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CN-121128673-B - Construction method and application of liver injury model of micropterus salmoides

CN121128673BCN 121128673 BCN121128673 BCN 121128673BCN-121128673-B

Abstract

The invention provides a construction method of a liver injury model of a California perch and application thereof, belonging to the field of aquatic medicines, wherein the construction method comprises the following steps of 1) injecting thioacetamide into the abdominal cavity of the California perch with the injection dosage of 100-150mg/kg body weight for 1 time; 2) conventional feeding for 6-15 days. The invention can be used for modeling with short period, low cost, good reproducibility and simple operation, and solves the problem of high death rate of animals when the acute liver injury model is manufactured in the prior art.

Inventors

  • QI QIONG
  • YAN YONG
  • LV HUIYUAN
  • LI MING
  • YU JINGCHENG
  • QIN ZHE
  • WANG XIUMIN

Assignees

  • 北京生泰尔科技股份有限公司
  • 生泰尔(内蒙古)科技有限公司

Dates

Publication Date
20260505
Application Date
20250904

Claims (9)

  1. 1. The construction method of the liver and pancreas injury model of the micropterus salmoides is characterized in that the liver and pancreas injury model is an acute liver injury model, and comprises the following steps: 1) The method comprises the steps of injecting thioacetamide into the abdominal cavity of the micropterus salmoides at a dosage of 100-150mg/kg body weight for 1 time, injecting thioacetamide solution at an injection amount of 0.05-0.1mL, and selecting young micropterus salmoides with a body weight of 15-100 g/strip; 2) Conventional feeding for 6-8 days to obtain the feed; setting a rating score related to liver injury symptoms, and establishing a grading rating method, wherein the grading rating method comprises liver rating, body surface rating and abdomen rating, and the rating method comprises the following steps: the sum of the liver score, the body surface score and the abdomen score is used as a liver injury comprehensive score; and when one of the liver injury symptoms in the scoring table appears in the experimental animal, and the sampling injury proportion is more than or equal to 80%, the model is considered to be successfully constructed.
  2. 2. The method of claim 1, wherein in step 1), thioacetamide is injected in the form of a solution.
  3. 3. The method of claim 1, wherein in step 1), thioacetamide is dissolved in sterilized ultrapure water.
  4. 4. The method of claim 1, wherein, in step 1), the young fish having a weight of 25-30 g/strip is selected from the group consisting of micropterus salmoides.
  5. 5. The construction method according to claim 1, wherein in step 2), the constructed model is used for screening products for preventing and/or treating liver diseases of fish, which is a california weever.
  6. 6. The construction method according to claim 1, wherein in step 2), the feed is a conventional feed, 2 meals a day are fed, the daily feeding amount is 1-5% of the body weight, the satiety feeding is carried out, and the feeding is continued for 1-2 weeks.
  7. 7. The construction method according to any one of claims 1 to 6, wherein the feeding feed comprises, by weight, 8-12 parts of soybean meal, 0.5-1.5 parts of wheat gluten, 5-10 parts of fermented soybean meal, 15-25 parts of domestic defatted fish meal, 25-35 parts of super imported steam fish meal, 10-15 parts of imported chicken meal, 1-3 parts of plasma protein powder, 1-3 parts of seaweed meal, 2-6 parts of tapioca starch, 5-10 parts of high gluten flour, 4-8 parts of fish oil, 1-3 parts of aquatic multi-vitamins and 2-4 parts of aquatic multi-minerals.
  8. 8. The grading evaluation method of the liver and pancreas injury model of the micropterus salmoides is characterized by comprising the steps of: the sum of the liver score, the body surface score and the abdomen score is used as a liver injury composite score.
  9. 9. Use of a model constructed according to the method of any one of claims 1 to 7 for screening products for the prevention and/or treatment of liver disease in fish; wherein the fish comprises one or more of Carnis Perch, grass carp, channel catfish, yellow catfish, carp, large yellow croaker, tilapia, and snakehead.

Description

Construction method and application of liver injury model of micropterus salmoides Technical Field The invention relates to the technical field of aquatic medicament development, in particular to a construction method and application of a liver injury model of a micropterus salmoides, which are used for screening products (such as aquatic medicaments) for preventing and/or treating fish liver diseases. Background Along with the improvement of the fish culture density, the deterioration of the culture water environment, the misuse of medicines, unbalanced nutrition of the feed, frequent diseases of the cultured fish caused by harmful substances in the feed and other factors, and huge losses are caused for fishery production. Various factors can cause fatty liver diseases of cultured fishes, such as feed factors (nutrient imbalance, oxidized grease and the like), mycotoxins (aflatoxins, vomitoxins and the like), harmful drugs (sulfonamides, furans and the like) and cultural water environment deterioration (exceeding ammonia nitrogen and nitrous acid, hypoxia, heavy metal poisoning and the like) and the like. If liver diseases occur in cultured fishes, mild people influence the growth speed, production potential and feed conversion efficiency of the fishes, and meanwhile influence normal immune function and physiological metabolism of the fishes, and serious people suffer from liver and gall comprehensive diseases, such as steatohepatitis, fatty liver, liver and gall syndrome, liver and kidney syndrome and the like, when various syndromes occur, the diseases are predicted to indicate the multiple organ failure syndrome of the fishes, and finally a large amount of death of the fishes is caused. The current problem of liver and gall of aquatic animals is widely spread, so that the fish such as grass carp, pelteobagrus fulvidraco, large yellow croaker, tilapia, mackerel and snakehead are affected. Both juvenile and adult fish may be affected. In the aquaculture industry, some farmers seek to maximize profit, improper cultivation, excessive use of drugs and additives, and serious injury to the liver of aquatic animals. The current liver injury model construction has the problems that the influence of selection of medicament types and doses on the model construction is relatively large, for example, liver injury is not obvious due to the fact that the doses are too low, experimental requirements are difficult to meet, acute liver failure is caused due to the fact that the doses are too high, a large number of experimental animals die, the sensitivity of different types of animals to medicaments is different, a certain time is needed for accumulation when the medicaments are used for inducing the liver injury model, and continuous administration is usually needed for a plurality of days to a plurality of weeks. However, too long induction time may lead to reduced immunity of the fish body and increased risk of secondary infection, and too short induction time may not form a stable liver injury model. In addition, the aquatic animals have lower metabolic enzyme activity and weaker detoxification capability, and further increase the difficulty of the liver injury model of the micropterus salmoides. Under experimental conditions, to investigate and study the occurrence mechanism and process of liver lesions of cultured fish, a liver injury model of the micropterus salmoides needs to be established. However, no method for copying batch, controllable and consistent pathological changes of liver pathological changes of the micropterus salmoides in a limited time exists at present. Disclosure of Invention Object of the Invention The invention aims to provide a construction method and application of a liver injury model of a micropterus salmoides. The invention can be used for modeling with short period, low cost, good reproducibility and simple operation, and solves the problem of high death rate of animals when the acute liver injury model is manufactured in the prior art. Batch, controlled, consistently diseased fish liver samples can be replicated in a limited period of time (e.g., day 3). Through a fish liver injury experiment, a fish liver and pancreas injury experimental model and a corresponding evaluation index system thereof are established, and an experimental platform is provided for discussing and researching the occurrence, development mechanism and process of fish liver lesions and screening prevention and treatment drugs or feed additives. Solution scheme In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: In a first aspect, the invention provides a construction method of a liver and pancreas injury model of a micropterus salmoides, which comprises the following steps: 1) The abdominal cavity of the weever is injected with thioacetamide (TAA for short) with the injection dosage of 100-150mg/kg body weight for 1 time; 2) And (5) conventionally raising