CN-121109057-B - Preparation method of hippocampal oil and application of hippocampal oil in uric acid reducing product

Abstract

The invention discloses a preparation method of sea horse oil, which comprises the following steps of 1) adding sea horse dry powder into dichloromethane-methanol mixed liquid, stirring uniformly, leaching for 24 hours, 2) filtering the leaching liquid, adding water, fully mixing uniformly, standing for 12 hours, collecting a lower organic phase, and 3) rotary evaporating and drying the organic phase. The invention also discloses application of the hippocampus oil in preparing uric acid reducing products. The sea horse oil is rich in omega-3 polyunsaturated fatty acids (EPA and DHA), shows moderate down regulation of xanthine oxidase activity and inhibition of inflammatory factor release, and achieves the comprehensive effects of inhibiting formation and relieving inflammation.

Inventors

• WANG YUMING
• XUE YONG
• XU JIE
• LI ZHAOJIE
• NING YIFEI
• WANG CHENGCHENG
• ZHANG TIANTIAN
• XUE CHANGHU
• CHANG YAOGUANG
• ZOU CAN
• JIANG XIAOMING
• TANG QINGJUAN

Assignees

• 中国海洋大学
• 泉州海洋生物产业研究院

Dates

Publication Date

20260508

Application Date

20251023

Claims (4)

1. 1. Use of hippocampal oil for the preparation of uric acid lowering products, characterized in that the application method of the hippocampal oil is oral administration, and the oral dosage of the hippocampal oil is 400mg/kg body weight/day; The preparation method of the sea horse oil comprises the following steps: 1) The Hippocampus dry powder is prepared by pulverizing freeze-dried fresh Hippocampus with expanded abdomen, adding the Hippocampus dry powder into dichloromethane-methanol mixed solution with volume ratio of 2:1, stirring, and leaching for 24 hr; 2) Filtering the leaching solution, adding water, fully and uniformly mixing, standing for 12 hours, and collecting a lower organic phase; 3) The organic phase was dried by rotary evaporation.
2. 2. The method according to claim 1, wherein the fresh, expanded Hippocampus japonicus is lyophilized at-50deg.C under vacuum of <10kPa for 48 hours.
3. 3. The method according to claim 1 or 2, wherein the dry Hippocampus powder of step 1) is added into a mixed solution of dichloromethane and methanol at a ratio of 1:10.
4. 4. The method of claim 1, wherein the rotary evaporation drying condition in the step 3) is that the vacuum degree is 0.06-0.08 MPa, the rotating speed is 30-60 rpm, the cooling temperature is-10 ℃ and the water bath temperature is 40-50 ℃.

Description

Preparation method of hippocampal oil and application of hippocampal oil in uric acid reducing product Technical Field The invention relates to the technical field of deep processing of marine products, in particular to a preparation method of sea horse oil and application of sea horse oil. Background Hyperuricemia is a metabolic disorder syndrome caused by the increase of serum uric acid concentration, and causes gout, kidney stones and other diseases. The prevalence of hyperuricemia gradually increases with lifestyle changes. At present, medicines for reducing uric acid clinically are classified into xanthine oxidase inhibitors and uric acid excretion medicines. Xanthine oxidase inhibitor has stronger uric acid reducing effect, but adverse reaction and drug interaction limit the use of human groups, uric acid excreting drugs have dependence on renal function, and partial patients have no effect or are intolerant. Furthermore, xanthine oxidase inhibitors and uric acid excretion drugs may cause side effects such as liver and kidney injury, rash, gastrointestinal discomfort, etc. during long-term use. This has prompted attempts to mine new potent uric acid lowering actives from safe natural products, looking for new safe intervention routes to improve hyperuricemia, gout disease. Sea horse related research of marine products is mainly focused on peptides or polysaccharides, and no research report is made on the activity mechanism of sea horse oil phase components. Disclosure of Invention The invention discloses a preparation method of sea horse oil, which comprises the following steps: 1) Adding the sea horse dry powder into a dichloromethane-methanol mixed solution, uniformly stirring, and leaching for 24 hours; 2) Filtering the leaching solution, adding water, fully and uniformly mixing, standing for 12 hours, and collecting a lower organic phase; 3) The organic phase was dried by rotary evaporation. Further, the hippocampal dry powder in step 1) may be prepared by pulverizing freeze-dried fresh, expanded hippocampus. Further, the fresh, expanded hippocampus was freeze-dried at-50 ℃ under vacuum <10kPa for 48 hours. Further, the above-mentioned dichloromethane-methanol mixed solution may be a dichloromethane-methanol mixed solution with a volume ratio of 2:1. Further, the above sea horse dry powder can be added into a dichloromethane-methanol mixed solution according to a feed liquid ratio of 1:10. Further, the condition of rotary evaporation drying can be that the vacuum degree is 0.06-0.08 MPa, the rotating speed is 30-60 rpm, the cooling temperature is-10 ℃, and the water bath temperature is 40-50 ℃. The invention also discloses the sea horse oil prepared by the method. The invention also discloses application of the hippocampus oil in preparing uric acid reducing products. Further, the method of use of the hippocampal oil may be oral. Further, the oral dosage of the hippocampus japonicus oil can be 200-400 mg/kg body weight/day. Specifically, the present invention is as follows. 1. A preparation method of sea horse oil comprises the following steps: 1) Adding the sea horse dry powder into a dichloromethane-methanol mixed solution, uniformly stirring, and leaching for 24 hours; 2) Filtering the leaching solution, adding water, fully and uniformly mixing, standing for 12 hours, and collecting a lower organic phase; 3) The organic phase was dried by rotary evaporation. 2. The method for preparing hippocampal oil according to claim 1, wherein the hippocampal dry powder in step 1) is prepared by pulverizing freeze-dried fresh, expanded hippocampus. 3. The method for preparing hippocampal oil according to claim 2, wherein the condition of freeze-drying the fresh, expanded hippocampus is that the hippocampus is freeze-dried for 48 hours at-50 ℃ under the condition of vacuum degree of <10 kPa. 4. The method for producing hippocampal oil as claimed in any one of the claims 1 to 3, wherein the dichloromethane-methanol mixture in the step 1) is a dichloromethane-methanol mixture with a volume ratio of 2:1. 5. The method for preparing hippocampal oil according to the item 1, wherein the hippocampal dry powder in the step 1) is added into a dichloromethane-methanol mixed solution according to a feed liquid ratio of 1:10. 6. The method for preparing hippocampal oil according to the item 1, wherein the condition of rotary evaporation drying in the step 3) is that the vacuum degree is 0.06-0.08 MPa, the rotating speed is 30-60 rpm, the cooling temperature is-10 ℃, and the water bath temperature is 40-50 ℃. 7. A hippocampal oil prepared by the method for preparing a hippocampal oil according to any one of claims 1 to 6. 8. Use of Hippocampus oil in preparation of uric acid lowering product is provided. 9. The use of the hippocampal oil as recited in claim 8 for preparing uric acid lowering products, wherein the application method of the hippocampal oil is oral administration. 10. The application of the hippocampal oil