CN-121222507-B - Handheld micro-droplet generation device and generation method thereof

CN121222507BCN 121222507 BCN121222507 BCN 121222507BCN-121222507-B

Abstract

The invention discloses a handheld micro-droplet generation device and a generation method thereof. The handheld micro-droplet generating device comprises a chip, a strip-shaped injector conversion head and an injector, wherein one end of the injector conversion head is provided with the chip, the other end of the injector conversion head is connected with the output end of the injector, the chip mainly comprises a silicon substrate and organic glass, a central hole which penetrates through the silicon substrate vertically is arranged in the center of the silicon substrate, the lower end of the silicon substrate is connected with the injector conversion head, the upper end of the silicon substrate is connected with the organic glass in an adhering mode through a plasma bonding process, and the organic glass is of a square layered structure. The handheld micro-droplet generating device can solve the gap between laboratory environment and field application, so that the micro-droplet platform can be deployed in the scenes of bedside diagnosis, field biological monitoring, portable synthesis devices and the like, can be combined with various detection technologies, and can greatly improve the detection efficiency and reduce the professional threshold.

Inventors

TANG ZHENGMIN
WANG YUTING
LIU HAOYU
WANG YONGCHENG

Assignees

良渚实验室

Dates

Publication Date: 20260512
Application Date: 20251201

Claims (6)

1. A hand-held micro-droplet generation device, characterized in that: The chip mainly comprises a silicon substrate and organic glass, wherein a central hole which penetrates through the silicon substrate from top to bottom is arranged in the center of the silicon substrate, the lower end of the silicon substrate is connected with the injector conversion head, and the upper end of the silicon substrate is connected with the organic glass in a sticking way; the chip is one of a round chip and a square chip; The upper surface of the circular boss is coaxially provided with three groups of boss groups, each of the three groups of boss groups comprises a first group of boss groups, a second group of boss groups and a third group of boss groups, which are sequentially arranged from outside the central hole to outside, each of the boss groups comprises a plurality of bosses, the bosses in each of the boss groups are arranged along the circumferential array, the first group of boss groups are arranged at the periphery of the central hole, the bosses in the first group of boss groups and the second group of boss groups are a plurality of arc-shaped bosses which are arranged at equal intervals, and the bosses in the second group of boss groups and the bosses in the first group of boss groups are alternately staggered along the circumferential direction; The square chip comprises a square chip, wherein the lower part of the silicon substrate is of a square structure consistent with the four sides of the organic glass, the upper part of the silicon substrate is of a square boss, the surface of the square boss is provided with a plurality of protrusions with the same height, the sides of the protrusions are smaller than the sides of the square structure of the lower part, the protrusions are in pasting connection with the organic glass, the center of the square boss is provided with a center hole, three groups of protrusion groups are coaxially arranged on the upper surface of the square boss and the center hole, each of the three groups of protrusion groups comprises a fourth group of protrusion groups, a fifth group of protrusion groups and a sixth group of protrusion groups which are sequentially arranged from outside the center hole from inside to outside, each protrusion group comprises a plurality of protrusions, the protrusions in the fourth group of protrusion groups and the fifth group of protrusion groups are arranged along the circumferential direction in an array, the protrusions in the fourth group of protrusion groups are arranged on the periphery of the center hole in an equal interval, the protrusions in the fourth group of protrusion groups of protrusions are in an arc shape, each protrusion group of the protrusions in the fifth group of the protrusion groups of the protrusions is arranged along the circumferential direction alternately, each protrusion group of the fourth group of protrusions is arranged along the strip-shaped protrusions arranged along the periphery of the protrusion column, each protrusion column is arranged along the length of the protrusion column, and the protrusion column is arranged along the periphery of the protrusion column, and the protrusion column is arranged along the length; The device comprises a syringe conversion head, a through hole, a hollow round table-shaped structure, a hollow annular structure, a syringe, a cylindrical boss, a hollow ring-shaped structure and a hollow ring-shaped structure, wherein the round end face of the output end of the syringe conversion head is provided with the cylindrical boss which is provided with the through hole and is used for connecting a chip, the diameter of the boss is consistent with the diameter of a central hole of a silicon substrate of the chip, and the through hole is used for communicating liquid in the syringe conversion head; The injector conversion head further comprises a conversion head reinforcing member, wherein the conversion head reinforcing member is of a hollowed rectangular structure, is nested outside the output end of the injector conversion head and is in close contact with the output end of the injector conversion head, and the conversion head reinforcing member is used for reinforcing connection between the chip and the injector conversion head; the actual depth of the injector conversion head is 16mm, 18mm and 31mm respectively, and the outermost diameter of the small-aperture convex end is 1.45mm; The reinforcement is a hollowed-out frame, the inner layer of the frame is 3.6x3.6mm, the outer layer of the frame is 4.2x4.2mm, the thickness is 0.6mm, the depth is 2.5mm, the reinforcement is sleeved from the small-diameter end of the conversion head of the injector when in use until the reinforcement is completely clamped with the conversion head, and the inner layer of the reinforcement is superfluous to form an area of 3.6x3.6x0.4mm when in clamping.
2. The hand-held micro-droplet appliance of claim 1, wherein: The injector comprises a needle cylinder and a push rod, wherein the needle cylinder is of a cylindrical structure with an inner cavity penetrating up and down, one end of the needle cylinder is provided with the push rod, the other end of the push rod is inserted into the input end of the injector conversion head and fixed, one end of the push rod is arranged in the inner cavity of the needle cylinder and matched with the diameter of the inner cavity to form a sliding pair capable of sliding up and down, and the other end of the push rod extends out of the needle cylinder and is used for receiving external pressure.
3. Use of a handheld micro-droplet generation device according to any of claims 1-2, characterized in that: Application in miniaturized droplet manipulation.
4. A hand-held micro-droplet generation method for use in a hand-held micro-droplet generation device according to any of claims 1 to 2 wherein: S1, inserting one end of a syringe conversion head with a small diameter into a central hole of a chip, adding sample liquid into the syringe conversion head by using a liquid shifter, filling the syringe conversion head with the sample liquid, and then inserting a needle cylinder of the syringe into one end of the syringe conversion head with a large diameter to obtain an assembled device; s2, inserting one end of the assembled device with the chip into a plastic centrifuge tube filled with oil phase, so that the oil phase submerges the chip; S3, aligning the finger with the injection pushing tube, pushing the injection pushing tube to enable sample liquid to flow to the chip through the through hole of the boss of the injector conversion head, and forming emulsion liquid drops wrapped by the oil phase in the oil phase along the outside Zhou Liuchu of the chip; S4, separating out emulsion droplets from the plastic centrifuge tube onto a cell counting plate by using a pipette, and observing by using a microscope.
5. The hand-held method of generating droplets according to claim 4, wherein: The oil phase is mainly composed of HFE7500 fluorinated oil.
6. The hand-held method of generating droplets according to claim 4, wherein: the emulsion droplets were 4-6. Mu.L from the plastic centrifuge tube.

