CN-121040346-B - Microorganism synergistic plant planting method in heavy metal contaminated soil

CN121040346BCN 121040346 BCN121040346 BCN 121040346BCN-121040346-B

Abstract

The invention belongs to the field of agricultural planting, and particularly relates to a plant planting method by microorganism cooperation in heavy metal contaminated soil. The planting method comprises the steps of planting plants mainly comprising crops in heavy metal contaminated soil to be repaired, applying 2-keto-L-gulonic acid mother liquor, plant growth promoting bacteria and plant biological hormone to periapical soil and/or leaves of the plants in a vegetative growth stage of the plants, and applying gulonic acid mother liquor and plant endophyte bacillus to periapical soil and/or leaves of the plants in a reproductive growth stage of the plants. The invention applies 2-keto-L-gulonic acid mother liquor, plant growth promoting bacteria and plant biological hormone in the vegetative growth stage of crops, and applies 2-keto-L-gulonic acid mother liquor and plant endophytic bacillus in the reproductive growth stage, thereby not only greatly improving the heavy metal enrichment efficiency of roots, stems and leaves of crops, but also greatly reducing the heavy metal concentration of crops seeds and meeting the national feed or food standard.

Inventors

XU HUI
YANG WEICHAO
SUN HAO
GAO MINGFU
CHENG HAOTIAN
KONG SHUANG
LV XIAOHUAN

Assignees

中国科学院沈阳应用生态研究所

Dates

Publication Date: 20260512
Application Date: 20250710

Claims (7)

1. The method for planting the plants by the cooperation of microorganisms in the heavy metal contaminated soil is characterized by comprising the following steps of: step 1, planting plants in heavy metal contaminated soil to be repaired; Step 2, applying 2-keto-L-gulonic acid mother liquor, plant growth promoting bacteria and plant biological hormone to the periapical soil and/or leaf surfaces of the plants in the vegetative growth stage, wherein the plant biological hormone is one or more of humic acid, fulvic acid, polyglutamic acid, itaconic acid, sorbitol, citric acid and gamma-aminobutyric acid; Step 3, applying gulonic acid mother liquor and endophytic bacillus to the periroot soil and/or leaves of the plant in the reproductive growth stage, wherein the strain of the endophytic bacillus is named CBS-5 (Bacillus endophyticus CBS-5) and is preserved in China center for type culture collection of industrial microorganisms with the preservation number of CICC 10622; The plant is corn, the nutrition growth stage is a seedling emergence stage to a large horn mouth stage, and the reproduction growth stage is a male extraction stage to a mature stage of the corn.
2. The method for plant cultivation by microorganism cooperation in heavy metal contaminated soil according to claim 1, wherein the 2-keto-L-gulonic acid mother liquor is residual liquid at the bottom of a kettle after ultrafiltration, evaporation, concentration, crystallization and extraction of 2-keto-L-gulonic acid in the second step of 2-keto-L-gulonic acid fermentation liquor in vitamin C industrial fermentation.
3. The method for planting the plants by the cooperation of the microorganisms in the heavy metal contaminated soil according to claim 2 is characterized in that the 2-keto-L-gulonic acid mother solution comprises 22-30% by mass of 2-keto-L-gulonic acid, 2-4% by mass of oxalic acid, 4-9% by mass of formic acid, 1-3% by mass of acetic acid, less than 1% by mass of amino acid, polypeptide, oligosaccharide and nucleotide, and the balance of water.
4. The method for plant cultivation by microbial cooperation in heavy metal contaminated soil according to claim 1, wherein the pH value of the 2-keto-L-gulonic acid mother liquor is 2.0 to 8.0.
5. The method for plant cultivation in heavy metal contaminated soil according to claim 1, wherein said plant growth promoting bacteria is a single or composite bacterial agent of bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis) or bacillus amyloliquefaciens (Bacillus amyloliquefaciens), said bacterial agent being a liquid bacterial agent or a solid bacterial agent.
6. The plant planting method for the cooperation of microorganisms in the heavy metal contaminated soil, which is disclosed in claim 1, is characterized in that the content of heavy metals cadmium, chromium, lead, mercury and arsenic in corn rootstalks and leaves is improved by 2-4 times by the plant planting method, and the content of heavy metals cadmium, chromium, lead, mercury and arsenic in corn seeds is reduced to 1/8~3/4 of that in a control group by the plant planting method.
7. The method for the microbial synergistic plant cultivation in heavy metal contaminated soil according to claim 1, wherein the plant is wheat, rice, broad bean, soybean, millet, rape, sesame, watermelon, apple or melon.

