CN-121181383-B - Trichoderma fungicide for promoting growth of allium plants and application of trichoderma fungicide

Abstract

The invention provides a trichoderma microbial inoculum for promoting the growth of allium plants and application thereof, wherein the trichoderma microbial inoculum is trichoderma harzianum (Trichoderma harzianum) strain MCBC3, the trichoderma microbial inoculum is applied with diammonium phosphate, the application amount of the diammonium phosphate is 15 kg/mu, the application amount of the trichoderma microbial inoculum is 1L/mu, the existing trichoderma resource development is concentrated in a normal soil environment, the trichoderma harzianum MCBC3 is screened out from saline-alkali soil for the first time, and the salt tolerance and alkali tolerance of the trichoderma harzianum MCBC3 are directly against the double stress of high salt and high alkali of the saline-alkali soil, so that the technical bottlenecks of poor adaptability and low survival rate of the traditional strain in extreme environments are solved.

Inventors

• LIU CHANGTAO
• ZHANG FENGGE
• YANG KEMING
• LI LE
• JIA ZHENYU
• WANG ZHAOMING
• YU LIXIA
• ZHANG ZHIXIA
• YAN YIRU
• YAN RUIZHI
• GUO JIALU
• LIANG JIAYI

Assignees

• 蒙草生态环境(集团)股份有限公司
• 蒙草生态环境(集团)股份有限公司海南分公司

Dates

Publication Date

20260512

Application Date

20251121

Claims (5)

1. 1. The application of trichoderma microbial inoculum for promoting the growth of allium plants, which is characterized in that the trichoderma microbial inoculum is used for promoting the growth of allium plants in saline-alkali soil areas, wherein the allium plants comprise allium tuberosum and allium schizandrum; The application method comprises the steps of preparing the trichoderma reesei microbial inoculum and diammonium phosphate, wherein the application amount of the diammonium phosphate is 15 kg/mu, and the application amount of the trichoderma reesei microbial inoculum is 1L/mu; The trichoderma microbial inoculum is trichoderma harzianum (Trichoderma harzianum) strain MCBC3, the preservation number of which is CGMCCNo.41745, ITS, and the sequence of which is shown in SEQ ID NO. 1; the strain MCBC3 has salt resistance and alkali resistance, and has growth capacity under the conditions of 800 mmol NaCl and 0.5 percent sodium bicarbonate.
2. 2. The use according to claim 1, wherein the strain MCBC3 is grown at a temperature of 28 ℃ and a pH=5.0-6.0, and wherein the carbon source is glucose or sucrose, and is cultured on PDA medium.
3. 3. The use according to claim 1, characterized in that the application method comprises spraying Shi Mu mould agent on the roots of allium plants while applying diammonium phosphate.
4. 4. The use according to claim 1, wherein the trichoderma reesei bacterial agent is sprayed 1 time per week for 3 consecutive times.
5. 5. The use according to claim 1 for improving the quality of saline-alkali soil.

Description

Trichoderma fungicide for promoting growth of allium plants and application of trichoderma fungicide Technical Field The invention relates to the technical field of microorganisms, in particular to a trichoderma fungicide for promoting the growth of allium crops in saline-alkali soil areas and application thereof. Background Allium (Allium l.) is a world important vegetable, medicinal and ornamental plant resource, of which Allium tuberosum and Allium schneiderianum are two typical Allium plants, mainly distributed in northern grassland areas in our country. The Allium tuberosum (Allium senescens l.) is a perennial herb of Allium genus of the family liliaceae, the Allium fistulosum (Allium schoenoprasum) is a bulb plant of Allium genus (Allium) of the family liliaceae (LILIACE AE), the two crops have feeding, edible and medicinal values at the same time, and the different growth stages of the Allium tuberosum contains higher protein, so that the Allium tuberosum is the first choice pasture for fattening livestock. Along with the increasing demands of people on the leek and the chives, the cultivation area and the growth range of the leeks need to be continuously enlarged, but the available planting areas are mostly arid and semi-arid early areas, the water resource conditions are very limited, and the soil salinization degree is serious, so that an effective way for improving the soil quality and promoting the growth of crops needs to be found. The application of the microbial preparation is an effective measure for improving the saline-alkali soil, the trichoderma is fungus of the ascomycota, the ascomycota has the characteristics of strong decay, rapid growth and propagation and the like, can rapidly utilize nutrients in soil to realize amplification, forcefully colonize and survive in plant rhizosphere for a long time, and is a beneficial soil microorganism. However, at present, the excavation application of trichoderma resources in China is mainly focused on a normal growth environment, but trichoderma strain resources in a stress environment are not yet effectively developed. Therefore, the dominant trichoderma strains in the saline-alkali stress environment are excavated, and the storage and application are carried out, so that the growth-promoting bacterial fertilizer product aiming at the growth of the allium crops in the saline-alkali soil can be developed, and the application prospect is good. Disclosure of Invention The invention provides a trichoderma fungicide for promoting the growth of allium plants and application thereof, which specifically comprises the following steps: A trichoderma fungicide for promoting the growth of allium plants is trichoderma harzianum (Trichoderma harzianum) strain MCBC3, the preservation number of which is CGMCCNo.41745, ITS, and the sequence of which is shown in SEQ ID NO. 1. The growth condition of the strain MCBC3 is that the culture temperature is 28 ℃, the pH=5.0-6.0, the carbon source is glucose or sucrose, and the strain MCBC3 is cultured on a PDA culture medium. Moreover, the strain MCBC3 has salt tolerance and can grow under the condition of 800mmol NaCl. Moreover, the strain MCBC3 has alkali resistance and can grow under the condition of 0.5% sodium bicarbonate. On the other hand, the invention provides application of trichoderma microbial inoculum for promoting the growth of allium plants in saline-alkali soil areas or improving the quality of the saline-alkali soil. Moreover, the Allium plants include Allium schoenoprasum and Allium schoenoprasum. Furthermore, the application method comprises spraying Shi Mu mould agent on the root of the allium crop, and simultaneously applying diammonium phosphate. Furthermore, the spraying amount was 1L/mu, and the spraying was performed 1 time per week and 3 times continuously. And the trichoderma microbial inoculum and the diammonium phosphate are applied, the application amount of the diammonium phosphate is 15 kg/mu, and the application amount of the trichoderma microbial inoculum is 1L/mu. Compared with the prior art, the invention has the following beneficial effects: 1. The invention directly solves the problems of poor soil quality and low nutrient availability of saline-alkali soil by the application of MCBC3 microbial inoculum and diammonium phosphate, and simultaneously promotes the growth of crops and matches the agricultural production requirements of the northern grassland area. 2. The existing trichoderma resource development is concentrated in a normal soil environment, and the trichoderma harzianum MCBC3 is screened out from the saline-alkali soil for the first time, and the salt tolerance and the alkali tolerance of the trichoderma harzianum MCBC3 are directly aimed at the dual stress of high salt and high alkali in the saline-alkali soil, so that the technical bottlenecks of poor adaptability and low survival rate of the traditional strain in an extreme environment are solved. 3. The strain MCBC3