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CN-121065302-B - Brain polypeptide raw material with intelligence improving and brain strengthening effects and preparation method thereof

CN121065302BCN 121065302 BCN121065302 BCN 121065302BCN-121065302-B

Abstract

The invention provides a brain polypeptide raw material with the effects of improving intelligence and strengthening brain and a preparation method thereof. The preparation method comprises the steps of S1, pretreatment, S2, first hydrolysis, S4 and second treatment, wherein the pH of a prehydrolysis liquid is adjusted to 6.5-7.5, neutral protease is added, the addition amount of the neutral protease is 3.5-4.5% of the mass of bovine brain protein, enzymolysis is carried out for 1-3 hours at 50-60 ℃ to obtain a first hydrolysis liquid after enzyme deactivation, the pH of the first hydrolysis liquid is adjusted to 6.0-7.0, papain is added, the addition amount of the papain is 1.0-3.0% of the mass of bovine brain protein, enzymolysis is carried out for 1.5-2.5 hours at 45-65 ℃ to obtain a second hydrolysis liquid after enzyme deactivation, and the brain polypeptide raw material powder is obtained after the post treatment. The brain polypeptide raw material can remarkably remove DPPH free radical and ABTS free radical, inhibit the activity of acetylcholinesterase, repair PC12 nerve cells, inhibit pro-apoptosis gene Bax and promote the expression of anti-apoptosis gene Bcl-2, and play roles in developing intelligence, strengthening brain and protecting brain nerves together.

Inventors

  • HUANG JUNYANG
  • BAI GUANGHUI
  • XU HUIHUI
  • YU TIAN
  • XU MEI
  • ZHAO DAZHOU

Assignees

  • 厦门元之道生物科技有限公司

Dates

Publication Date
20260505
Application Date
20251110

Claims (6)

  1. 1. The preparation method of the brain polypeptide raw material with the functions of improving intelligence, strengthening brain and protecting brain nerves is characterized by comprising the following steps of: s1, preprocessing, namely removing part of white fat from quarantined bovine brain tissues, mincing, adding water according to a mass ratio of 1:2-1:4, mixing, and carrying out medium-temperature heat treatment at 54-56 ℃ for 0.5-1.5 h to obtain prehydrolysis liquid; s2, performing primary hydrolysis, namely adjusting the pH of the prehydrolysis liquid to 6.5-7.5, adding neutral protease with the addition amount of 3.5-4.5% of the bovine brain protein, performing enzymolysis for 1-3 hours at 50-60 ℃ and inactivating enzyme to obtain a first hydrolysis liquid, wherein the enzyme activity of the neutral protease is 2800-3600U/g; S3, performing secondary hydrolysis, namely adjusting the pH of the first hydrolysate to 6.0-7.0, adding papain, wherein the addition amount of the papain is 1.0-3.0% of the mass of bovine brain protein, and performing enzymolysis for 1.5-2.5 hours at 45-65 ℃ to obtain a second hydrolysate after enzyme deactivation, wherein the enzyme activity of the papain is 4000-12000U/g; S4, post-treatment, namely regulating the pH value of the second hydrolysate to 4.0-5.0, and carrying out centrifugation, active carbon decolorization and deodorization, diatomite filtration, ultrafiltration, nanofiltration desalination and concentration, and spray drying to obtain the brain polypeptide raw material powder, wherein the ultrafiltration interception molecular weight is less than 5 kDa, and the mass content of the concentrated brain polypeptide raw material is 10-25%.
  2. 2. The method for preparing the brain polypeptide raw material with the functions of improving intelligence, strengthening brain and protecting brain nerves according to claim 1, wherein in the step S2 and the step S3, the enzyme deactivation process is to deactivate enzymes at 84-86 ℃ for 15-20 min.
  3. 3. The preparation method of the brain polypeptide raw material with the functions of improving intelligence, strengthening brain and protecting brain nerves, which is characterized in that in the step S4, the steps of decoloring, deodorizing and filtering by diatomite by active carbon are that the second hydrolysate is cooled to 50-60 ℃, the active carbon is added for treatment for 0.5-1.5 h, and the mixture is filtered by diatomite and a 0.45 mu m water-based film after centrifugation.
  4. 4. The method for preparing a brain polypeptide raw material with the functions of improving intelligence, strengthening brain and protecting brain nerves according to claim 3, wherein the addition amount of the activated carbon is 3.0-5.0% of the mass of the bovine brain protein.
  5. 5. The method for preparing the brain polypeptide raw material with the functions of improving intelligence, strengthening brain and protecting brain nerves according to claim 3, wherein the centrifugation condition is 5000 rpm min to 10 min.
  6. 6. A brain polypeptide raw material composition with the functions of improving intelligence, strengthening brain and protecting brain nerves, which is characterized by being prepared by the method of any one of claims 1 to 5.

