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CN-121109152-B - Mucor circinelloides and application thereof

CN121109152BCN 121109152 BCN121109152 BCN 121109152BCN-121109152-B

Abstract

The application discloses Mucor circinelloides and application thereof, wherein the strain number of Mucor circinelloides (Mucor irregularis) is JSAFC and 3408, and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 41982 in the year 05 and 06. According to the application, 1 kind of Mucor circinelloides (Mucor irregularis) is obtained by separating and purifying the soil around the peppers. The strain can inhibit the growth of the yellow-Huai sacculus mildew, promote the growth of the capsicum, regulate and control the growth of plants, and has great significance for the development of biocontrol agents for preventing and controlling the root rot of the capsicum in the future.

Inventors

  • CHEN JIAJIA
  • ZHENG XIANGRONG
  • WU XIANGQIAN
  • QIU XIUWEN
  • FEI FAN
  • LI ZHAOCHENG
  • PENG JINFENG
  • SHAO RAN
  • YU JIAN
  • SUN YAYA
  • FU DANYANG
  • DING YANGFEI
  • HUANG XIAOXIAO
  • LI SONG

Assignees

  • 江苏农林职业技术学院

Dates

Publication Date
20260512
Application Date
20251014

Claims (9)

  1. 1. The Mucor circinelloides (Mucor irregularis) is characterized in that the strain number of the Mucor circinelloides (Mucor irregularis) is JSAFC 3408, and is preserved in China general microbiological culture collection center (CGMCC) with a preservation number of 41982 in the year 05 and the day 06 of 2025.
  2. 2. A biocontrol microbial agent, characterized in that the active ingredient of the biocontrol microbial agent is mucor (Mucor irregularis) with irregularity as described in claim 1.
  3. 3. Use of the mucor circinelloides (Mucor irregularis) of claim 1 and the biocontrol microbial inoculum of claim 2 for inhibiting the growth of saccharum citricum (Globisporangium huanghuaiense).
  4. 4. Use of Mucor circinelloides (Mucor irregularis) according to claim 1, the biocontrol microbial inoculum according to claim 2 for promoting pepper growth.
  5. 5. The use according to claim 4, characterized in that the plant height, leaf and root growth of capsicum is promoted by rooting capsicum with spore suspension of mucor circinelloides (Mucor irregularis) JSAFC 3408.
  6. 6. The method of claim 5, wherein the spore suspension is prepared by (1) taking a strain JSAFC 3408,3408 of biocontrol fungus, picking hyphae with an inoculating needle under aseptic condition, inoculating sterilized PDA culture medium, culturing for 5-7 days, (2) scraping hyphae under aseptic condition, washing the hyphae with aseptic water and filtering with gauze, and (3) adjusting the spore concentration with aseptic water under aseptic condition.
  7. 7. The use according to claim 6, wherein the culture conditions in step (1) are 25 ℃, 65% humidity, dark culture.
  8. 8. The use according to claim 5, characterized in that the concentration of the spore suspension is 10 5 /ml.
  9. 9. The use according to claim 5, wherein the spore suspension is applied at a rate of 5 mL/time/2 days for 30 days.

Description

Mucor circinelloides and application thereof Technical Field The invention belongs to the technical field of microorganisms, and relates to Mucor irregular and application thereof. Background The capsicum is used as an economic crop and a condiment which are widely planted worldwide, and is cultivated in a large area in the north and south of China, and the capsicum has unique flavor and rich nutrition, so that the capsicum becomes an important product in agricultural production. In recent years, as the consumption demand increases, the scale of pepper planting continues to expand, and the intensive and large-scale planting modes are gradually popularized, however, the following disease problems are also increasingly prominent, and root rot has become one of the main obstacles restricting the healthy development of the pepper industry. Huang Huaiqiu cyst mould (Globisporangium huanghuaiense is named Pythium huanghuaiense) is one of pathogens causing pepper root rot, at the initial stage of the disease, the plant root system is browned and rotted, the capability of absorbing moisture and nutrients is greatly reduced, further, symptoms such as yellowing, wilting and sagging of leaves are shown, and when serious, the whole plant withers and dies, so that the yield is reduced and even is dead. Therefore, the development of a novel biocontrol microbial inoculum for preventing and treating the root rot of the capsicum is particularly important. Disclosure of Invention The invention aims to provide an irregular mucor (Mucor irregularis) and application thereof in inhibiting the growth of sacculus flavus and promoting the growth of capsicum. The invention provides an Mucor circinelloides (Mucor irregularis), wherein the strain number of the Mucor circinelloides (Mucor irregularis) is JSAFC 3408, and the Mucor circinelloides is preserved in China general microbiological culture collection center (CGMCC) No. 41982 in 2025, 05 and 06 days. Further, the nucleotide sequence of the ITS gene of Mucor circinelloides is shown as SEQ ID NO. 1. The invention also provides a biocontrol microbial agent, and the active ingredient of the biocontrol microbial agent is the Mucor irregularly (Mucor irregularis). The invention also provides application of the Mucor circinelloides (Mucor irregularis) and the biocontrol microbial inoculum in inhibiting the growth of the sacculus flavus (Globisporangium huanghuaiense). The invention also provides application of the mucor circinelloides (Mucor irregularis) and the biocontrol microbial inoculum in promoting the growth of the capsicum. Further, the application is specifically to promote plant height, leaf and root growth of the capsicum by root irrigation of the capsicum with a spore suspension of mucor circinelloides (Mucor irregularis) JSAFC 3408. Further, the spore suspension is prepared by (1) taking a strain JSAFC and 3408 of biocontrol fungus Mucor, picking hypha by an inoculating needle under aseptic condition, inoculating sterilized PDA culture medium, culturing for 5-7 days, (2) scraping hypha under aseptic condition, flushing the hypha with aseptic water and filtering with gauze, and (3) adjusting spore concentration with aseptic water under aseptic condition. Further, the culture conditions in the step (1) are 25 ℃, 65% humidity and dark culture. Further, the spore suspension had a concentration of 10 5/ml. Further, the spore suspension was applied at 5 mL/time/2 days for 30 days. Compared with the prior art, the method has the remarkable advantage that 1 kind of Mucor (Mucor irregularis) is obtained through separation and purification of the soil around the peppers. The strain can inhibit the growth of the yellow-Huai sacculus mildew, promote the development of capsicum, regulate and control the growth of plants, and has great significance for the development of biocontrol agents for preventing and treating root rot in the future. Drawings The phylogenetic tree of fig. 1:Mucor irregularis JSAFC 3408. FIG. 2 shows the growth of biocontrol bacteria Mucor irregularis JSAFC, 3408 of the invention in opposition to Huang Huaiqiu sporangium. FIG. 3 shows the effect of the biocontrol bacterium Mucor irregularis JSAFC and 3408 of the present invention on the growth of capsicum root systems. Detailed Description The technical scheme of the invention is further described below with reference to the accompanying drawings. As used herein, the term "biocontrol bacteria" refers to beneficial microorganisms, which can control plant diseases, mainly bacteria, fungi, actinomycetes. Example 1:Mucor irregularis JSAFC 3408 isolation and identification 1. Mucor irregularis JSAFC 3408 separation of 3408 The soil with good growth condition of Jurong pepper root system is taken, JSAFC 3408 is obtained by separating the soil from the soil, and the colony is characterized in that the soil is cultivated on a PDA flat-plate culture medium, the colony presents a circular colony, hypha grows luxuriantly and