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CN-121204131-B - WRKY transcription factor gene StWRKY and application thereof

CN121204131BCN 121204131 BCN121204131 BCN 121204131BCN-121204131-B

Abstract

The invention discloses a WRKY transcription factor gene StWRKY and application thereof, belonging to the technical field of biology. The nucleotide sequence of STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. The StWRKY gene provided by the invention can be used for improving drought resistance of potato varieties, can be applied to drought resistance breeding of other crops (such as tomatoes, tobacco, rice and the like) through a genetic engineering technology, and provides important gene resources and effective technical means for coping with global drought environments and guaranteeing grain safety.

Inventors

  • WANG ZEMIN
  • JIN XIN
  • WEI YUXI
  • LI XUAN
  • HOU LELE
  • TIAN MIAO
  • XUE RUIDONG
  • WANG CHENGZHOU
  • AN NING
  • Ai Qianyi

Assignees

  • 甘肃农业大学

Dates

Publication Date
20260505
Application Date
20251120

Claims (4)

  1. The application of STWRKY60 gene or protein encoded by the STWRKY60 gene in improving drought stress resistance of plants is characterized in that the nucleotide sequence of STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of protein encoded by the STWRKY60 gene is shown as SEQ ID NO. 2; Over-expressing STWRKY gene or raising the level of protein coded by it, raising the resistance of plant to drought stress; The plant is potato.
  2. 2. The application of a recombinant vector, an expression cassette or a transgenic strain containing STWRKY gene in improving drought stress resistance of plants is provided, wherein the nucleotide sequence of the STWRKY gene is shown as SEQ ID NO.1, and the plants are potatoes.
  3. 3. A method for increasing drought stress resistance in a plant, comprising overexpressing STWRKY gene or increasing the level of the protein encoded thereby, thereby increasing drought stress resistance in the plant; The plant is potato; the nucleotide sequence of STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2.
  4. The application of STWRKY60 gene or protein encoded by the STWRKY60 gene in cultivating new drought-enduring plant varieties is characterized in that the nucleotide sequence of STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of protein encoded by the STWRKY60 gene is shown as SEQ ID NO. 2; The plant is potato; Over-expressing STWRKY gene or raising its coded protein level and raising the drought stress resistance of plant.

Description

WRKY transcription factor gene StWRKY and application thereof Technical Field The invention relates to the field of biotechnology, in particular to a WRKY transcription factor gene StWRKY and application thereof. Background With the increase in global climate change, drought has become one of the major abiotic stresses that limit agricultural production and affect grain safety. The stress-resistant gene resource of the plant is excavated, and a drought-resistant new variety is cultivated by a genetic engineering means, so that the method is an effective strategy for coping with the challenge. WRKY transcription factors are a family of plant-specific transcription regulatory proteins that are widely involved in plant response to biotic (e.g., pathogenic bacterial infection) and abiotic (e.g., drought, high salt, low temperature) stresses. It specifically binds to the W-box cis-element of its target gene promoter region through a conserved WRKY domain, thereby exerting a core regulatory function in the stress response network. Currently, studies have reported WRKY gene function in a number of species. For example, zmWRKY106 in maize has been shown to positively regulate drought resistance in plants, and inactivation of OsWRKY5 in rice may contribute to improvement of drought resistance. However, the function of WRKY transcription factors is highly species specific and functionally diverse, and the same WRKY gene may play a distinct role in different species. Potatoes are a globally important food crop whose growth and yield are extremely susceptible to drought stress. Therefore, the research on the potato WRKY transcription factor gene StWRKY and the definition of the role in plant stress-resistant biology are of great significance for revealing the drought-resistant molecular mechanism of the potato and improving the drought resistance of crops through genetic engineering means. Disclosure of Invention The invention aims to provide a WRKY transcription factor gene StWRKY and application thereof, so as to solve the problems in the prior art. In order to achieve the above object, the present invention provides the following solutions: According to one of the technical schemes of the invention, STWRKY gene or the coded protein thereof is applied to improving drought stress resistance of plants, the nucleotide sequence of the STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. The second technical proposal of the invention is the application of the recombinant vector, the expression cassette or the transgenic strain containing STWRKY genes in improving the drought stress resistance of plants. In a third technical scheme of the invention, a method for improving the drought stress resistance of plants is used for over-expressing STWRKY genes or improving the encoded protein level of the STWRKY genes and improving the drought stress resistance of plants. The fourth technical scheme of the invention is the application of STWRKY gene or the coded protein thereof in cultivating new drought-enduring plant varieties, the nucleotide sequence of STWRKY gene is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. Based on the technical scheme, the invention has the following technical effects: By constructing StWRKY60 over-expressed transgenic potato plants and subjecting the transgenic potato plants to drought stress treatment, the transgenic potato plants are found to exhibit slower water loss rate, higher relative leaf moisture content, stronger survival rate and lighter oxidative damage (such as lower H 2O2 content and higher antioxidant enzyme activity) than wild type plants. The recovery growth capacity of the transgenic plant is also obviously better than that of the wild type after rehydration after drought stress. The StWRKY gene provided by the invention can be used for improving drought resistance of potato varieties, can be applied to drought resistance breeding of other crops (such as tomatoes, tobacco, rice and the like) through a genetic engineering technology, and provides important gene resources and effective technical means for coping with global drought environments and guaranteeing grain safety. Drawings FIG. 1 shows cloning of potato StWRKY gene. Note that M is DL 2000 DNA marker;1, the target fragment. FIG. 2 is a double cleavage verification of the overexpression vector pC 2300S-StWRKY. Note that M1 is DL 2000 DNA marker;1,2:Kpn I-BamHI digested plasmid and M2 is DL 15000 DNA marker. FIG. 3 is a genetic transformation of potato. Wherein, A is co-culture of 'AC 142' potato chips, B is 'AC 142' callus, C is over-expression callus differentiation bud D is 'AC 142' rooting screening transformation plant. FIG. 4 is a PCR identification of transgenic plants. M is 2000 marker; "AC142" plant DNA as negative control, +: pC2300S-GFP empty vector as positive control, 1-3: stWRKY60 overexpressing strain. FIG.