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CN-121086094-B - Active polysaccharide of ganoderma lucidum mutant strain, preparation method and application thereof

CN121086094BCN 121086094 BCN121086094 BCN 121086094BCN-121086094-B

Abstract

The invention discloses active polysaccharide of a ganoderma lucidum mutant strain, a preparation method and application thereof, and relates to the technical field of biological medicine. The preparation method comprises the step of extracting mycelium of a mutagenized ganoderma lucidum strain JLAU 210928 by a water extraction and alcohol precipitation method to prepare active polysaccharide of the ganoderma lucidum mutagenized strain, wherein the mutagenized ganoderma lucidum strain JLAU 210928 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) on 9 months and 30 days in 2021, and the preservation address is No. 3 of West Leu 1 in North Star of the Korean region of Beijing city, and the preservation number is CGMCC No.23283. The active polysaccharide of the ganoderma lucidum mutant strain provided by the invention has the effect of obviously better than that of the common strain in the aspect of relieving the drug-induced liver injury, can reduce the damage of the drug to the liver more effectively, enhances the self-repairing capability of the liver, and provides more powerful guarantee for the liver health.

Inventors

  • SU ZHENQUAN
  • WANG QI
  • LI DETIAN
  • LIU YANG

Assignees

  • 安徽斯普瑞生物科技有限公司
  • 吉林农业大学

Dates

Publication Date
20260505
Application Date
20250901

Claims (7)

  1. 1. The application of active polysaccharide of a ganoderma lucidum mutant strain in preparing a medicament for relieving drug-induced liver injury or a health-care product with auxiliary protection effect on chemical liver injury is characterized in that the preparation method of the active polysaccharide of the ganoderma lucidum mutant strain comprises the step of extracting mycelium of a mutant ganoderma lucidum strain JLAU 210928 by using a water extraction and alcohol precipitation method to prepare the active polysaccharide of the ganoderma lucidum mutant strain; The mutagenized ganoderma lucidum strain JLAU 210928 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) for 9 months and 30 days in 2021, the preservation address is North Star Xiyu No.1, 3 in the Korean region of Beijing, and the preservation number is CGMCC No.23283.
  2. 2. The use according to claim 1, wherein the specific steps of the water extraction and alcohol precipitation method comprise: Mixing mycelium of the mutagenized ganoderma lucidum strain JLAU 210928 with water, stirring and extracting, and centrifuging to obtain supernatant; concentrating the supernatant, and adding absolute ethyl alcohol to perform precipitation treatment to obtain a precipitate; And (3) dialyzing the precipitate to obtain the active polysaccharide of the ganoderma lucidum mutant strain.
  3. 3. The use according to claim 2, wherein the temperature of the stirred extraction is 90 ℃ for a period of 3h.
  4. 4. The use according to claim 2, wherein the mycelium of the mutagenized ganoderma lucidum strain JLAU 210928 is mixed with water in a feed liquid mass ratio of 1:30.
  5. 5. The use according to claim 2, wherein the concentration is to concentrate the supernatant to 1/4 of the original volume.
  6. 6. A medicament for alleviating drug-induced liver injury, characterized in that the active ingredient comprises the active polysaccharide of the ganoderma lucidum mutant strain according to any one of claims 1 to 5.
  7. 7. A health product with auxiliary protection effect on chemical liver injury, characterized in that the active ingredient comprises the active polysaccharide of the ganoderma lucidum mutant strain according to any one of claims 1-5.

Description

Active polysaccharide of ganoderma lucidum mutant strain, preparation method and application thereof Technical Field The invention relates to the technical field of biological medicine, in particular to ganoderma lucidum mutant strain active polysaccharide, a preparation method and application thereof. Background Drug-inducedliverinjury (DILI) refers to a disease caused by hepatotoxicity or anaphylaxis of liver to drugs caused by drugs or metabolites thereof, and is one of the most common types of adverse Drug reactions. In recent years, the incidence of liver injury caused by excessive use of acetaminophen has been increasing, and has become a major factor in causing acute liver failure. At present, prevention and treatment of drug-induced liver injury is mainly based on stopping drug and symptomatic treatment, and a specific treatment means is not available. Natural products are paid attention to in the liver protection field because of the characteristics of high safety, wide bioactivity and the like. Ganoderma lucidum (Ganoderma lingzhi) is used as a traditional rare medicinal fungus, and the polysaccharide component contained in the ganoderma lucidum has proved to have various physiological activities and has certain potential in liver protection. However, the polysaccharide of the common ganoderma lucidum strain still has room for improvement in the effect of alleviating the drug-induced liver injury, and is difficult to meet the requirements of clinic and market on high-efficiency liver protection substances, so that the further excavation of functional components with better activity in ganoderma lucidum resources has important significance. Disclosure of Invention The invention aims to provide active polysaccharide of a ganoderma lucidum mutant strain, and a preparation method and application thereof, so as to solve the problems in the prior art. The active polysaccharide of the ganoderma lucidum mutant strain provided by the invention has an effect obviously superior to that of a common strain in the aspect of alleviating drug-induced liver injury, and can reduce the damage of the drug to the liver more efficiently. In order to achieve the above object, the present invention provides the following solutions: The invention provides a preparation method of active polysaccharide of a ganoderma lucidum mutation strain, which comprises the steps of extracting mycelium of a mutation ganoderma lucidum strain JLAU 210928 by using a water extraction and alcohol precipitation method to prepare the active polysaccharide of the ganoderma lucidum mutation strain; The mutagenized ganoderma lucidum strain JLAU 210928 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) for 9 months and 30 days in 2021, the preservation address is North Star Xiyu No. 1, 3 in the Korean region of Beijing, and the preservation number is CGMCC No.23283. Further, the water extraction and alcohol precipitation method specifically comprises the following steps: Mixing mycelium of the mutagenized ganoderma lucidum strain JLAU 210928 with water, stirring and extracting, and centrifuging to obtain supernatant; concentrating the supernatant, and adding absolute ethyl alcohol to perform precipitation treatment to obtain a precipitate; And (3) dialyzing the precipitate to obtain the active polysaccharide of the ganoderma lucidum mutant strain. Further, the temperature of the stirring extraction is 90 ℃ and the time is 3h. Further, the mycelium of the mutagenized ganoderma lucidum strain JLAU 210928 is mixed with water according to a feed liquid mass ratio of 1:30. Further, the concentration is to concentrate the supernatant to 1/4 of the original volume. The invention also provides active polysaccharide of the ganoderma lucidum mutant strain prepared by the preparation method. The invention also provides application of the active polysaccharide of the ganoderma lucidum mutant strain in preparing medicines for relieving drug-induced liver injury. The invention also provides a medicine for relieving the drug-induced liver injury, and the active ingredients comprise the active polysaccharide of the ganoderma lucidum mutant strain. The invention also provides application of the active polysaccharide of the ganoderma lucidum mutant strain in preparing health care products with auxiliary protection effect on chemical liver injury. The invention also provides a health care product with auxiliary protection effect on chemical liver injury, and the active ingredients comprise the active polysaccharide of the ganoderma lucidum mutant strain. The invention discloses the following technical effects: The active polysaccharide of the ganoderma lucidum mutant strain provided by the invention has the effect of obviously better than that of a common strain in the aspect of alleviating the drug-induced liver injury, can reduce the damage of the drug to the liver more efficiently, en