CN-121160713-B - Application of gene MtUMAMIT in regulation and control of growth and quality of forage grass

CN121160713BCN 121160713 BCN121160713 BCN 121160713BCN-121160713-B

Abstract

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT in regulating and controlling growth and development of forage grass and quality. The biological function of the gene MtUMAMIT is first identified, the MtUMAMIT gene is screened and locked through multiple groups of chemical analysis, the phenotype of the homozygous mutant NF8465/NF12630 is analyzed, the fact that the downregulation of the gene MtUMAMIT leads to the growth retardation of the medicago truncatula, the lignin content is reduced, the positive regulation of the growth and development of the medicago truncatula, the branch number, the leaf size and the like by the gene MtUMAMIT is clear, the lignin synthesis is negatively regulated, and a novel gene resource is provided for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

Inventors

BAI LIU
SUN ZHANMIN
ZHAO LINA
WU YANMIN
SHI FENGLING
LIANG ZHE
ZHANG YUTONG
TANG YIXIONG
ZHANG ZHIQIANG

Assignees

中国农业科学院生物技术研究所

Dates

Publication Date: 20260512
Application Date: 20250428

Claims (5)

1. The application of reducing the expression quantity of the gene MtUMAMIT in forage grass in regulating the growth and development of the forage grass is characterized in that the nucleotide sequence of a coding region of the gene MtUMAMIT is shown as SEQ ID NO. 1, the sequence of a coding protein of the gene MtUMAMIT is shown as SEQ ID NO. 2, the regulation and the development of the forage grass are realized in such a way that the root length, the lateral root number, the leaf area, the plant height, the lateral branch number or the branch number of the forage grass are reduced, and the forage grass is medicago terrestris.
2. The application of reducing the expression quantity of the gene MtUMAMIT in forage grass in regulating and controlling the quality of the forage grass is characterized in that the nucleotide sequence of a coding region of the gene MtUMAMIT is shown as SEQ ID NO. 1, the sequence of a coding protein of the gene MtUMAMIT is shown as SEQ ID NO. 2, the quality of the forage grass is reduced, and the forage grass is medicago terrestris.
3. A method for regulating the growth and development of forage grass is characterized by comprising the step of reducing the expression of a gene MtUMAMIT in the forage grass, wherein the nucleotide sequence of the gene MtUMAMIT is shown as SEQ ID NO. 1, the regulation and development of the forage grass is realized by reducing the root length, the lateral root number, the leaf area, the plant height, the lateral branch number or the branch number of the forage grass, and the forage grass is medicago sativa.
4. A method for reducing lignin content of forage grass is characterized by comprising the step of reducing expression of a gene MtUMAMIT in forage grass, wherein a nucleotide sequence of the gene MtUMAMIT is shown as SEQ ID NO. 1, and the forage grass is medicago terrestris.
5. The application of reducing the expression level of the gene MtUMAMIT in forage grass in breeding forage grass varieties with low lignin content is characterized in that the nucleotide sequence of the coding region of the gene MtUMAMIT8 is shown as SEQ ID NO. 1, and the forage grass is medicago terrestris.

Description

Application of gene MtUMAMIT in regulation and control of growth and quality of forage grass Technical Field The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT in regulating and controlling growth and development of forage grass and quality. Background The transport protein (Transport Proteins) is used as an important functional protein inlaid on a cell membrane or a cell organelle membrane, can accurately mediate substances such as ions, small molecular organic matters (such as saccharides and amino acids), signal molecules and the like to realize transmembrane transport, and plays an irreplaceable core role in basic vital activities such as organism nutrient substance intake, metabolic waste discharge, cell signal transduction, plant organ morphogenesis and the like. However, due to the huge transporter family, high complexity of gene composition and significant functional redundancy among members, there is still a large gap in analyzing the specific physiological functions of most transporters, and related research is urgently needed to be advanced. UMAMIT the transporter is an important member of the protein transport superfamily, comprising 44 and 53 family members in Arabidopsis and rice, respectively, model plants. Although the biological function of the part UMAMIT gene in Arabidopsis has been revealed initially, the whole study is still in the initiation phase and a large number of gene functions have not been elucidated yet. Particularly in alfalfa of leguminous plants, the UMAMIT gene family member MtUMAMIT has not been reported in the literature, and the function report is not seen, and the function of the member MtUMAMIT cannot be predicted by the person of ordinary skill in the art, so that the excavation and utilization of the gene resource have important research significance and application value. Tribulus herba Medicaginis (Medicago truncatula) is used as annual herb of Leguminosae genus, and has become ideal mode plant for Leguminosae plant genetics research by virtue of its abundant natural population genetic diversity, wide mutant and ecological resource library, short growth cycle, compact genome structure and high-efficiency stable genetic transformation system. Meanwhile, the Medicago sativa and the Medicago sativa have high affinity similarity, so that the Medicago sativa has great application potential in the field of genetic improvement of forage crops, however, in forage breeding and animal husbandry production, the high lignin content in forage is a long-standing technical problem, which affects the absorption of animals to forage nutrition, the forage with low lignin content is urgently needed to be developed, the traditional breeding means are difficult to accurately regulate lignin, the breeding period is long, and new technical schemes are urgently needed to be searched for to solve the problem of high lignin content in forage. Disclosure of Invention Based on the technical problems, candidate genes are screened from the genome of the medicago truncatula through multiple groups of chemical analysis, the gene MtUMAMIT is obtained through a gene recombination technology, the gene homozygous mutant is further obtained through screening, the function of the gene is deeply researched, the gene is found to have a regulation and control effect on the growth and development of the medicago truncatula and the lignin content, and a new gene resource is provided for improving the yield and quality of forage grass such as medicago truncatula. On the one hand, the invention provides application of a gene MtUMAMIT8 in regulating the growth and development of forage grass, the nucleotide sequence of a coding region of the gene MtUMAMIT is shown as SEQ ID NO. 1, and the sequence of a coding protein of the gene MtUMAMIT is shown as SEQ ID NO. 2. Further, in the application, the forage grass is medicago terrestris. Further, in the application, the expression quantity of the gene MtUMAMIT in the medicago truncatula is reduced, the growth of forage grass is blocked, and the gene MtUMAMIT has positive regulation and control effects in the growth and development of the medicago truncatula. In a second aspect, the invention provides application of a gene MtUMAMIT8 in regulating the quality of forage grass, wherein the nucleotide sequence of a coding region of the gene MtUMAMIT is shown as SEQ ID NO. 1, the nucleotide sequence of a coding protein of the gene MtUMAMIT is shown as SEQ ID NO.2, and the regulation of the quality of forage grass is to reduce the lignin content in the forage grass. Further, in the application, the forage grass is medicago terrestris. Further, in the application, the expression level of the gene MtUMAMIT in the medicago truncatula is reduced, the quality of the medicago truncatula is improved, and the improvement of the quality of the medicago truncatula is that the content of lignin in the medicago truncatula is