CN-121109157-B - Phellinus linteus mycelium and preparation method and application thereof

Abstract

The invention belongs to the technical field of biological fermentation, and particularly discloses a Phellinus linteus mycelium and a preparation method and application thereof. The preparation method of the Phellinus linteus mycelium comprises (1) activating strain, inoculating Phellinus linteus mycelium blocks into PDA culture medium for culturing to obtain activated Phellinus linteus, (2) culturing seed, inoculating activated Phellinus linteus into seed culture medium for culturing to obtain Phellinus linteus seed liquid, (3) fermenting and culturing, transferring Sang Huangchong seed liquid into fermentation culture medium for culturing to obtain Phellinus linteus fermentation liquid, (4) filtering and drying, filtering Phellinus linteus fermentation liquid to obtain filter residue, cleaning the filter residue, and oven drying to obtain Phellinus linteus mycelium. The Phellinus linteus mycelium prepared by the invention has high yield and high content of active ingredients, and can be used as a raw material in health products, cosmetics or medicines.

Inventors

• PAN JUWU
• LI LINGFU
• LUO PEIFAN
• CHEN CHENG
• Chen Mingni
• YANG YONGZHI
• LIANG CHENG
• YUAN PING
• WU WENJUN

Assignees

• 南宁汉和生物科技股份有限公司
• 广州市奥雪化工有限公司

Dates

Publication Date

20260512

Application Date

20251117

Claims (1)

1. 1. A preparation method of Phellinus linteus mycelium is characterized by comprising the following steps: (1) Activating strain, namely inoculating Phellinus linteus mycelium blocks into a PDA culture medium for activation culture, and continuously carrying out activation culture for three generations to obtain activated Phellinus linteus; (2) Seed culture, namely inoculating activated Phellinus linteus into a seed culture medium, and culturing for 4-5d at 26-27 ℃ and 200-400rpm to obtain Sang Huangchong seed liquid, wherein the seed culture medium comprises the following components, by mass, 15-20 g/L of glucose, 3-5g/L of yeast powder, 3-5g/L of peptone, 0.1-0.3g/L of magnesium sulfate, 0.4-0.6g/L of monopotassium phosphate and 2-5g/L of mulberry extract; (3) Fermenting and culturing by transferring Sang Huangchong sub-solution into fermentation medium with inoculation amount of 8-10% (v/v), and culturing at 26-27deg.C and 200-500rpm for 5-7d to obtain Phellinus linteus fermentation broth; the fermentation medium comprises the following components of 15-20 g/L of glucose, 5-8g/L of corn meal, 5-8g/L of yeast powder, 1-2g/L of bran, 0.1-0.2g/L of magnesium sulfate, 0.4-0.6g/L of monopotassium phosphate, 3-5g/L of mulberry extract, 2-5g/L of coix seed extract and 1-4g/L of black tea extract, wherein the mulberry extract is a mulberry water extract, the coix seed extract is a coix seed ethanol extract, and the black tea extract is a black tea ethanol extract; (4) Filtering and drying, namely filtering the Phellinus linteus fermentation liquid to obtain filter residues, washing the filter residues with distilled water, centrifuging, drying at 40-60 ℃ to constant weight, crushing and sieving to obtain the Phellinus linteus mycelium.

Description

Phellinus linteus mycelium and preparation method and application thereof Technical Field The invention relates to the technical field of microbial fermentation, in particular to a Phellinus linteus mycelium and a preparation method and application thereof. Background Sang Huangkong fungus genus (Sanghuangporus) is fungus kingdom, basidiomycete phylum, agaricales, hymenochaetales, hymenochaetaceae fungi, is a kind of precious perennial large-scale fungi for both medicine and food, and its fruiting body has long medical history, is known as "forest gold", and is widely used in China, korea, japan and other countries. Related researches show that the main chemical components of the Phellinus linteus contain polysaccharide, flavone, triterpene, phenolic acid, amino acid and the like, and the active substances have good biological activity and no cytotoxicity, and have the effects of resisting cancer, regulating immunity, reducing blood sugar, resisting virus, reducing uric acid, resisting oxidation, resisting inflammation and the like, so that the Phellinus linteus becomes one of the development directions of new drugs. However, phellinus linteus wild resources are scarce, the difficulty of artificial cultivation is high, the yield of cultivation is low at present, and the fruiting body extraction method is polluted, so that the cost is high. The biological fermentation technology has the advantages of wide sources of culture medium raw materials, low cost, short fermentation period, large yield, good controllability and the like, is a more effective and safer method, and can generate a plurality of secondary metabolites with biological activities such as polysaccharides, flavonoids, polyphenols, terpenoids and the like, such as anticancer, anti-inflammatory and antibacterial activities, and abundant extracellular enzymes in the fermentation process, thereby having better research prospect and being widely applied to the culture of medicinal fungus Phellinus. Therefore, the research of the liquid fermentation process with Sang Huanggao effect and good activity effect has important significance for solving the shortage of Phellinus linteus resources, developing active substance products thereof, playing wide application prospect and the like. Disclosure of Invention In view of the foregoing, it would be desirable to provide a Phellinus linteus mycelium and methods for making and using the same. The invention adopts the specific culture medium to carry out primary fermentation treatment on the Phellinus linteus, can effectively improve the yield of Phellinus linteus mycelium and the content of active ingredients, and is beneficial to the subsequent application in medicines. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: A preparation method of Phellinus linteus mycelium comprises the following steps: (1) Activating strain, namely inoculating Phellinus linteus mycelium blocks into a PDA culture medium for activation culture, and continuously carrying out activation culture for three generations to obtain activated Phellinus linteus; (2) Seed culture, inoculating activated Phellinus linteus into seed culture medium, and culturing at 26-27deg.C and 200-400rpm for 4-5d to obtain Sang Huangchong seed solution; (3) Fermenting and culturing by transferring Sang Huangchong sub-solution into fermentation medium with inoculation amount of 8-10% (v/v), and culturing at 26-27deg.C and 200-500rpm for 5-7d to obtain Phellinus linteus fermentation broth; The fermentation medium comprises the following components of 15-20 g/L of glucose, 5-8g/L of corn meal, 5-8g/L of yeast powder, 1-2g/L of bran, 0.1-0.2g/L of magnesium sulfate, 0.4-0.6g/L of monopotassium phosphate, 3-5g/L of mulberry extract, 2-5g/L of coix seed extract and 1-4g/L of black tea extract; (4) Filtering and drying, namely filtering the Phellinus linteus fermentation liquid to obtain filter residues, washing the filter residues with distilled water, centrifuging, drying at 40-60 ℃ to constant weight, crushing and sieving to obtain the Phellinus linteus mycelium. Further, in the step (2), the seed culture medium comprises the following components, by mass, 15-20 g/L of glucose, 3-5g/L of yeast powder, 3-5g/L of peptone, 0.1-0.3g/L of magnesium sulfate, 0.4-0.6g/L of potassium dihydrogen phosphate and 2-5g/L of mulberry extract. Further, in the step (3), the mulberry extract is a mulberry aqueous extract, the coix seed extract is a coix seed ethanol extract, and the black tea extract is a black tea ethanol extract. The invention also provides the Phellinus linteus mycelium prepared by the preparation method. The invention also provides a composition, and the Phellinus linteus mycelium prepared by the preparation method. The invention also provides an application of the Phellinus linteus mycelium or the composition in preparing medicines. The invention has the following beneficial effects: Firstly, the invention s