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CN-121176322-B - High-glucan rice planting method based on microbial enzymes

CN121176322BCN 121176322 BCN121176322 BCN 121176322BCN-121176322-B

Abstract

The invention relates to the technical field of agricultural biology, and discloses a high-glucan rice planting method based on microbial enzymes, which comprises the following steps: and (3) carrying out ventilation fermentation on raw materials such as barley flour, mushroom mycelium powder and the like to prepare composite enzyme liquid containing beta-amylase and glucose polymerase with specific activity ratio, treating the whole process from seeds to maturity of rice by using the enzyme stock solution prepared in the step S1, and carrying out biological regulation and control by using the composite enzyme liquid in the whole growth period of the rice, wherein in the grouting period, the starting time of spike spraying is determined by monitoring the stable descending trend of the relative chlorophyll content of the sword-leaf of the rice from a peak value. According to the invention, through the accurate coupling of the enzymatic reaction system and the plant endogenous substance transfer rhythm, the glucan content in the finally-harvested rice is obviously improved under the condition of not applying chemical synthesis input products, and the taste quality indexes such as chalky grain rate and the like are improved, and meanwhile, the crop yield is maintained.

Inventors

  • Sheng Taofeng

Assignees

  • 湖北楚洪农业科技有限公司

Dates

Publication Date
20260505
Application Date
20250901

Claims (9)

  1. 1. The high-glucan rice planting method based on the microbial ferment is characterized by comprising the following steps of: s1, preparing a composite enzyme solution: Evenly mixing 48-52% of barley flour, 28-32% of mushroom mycelium powder, 9-11% of brown sugar and 9-11% of softened water according to mass percent, then carrying out aeration fermentation for 95-105 hours at the temperature of 28-32 ℃ until the pH value of fermentation liquor is stabilized within the range of 3.8-4.2, and finally filtering to obtain ferment stock solution; s2, seed soaking treatment: diluting the ferment stock solution and water according to the volume ratio of 1:5, and soaking rice seeds for 7-9 hours; s3, biological regulation and control in the whole growth period: treating in a plurality of rice growing stages by using the ferment stock solution prepared in the step S1, wherein the growing stages at least comprise a three leaf one heart stage, a sealing stage, a breaking stage and a grouting stage; Wherein the ratio of beta-amylase activity in the barley flour to glucose polymerase activity in the mushroom mycelium powder is 1.2:1 to 1.5:1.
  2. 2. The method according to claim 1, wherein the grouting period in the step S3 is performed by spike spraying, and the spike spraying treatment using the ferment stock solution prepared in the step S1 is mainly triggered and performed by the following methods: and (3) regularly monitoring the SPAD value of the chlorophyll relative content of the rice sword leaf, and starting spraying on the spike of the rice within 48 hours after the SPAD value is monitored to be transferred from the peak value platform stage to the stable descending trend.
  3. 3. The method according to claim 1, wherein the three-leaf one-core period, the sealing period and the breaking period are all treated by foliar spraying, specifically, the ferment stock solution and water are diluted according to the volume ratio of 1:3, and the spraying is carried out with the dosage of 4.5-5.5L per mu.
  4. 4. The method of claim 3, wherein the foliar spraying is performed by an unmanned aerial vehicle, and the median value of the droplet size is less than or equal to 150 μm.
  5. 5. The method according to claim 2, wherein the ear spraying in the grouting period is characterized in that the ferment stock solution and water are diluted according to the volume ratio of 1:3, the ferment stock solution is sprayed to the rice ear with the dosage of 2.8-3.2L per mu, and the ear spraying is carried out once every 4-6 days after the ear spraying, and the total spraying is carried out for 3 times.
  6. 6. The method according to claim 1, wherein the biological control in the whole growth period further comprises a supplementary spraying step, namely, after any foliar spraying, if the effective rainfall exceeds 5mm in 12 hours, carrying out supplementary spraying once according to the original dosage in 24 hours after weather is changed.
  7. 7. The method according to claim 1, wherein no chemically synthesized fertilizers, pesticides, herbicides and plant growth regulators are applied during the whole rice growth cycle.
  8. 8. The method according to claim 1, further comprising selecting a rice variety having a consistency of not less than 85mm prior to said seed soaking treatment.
  9. 9. The process according to claim 2, characterized in that the aerated fermentation in step S1 is carried out with stirring at a rate of 45-55 r/min.

