow mesoporous silica sphere injectable hydrogel dressing is characterized by comprising the following steps of: dispersing the hollow mesoporous silica spheres in water, mixing with a tannic acid solution, and collecting to obtain hollow mesoporous silica spheres coated with tannic acid; dissolving four-arm polyethylene glycol capped by o-phthalaldehyde in a solvent to obtain a solution A; dissolving the hollow mesoporous silica spheres coated with tannic acid in a solvent to obtain a solution B; Ciprofloxacin hydrochloride is dissolved in a solvent to obtain a solution C; mixing the solution A, the solution B and the solution C and then reacting to obtain the antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing; The structural formula of the phthalic dialdehyde end-capped four-arm polyethylene glycol is as follows: wherein n is the polymerization degree, and the value range is more than or equal to 25 and less than or equal to 100; In the preparation process, the hollow mesoporous silica sphere and the four-arm polyethylene glycol capped by the phthalic dialdehyde are crosslinked by themselves, and the aldehyde group of the phthalic dialdehyde at the tail end of the polyethylene glycol reacts with the amino group on the hollow mesoporous silica sphere to generate the nitrogen-containing five-membered heterocycle.
  2. 2. The preparation method of claim 1, wherein the entrapment rate of the hollow mesoporous silica spheres entrapping tannic acid is 3.5-4wt%.
  3. 3. The method of claim 1, wherein the solvent independently comprises one or more of water, physiological saline, buffer solution, bacterial culture solution, tissue culture solution, and body fluid.
  4. 4. The preparation method of the O-phthalaldehyde-terminated four-arm polyethylene glycol solution A according to claim 1, wherein the pH value of the solution A is 4-9, and/or the mass fraction of the O-phthalaldehyde-terminated four-arm polyethylene glycol in the solution A is 5-10%.
  5. 5. The preparation method of the tannic acid-coated hollow mesoporous silica spheres is characterized in that the pH value of the solution B is 4-9, and/or the mass fraction of the tannic acid-coated hollow mesoporous silica spheres in the solution B is 5-10%.
  6. 6. The preparation method of claim 1, wherein the mass fraction of ciprofloxacin hydrochloride in the solution C is 5-10%.
  7. 7. The preparation method of claim 1, wherein the mass ratio of the hollow mesoporous silica spheres encapsulating tannic acid, the four-arm polyethylene glycol capped by o-phthalaldehyde and the ciprofloxacin hydrochloride is 1-3:1:1.
  8. 8. The method according to claim 1, wherein the dissolution temperature is independently 10 to 60 ℃.
  9. 9. The method according to claim 1, wherein the reaction temperature is 10 to 60 ℃.
  10. 10. The antibacterial hollow mesoporous silica sphere injectable hydrogel dressing prepared by the preparation method of any one of claims 1-9.

Description

Antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing and preparation method thereof Technical Field The invention belongs to the technical field of hydrogel dressing preparation, and particularly relates to an antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing and a preparation method thereof. Background In clinical care of complicated wound surfaces such as chronic wound infection, burn, deep wound and the like, the traditional dressing generally has the bottleneck of insufficient antibacterial performance, difficulty in attaching irregular wound surfaces, secondary damage caused by dressing change, incapability of providing active repair factors and the like, so that the injectable hydrogel dressing becomes a research hotspot due to good in-situ forming capability, excellent tissue compatibility and three-dimensional micropore structure, can fill wound cavities of any shape and maintain a moist environment, but the existing product still faces the challenges of weak mechanical strength, single function and lack of a long-acting antibacterial mechanism, especially has poor effect on resisting multiple drug-resistant bacteria, most systems can be blocked only by animal theory, cannot realize synergy of antibacterial and repair-promoting functions, and has the problems of easy burst release, high biological toxicity and poor stability due to high specific surface area, adjustable aperture and good biological compatibility, but the prior art fails to show potential in the controlled release field as a drug carrier, and has the advantages of integrating the hollow mesoporous silica sphere with the injectable hydrogel, namely the rapid in-situ controllable gelation, rapid regeneration of the injectable hydrogel, rapid in-situ gelation-promoting and the active repair-promoting functions. In addition, the existing hydrogel can be glued too quickly or too slowly, and can only be directly made into gel for use, and is used for superficial wounds. Therefore, the hydrogel dressing which has better gel forming time, can not form gel too slowly or too quickly, can be molded in situ by injection and can treat deep wounds is of great significance. Disclosure of Invention The invention aims to provide an antibacterial hollow mesoporous silica sphere injectable hydrogel dressing and a preparation method thereof, which are used for solving the problems existing in the prior art, the antibacterial hollow mesoporous silica sphere injectable hydrogel dressing provided by the invention can be glued without the participation of catalase, has higher glue-forming strength, has injectability and has good repair promoting effect on deep wounds. In order to achieve the above object, the present invention provides the following solutions: the invention provides a preparation method of an antibacterial hollow mesoporous silica sphere injectable hydrogel dressing, which comprises the following steps: dispersing the hollow mesoporous silica spheres in water, mixing with a tannic acid solution, and collecting to obtain hollow mesoporous silica spheres coated with tannic acid; dissolving four-arm polyethylene glycol capped by o-phthalaldehyde in a solvent to obtain a solution A; dissolving the hollow mesoporous silica spheres coated with tannic acid in a solvent to obtain a solution B; Ciprofloxacin hydrochloride is dissolved in a solvent to obtain a solution C; mixing the solution A, the solution B and the solution C and then reacting to obtain the antibacterial hollow mesoporous silicon sphere injectable hydrogel dressing; The structural formula of the phthalic dialdehyde end-capped four-arm polyethylene glycol is as follows: Wherein n is the polymerization degree, and the value range is more than or equal to 25 and less than or equal to 100. If the polymerization degree is too low or too high, the gel forming time and the gel forming mechanical strength of the hydrogel dressing are adversely affected, and the ideal gel forming time and high mechanical strength can be obtained only by controlling the polymerization degree in the range, because the too low polymerization degree can lead to the prolongation of the gel forming time of the hydrogel, the difficulty of gel forming, the poor mechanical strength and the easy disintegration, and the too high polymerization degree leads to the too fast gel forming (poor operability), but the material becomes brittle and the toughness is reduced. Therefore, the moderate polymerization degree can realize controllable gel formation and strength and toughness balance, and is an ideal choice for the design of the hydrogel dressing. The source of the phthalic-dialdehyde-terminated four-arm polyethylene glycol is not particularly limited, and the phthalic-dialdehyde-terminated four-arm polyethylene glycol is a common commercial product or is prepared according to a preparation method known in the art. Preferably, the entrapment rate of the hollow mesoporous