Description

Handheld micro-droplet generation device and generation method thereof Technical Field The invention relates to the technical field of microminiaturized liquid drop control, in particular to a handheld micro-liquid drop generating device and an application method thereof. Background The micro-droplet technology is taken as a core branch of the micro-fluidic field, and has revolutionary potential in the fields of biological detection, drug screening, single-cell analysis, nanomaterial synthesis and the like in recent years. The traditional micro-droplet generation system relies on a large pump valve device and a complex external control system, has the problems of huge volume, complex operation, high cost and the like, and severely limits the application of the traditional micro-droplet generation system in field detection, point of care testing (POCT) and distributed laboratory scenes. Disclosure of Invention In order to solve the problems in the background technology, the invention provides a handheld micro-droplet generation chip and a handheld micro-droplet generation method, which are used for solving the problems of huge volume, complex operation, high cost and the like. The handheld liquid drop chip solves the problems of heavy weight, more consumable materials and low efficiency of the traditional detection equipment through the microminiaturization liquid drop control technology, provides a portable, high-sensitivity, low-cost and high-flux instant detection scheme, and is suitable for the fields of medical treatment, environment, food safety and the like. The technical scheme adopted by the invention is as follows: 1. basic structure of handheld micro-droplet generation device The handheld micro-droplet generating device comprises a chip, a strip-shaped injector conversion head and an injector, wherein one end of the injector conversion head is provided with the chip, the other end of the injector conversion head is connected with the output end of the injector, the chip mainly comprises a silicon substrate and organic glass, a center hole which penetrates through the silicon substrate vertically is arranged in the center of the silicon substrate, the lower end of the silicon substrate is connected with the injector conversion head, the upper end of the silicon substrate is connected with the organic glass in a sticking mode, and the organic glass is of a square layered structure. The chip is one of a round chip and a square chip. The upper part of the silicon substrate is a round boss with the diameter smaller than or equal to the side length of the square structure of the lower part and the surface of the boss is provided with a plurality of equal-height bosses, the bosses are in pasting connection with the organic glass, a central hole is formed in the center of the round boss, three boss groups are coaxially arranged on the upper surface of the round boss and the central hole, each boss group comprises a first boss group, a second boss group and a third boss group which are sequentially arranged from outside the central hole from inside to outside, each boss group comprises a plurality of bosses, the bosses in each boss group are arranged along the circumferential array, the bosses in the first boss group and the second boss group are a plurality of arc-shaped bosses which are arranged at equal intervals, the bosses in the second boss group and the bosses in the first boss group are alternately staggered along the circumferential direction, the bosses in the third boss group are strip-shaped bosses which are arranged along the strip-shaped bosses towards the periphery of the boss. The square chip comprises a square chip body, wherein the lower part of the square chip body is of a square structure which is consistent with the four sides of organic glass, the upper part of the square chip body is provided with a square boss, the sides of the square boss are smaller than the sides of the square structure, the sides of the square boss are equal in height, the square boss is in adhesive connection with the organic glass, a central hole is formed in the center of the square boss, three groups of boss groups are coaxially arranged on the upper surface of the square boss and the central hole, each of the three groups of boss groups comprises a fourth boss group, a fifth boss group and a sixth boss group, the fourth boss group, the fifth boss group and the sixth boss group are sequentially arranged from outside the central hole, each boss group comprises a plurality of bosses, the bosses in the fourth boss group and the fifth boss group are arranged along the circumferential direction in an array mode, the bosses in the fourth boss group are arranged on the periphery of the central hole in an equal interval mode, each boss group of the fifth boss group and each boss group of the boss group is alternately staggered in the circumferential direction, each boss group of the boss group comprises strip-shaped