Description

Microorganism synergistic plant planting method in heavy metal contaminated soil Technical Field The invention belongs to the field of agricultural planting, and particularly relates to a plant planting method by microorganism cooperation in heavy metal contaminated soil. Background The plant repairing technology is an emerging green environment-friendly repairing technology, and has a plurality of incomparable advantages compared with the traditional repairing technology. The phytoremediation has the advantages of low cost, no damage to the site structure, no secondary pollution, environmental friendliness, easy acceptance and the like. However, phytoremediation techniques have some drawbacks, making it difficult to achieve widespread use to date. These disadvantages include 1) longer phytoremediation cycles and low remediation efficiency. The effectiveness and rate of phytoremediation depends on the plant's biological yield per unit area and the concentration of heavy metals in the aerial parts. Super-enriched plants have the characteristics of smaller biomass, slow growth and the like although having stronger enrichment capability, while high-biomass plants have low heavy metal concentration in bodies and weak enrichment capability although the biomass of overground parts is larger, so that the repair efficiency is low. Most of the super-enriched plants at present have the defects of slow growth, small biomass, limited root system expansion depth, metal selectivity, low heavy metal transfer rate from root to overground part and the like, so that the actual repair efficiency is low and the repair time is long, 2) the repair capability of a single plant is limited, only one or two heavy metals can be generally enriched, 3) the plant repair technology is easily influenced by environmental factors, so that the repair effect is unstable, 4) the economic benefit of plant repair is low, and 5) the time for plant growth is short in northern areas with short frost-free period. In addition, plants, especially crops, are planted in heavy metal contaminated soil, and because more heavy metals are often enriched in seeds or fruits, the planted plants cannot enter a food chain or are converted into high-value products, and economic benefits are difficult to generate. These problems severely restrict the large-scale application of contaminated soil phytoremediation technology, and no substantial breakthrough has been seen since many years before the new technology in this field was studied almost stop. Disclosure of Invention The invention provides a novel plant planting method by microorganism cooperation in heavy metal contaminated soil, which can improve the heavy metal enrichment efficiency of plant roots, stems and leaves, shorten the restoration period, and reduce the heavy metal content of crop fruits/seeds (edible parts) so as to meet the feed and/or food standards, thereby generating economic benefit and reducing the restoration cost. The technical scheme adopted for solving the technical problems is as follows: A method of microbiologically synergistic plant cultivation in heavy metal contaminated soil, the method comprising the steps of: step 1, planting plants in heavy metal contaminated soil to be repaired, wherein the plants are preferably plants mainly comprising crops; step 2, applying 2-keto-L-gulonic acid mother liquor (hereinafter called gulonic acid mother liquor for short), plant growth promoting bacteria and plant biological hormone to the periroot soil and/or leaf surface of the plant in the vegetative growth stage; And 3, applying gulonic acid mother liquor and endophytic bacillus to the periroot soil and/or leaves of the plant in the reproductive growth stage of the plant. In the plant planting method with the cooperation of the microorganism in the heavy metal contaminated soil, the 2-keto-L-gulonic acid mother liquor is residual liquid (Residue After Evaporation, abbreviated as RAE) at the bottom of a kettle after ultrafiltration, evaporation, concentration, crystallization and 2-keto-L-gulonic acid extraction of the 2-keto-L-gulonic acid fermentation liquor in the second step in the industrial fermentation of vitamin C. In the plant planting method with the cooperation of the microorganism in the heavy metal contaminated soil, the main components of the 2-keto-L-gulonic acid mother solution comprise 2-keto-L-gulonic acid, a small amount of oxalic acid, formic acid, acetic acid and trace amino acids, polypeptides, oligosaccharides and nucleotides. The molecular weight of the above components is below 500 daltons, and most of them are below 200 daltons, so the gulonic acid mother liquor is also called small molecular organic carbon or low molecular organic acid. In the plant planting method with the cooperation of the microorganisms in the heavy metal contaminated soil, the pH value of the 2-keto-L-gulonic acid mother solution is adjusted to 2.0-8.0, and the pH value of the mother solution is