Description

High-glucan rice planting method based on microbial enzymes Technical Field The invention relates to the technical field of agricultural biology, in particular to a high-glucan rice planting method based on microbial enzymes. Background Rice is a global main grain crop, nutrition enhancement and functional improvement of the rice are important directions for guaranteeing human health, glucan is used as a functional polysaccharide, beta-1, 3 and beta-1, 4 glycosidic bonds contained in the molecular structure of the glucan endow the glucan with specific physiological activities of regulating intestinal flora and the like, however, the content of the glucan in grains of common rice varieties is extremely low and is usually less than 1%, and the requirement of functional food development cannot be met. In order to improve the content of glucan in rice, the prior art explores different paths, the traditional hybridization breeding method has long period, and because of the genetic complexity of target characters, efficient directional breeding of specific chemical components is difficult, and the method for modifying starch synthesis related enzymes to change the types of products by using a gene editing technology faces disputes of biological safety and strict market regulation limits. On the cultivation regulation level, although the application of a chemical inducer can influence the metabolism of seeds, the application is often accompanied with the risk of exceeding the standard of pesticide residues, researches show that the rice varieties with high partial gum consistencies provide favorable substrate basis for the conversion of starch to glucan because of high amylopectin content, even if the conversion potential exists, the current technical field still lacks a high-efficiency and safe biotechnology means capable of utilizing the potential and realizing the bioconversion process, the existing general microbial fertilizer or biostimulant has the action mechanism mainly for improving the integral growth situation or nutrient absorption of plants and not pertinently catalyzing the directional conversion of carbohydrate metabolism paths in the seeds to glucan, so that the regulation specificity and efficiency are insufficient, and the development of a planting method capable of avoiding the problems of biosafety and chemical residues and accurately and efficiently realizing the directional accumulation of glucan in rice seeds is a technical problem waiting to be solved in the field. Disclosure of Invention The technical problem to be solved by the invention is that the existing rice planting technology lacks a high-efficiency and safe biological regulation means which is accurately matched with the physiological state of rice in terms of improving specific functional components in seeds, the intervention measures in the prior art are often based on fixed lunar calendar, the accuracy is insufficient, and part of the methods depend on chemical inducers and have food safety risks. In order to solve the technical problems, the invention provides a high-glucan rice planting method based on microbial enzymes. The technical scheme provided by the invention is as follows: a high-glucan rice planting method based on microbial enzymes comprises the following steps: s1, preparing a composite enzyme solution: The preparation method comprises the following steps of preparing an enzyme stock solution for subsequent treatment: mixing raw materials, namely weighing 48-52% of barley flour, 28-32% of mushroom mycelium powder, 9-11% of brown sugar and 9-11% of softened water according to mass percentage, and placing the barley flour, the mushroom mycelium powder and the softened water in a container for uniform mixing, wherein the barley flour provides a material basis for the production of beta-amylase, and the mushroom mycelium powder provides a material basis for the production of glucose polymerase. And (3) fermenting, namely placing the uniformly mixed materials at a temperature of 28-32 ℃ for ventilation fermentation, wherein the fermentation process lasts for 95-105 hours, and the fermentation process is performed under the condition of stirring at a speed of 45-55r/min for ensuring the uniformity of a fermentation system, and the judgment standard of the fermentation end point is that the pH value of the fermentation liquid is stable within a range of 3.8-4.2. And filtering, namely filtering the fermentation liquor after fermentation is finished, and removing solid residues, wherein the obtained filtrate is the ferment stock solution. In a specific technical scheme, the quality of the prepared ferment stock solution is controlled, the ratio of the activity of beta-amylase to the activity of glucose polymerase is controlled within the range of 1.2:1 to 1.5:1, the enzyme activity ratio forms a catalytic basis for efficiently converting starch into glucan, wherein the beta-amylase is responsible for hydrolyzing starch end