Description

Brain polypeptide raw material with intelligence improving and brain strengthening effects and preparation method thereof Technical Field The invention relates to a brain polypeptide raw material with the effects of improving intelligence and strengthening brain and a preparation method thereof. Background Along with the aging of population, diseases caused by neurotoxic oxidative damage are more and more increased, and cerebral nerve oxidative damage and neurodegenerative diseases are also more and more caused, for example, wherein brain polypeptide raw materials are small molecular active peptides obtained by extracting animal brain tissues (such as bovine brain or pig brain) through biological enzyme enzymolysis, and the brain polypeptide raw materials have various intelligence-improving and brain-strengthening functions. ① Promoting nerve cell survival and regeneration, promoting nerve cell neurite growth, inhibiting apoptosis signal path, ② improving brain metabolism and energy supply, enhancing mitochondrial function, improving ATP synthesis efficiency, and relieving cerebral nerve cell hypoxia oxidative damage. Chinese patent CN118726526a discloses a preparation method of brain peptide for preventing dementia, which comprises the steps of refrigerating, grinding, acid discharging, molecular resonance auxiliary salting out, molecular resonance auxiliary enzymolysis, secondary enzymolysis, ultrafiltration and drying to obtain brain peptide for preventing dementia, and the extraction rate of brain peptide is improved by adopting trypsin and chymotrypsin to cooperatively use. Chinese patent CN120365355A discloses an active peptide and brain peptide enzymolysis extract for improving memory, a preparation method and application thereof, microwave-assisted enzymolysis trypsin and pepsin enzymolysis are adopted, carbon dioxide gas is introduced in microwave treatment for aeration, small molecular peptide with higher activity is extracted, and an AAVP peptide segment with the functions of improving sleep and improving memory is screened out. The core active peptide segment with the definite functions of improving intelligence and strengthening brain in the bovine brain peptide and the action mechanism thereof still lack of systematic research, so that the development of the bovine brain peptide product with high activity and high specificity is still an important research direction at present. Meanwhile, the existing preparation of the intelligence-improving brain-strengthening peptide has the problems of complex equipment, low yield of active peptide, single function and the like. Therefore, the development of the preparation process of the intelligence-improving brain-strengthening peptide with high efficiency, low cost and comprehensive functions has important practical significance and application value. Disclosure of Invention The invention provides a brain polypeptide raw material with the effects of improving intelligence and strengthening brain and a preparation method thereof, which can effectively solve the problems. The invention provides a preparation method of a brain polypeptide raw material with the effects of improving intelligence, strengthening brain and protecting brain nerves, which comprises the following steps: s1, preprocessing, namely removing part of white fat from quarantined bovine brain tissues, mincing, adding water according to a mass ratio of 1:2-1:4, mixing, and carrying out medium-temperature heat treatment at 54-56 ℃ for 0.5-1.5 h to obtain prehydrolysis liquid; s2, performing primary hydrolysis, namely adjusting the pH of the pre-hydrolyzed solution to 6.5-7.5, adding neutral protease with the addition amount of 3.5-4.5% of the bovine brain protein, performing enzymolysis for 1-3 hours at 50-60 ℃, and inactivating enzyme to obtain a first hydrolyzed solution; S3, performing secondary hydrolysis, namely adjusting the pH value of the first hydrolysate to 6.0-7.0, adding papain, wherein the addition amount is 1.0-3.0% of the mass of bovine brain protein, performing enzymolysis for 1.5-2.5 hours at 45-65 ℃, and inactivating enzyme to obtain a second hydrolysate; S4, post-treatment, namely regulating the pH value of the second hydrolysate to 4.0-5.0, and obtaining the brain polypeptide raw material powder through centrifugation, active carbon decolorization and deodorization, diatomite filtration, ultrafiltration, nanofiltration desalination concentration and spray drying, wherein the ultrafiltration interception molecular weight is less than 5 kDa, and the mass content of the concentrated brain polypeptide raw material is 10-25%. In some embodiments, in the step S2, the enzyme activity of the neutral protease is 2800-3600U/g, and in the step S3, the enzyme activity of the papain is 4000-12000U/g. In some embodiments, in step S2 and step S3, the enzyme deactivation process is to deactivate enzymes at 84-86 ℃ for 15-20 min. In some embodiments, in the step S4, the steps of decolor