CN-121137000-B - Auxin response SAUR gene family protein gene and application thereof

Abstract

The invention discloses an auxin response SAUR gene family protein gene and application thereof, belonging to the technical field of genetic engineering. The invention discovers for the first time that the expression pattern of the auxin response SAUR gene family protein gene HvSAUR shows a significant positive correlation with accumulation of valine, leucine and isoleucine. According to the invention, hvSAUR over-expression vectors are constructed and transformed into tobacco, and the detection shows that the contents of valine, leucine and isoleucine in transgenic plant leaves are obviously improved compared with the wild type. The coded HvSAUR-gene-responsive SAUR gene family protein plays a key catalytic role in the biosynthesis pathway of branched-chain amino acids of plants, provides an important target gene for improving crop quality, and has good application prospects as well as recombinant vectors, recombinant bacteria and transgenic plants thereof.

Inventors

• Dunzhu Gabu
• Ba Sangyuzhen
• WANG MU
• XU CONGPING
• ZENG XINGQUAN

Assignees

• 西藏自治区农牧科学院农业研究所
• 武汉轻工大学

Dates

Publication Date

20260508

Application Date

20250912

Claims (7)

1. 1. The application of a gene fragment with a nucleotide sequence shown as SEQ ID NO.1, a recombinant vector containing the gene fragment, recombinant bacteria containing the recombinant vector or protein obtained by expressing the gene fragment with the nucleotide sequence shown as SEQ ID NO.1 in preparing transgenic plants with high expression branched-chain amino acid is disclosed, wherein the plants are tobacco.
2. 2. The use according to claim 1, wherein the recombinant vector is recombinant pEAQ or recombinant pCXSN.
3. 3. The use according to claim 1, wherein the recombinant bacterium is a recombinant agrobacterium.
4. 4. The use according to claim 3, characterized in that the agrobacterium is EHA105.
5. 5. A construction method of a transgenic plant with high-expression branched-chain amino acid is characterized in that a recombinant vector containing a gene fragment with a nucleotide sequence shown as SEQ ID NO.1 is transferred into plant leaves, the plant leaves are cultured in a differentiation culture medium until adventitious buds grow, and the plant leaves are transferred into a growth culture medium for continuous culture, so that the transgenic plant with high-expression branched-chain amino acid is obtained, and the plant is tobacco.
6. 6. The method of claim 5, wherein the recombinant vector is recombinant pEAQ or recombinant pCXSN.
7. 7. The method of claim 5 or 6, wherein the method for transferring into plant leaves is Agrobacterium, particle gun, electrotransfer, PEG-mediated, liposome or calcium phosphate-DNA co-precipitation.

Description

Auxin response SAUR gene family protein gene and application thereof Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to an auxin response SAUR gene family protein gene and application thereof. Background Highland barley is an important food crop widely planted in a plurality of regions worldwide, has higher nutritive value and is also paid attention to due to the adaptability of the highland barley in severe environments. Highland barley kernels contain rich essential amino acids (such as valine (Val), leucine (Leu) and isoleucine (Ile)), which are important nutritional ingredients that cannot be synthesized by the human body and must be taken from food, and have important significance for human health. However, despite the abundant amino acid content in highland barley, the current genetic excavation work for the synthesis and regulation of the amino acid is still in a starting stage, which limits the deep understanding of highland barley amino acid metabolism network to a certain extent and further improves the potential of the amino acid content by genetic engineering means. In the process of plant growth and metabolism regulation, auxin plays an extremely important role as a key plant hormone. The method can influence the morphogenesis and the growth and development of plants by regulating and controlling various physiological processes such as cell elongation, division, differentiation and the like. In recent years, SAUR (Small Auxin Up RNA) gene family, which is an important member of auxin response family, is becoming a hotspot in the field of botanic research. SAUR gene family members are widely present in a variety of plants, are capable of rapidly responding to auxin signals, and are involved in the regulation of plant growth and development by a variety of mechanisms. Studies in arabidopsis and other model plants show that SAUR gene family members regulate cell elongation and growth by regulating cell wall relaxin activity, affecting intracellular ion balance, participating in cell signal transduction and other various pathways. However, there are relatively few studies on the function of the SAUR gene family in highland barley and its potential role in the regulation of amino acid metabolism, and there is no systematic and intensive discussion. The functions of SAUR gene families in highland barley and the relation between the functions and amino acid metabolism are deeply excavated and researched, and the highland barley has important theoretical significance and application value for comprehensively knowing the growth and development mechanism of highland barley and cultivating highland barley varieties with higher amino acid content, and is hopeful to provide new ideas and targets for genetic improvement of highland barley. Disclosure of Invention In order to solve the above problems in the prior art, the present invention aims to provide an auxin responsive SAUR gene family protein gene and its use. In order to achieve the above purpose, the present invention adopts the following technical scheme: The invention provides a gene fragment, and the nucleotide sequence of the gene fragment is shown as SEQ ID NO. 1. The invention also provides a recombinant vector which comprises a gene fragment with a nucleotide sequence shown as SEQ ID NO. 1. Further, the recombinant vector is recombinant pEAQ or recombinant pCXSN. The invention also provides a recombinant bacterium, which comprises the recombinant vector. Further, the recombinant bacterium is recombinant agrobacterium. Further, the agrobacterium is EHA105. The invention also provides a protein which is obtained by expressing a gene fragment with a nucleotide sequence shown as SEQ ID NO. 1. The invention also provides application of the gene fragment, the recombinant vector, the recombinant bacterium and the protein in preparing transgenic plants with high expression branched-chain amino acid. Further, the plant is highland barley or tobacco. The invention also provides a construction method of the transgenic plant, which comprises the steps of transferring the recombinant vector into plant leaves, culturing the plant leaves in a differentiation medium until adventitious buds grow, and transferring the plant leaves into a growth medium for continuous culture. Further, the plant is highland barley or tobacco. Further, the method for transferring into plant leaves is an agrobacterium method, a gene gun method, an electrotransfer method, a PEG-mediated method, a liposome method or a calcium phosphate-DNA coprecipitation method. The invention has the following beneficial effects: The invention discovers that the expression mode of the auxin response SAUR gene family protein gene HvSAUR and the accumulation of essential amino acids valine (Val), leucine (Leu) and isoleucine (Ile) show obvious positive correlation (Pearson correlation coefficient r >0.8, p < 0.01) for the first time through