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CN-121242967-B - Nanometer liposome for relieving and repairing, preparation method and application thereof

CN121242967BCN 121242967 BCN121242967 BCN 121242967BCN-121242967-B

Abstract

The invention relates to a nanometer liposome for relieving and repairing, a preparation method and application thereof. The liposome comprises an oil phase and a water phase with the mass ratio of 1 (1-3), wherein the oil phase comprises the following raw materials, by mass, 0.1-0.5% of ceramide NP, 0.3-3% of erigeron breviscapus extract, 0.2-0.5% of hydroxyectoin, 2-4% of squalane, 0.1-0.5% of di (lauramide glutamine) sodium lysine, 1-3% of hydrogenated lecithin, 1-3% of cholesterol and the balance of absolute ethyl alcohol, and the water phase comprises the following raw materials, by mass, 1-5% of dendrobium nobile extract, 1-3% of glycerol glucoside, 0.5-1% of recombinant fibronectin, 20-40% of glycerol and the balance of deionized water. The soothing and repairing nano lipid can realize the double effects of immediate soothing and long-term repairing.

Inventors

  • PAN WU
  • WANG SHUAI
  • LIAO ZHUO
  • HUANG QUANSHENG
  • HUANG LIQIONG
  • Bao Jixian
  • HAN JIANBIAO

Assignees

  • 皓雨(广州)化妆品制造有限公司
  • 北京恒远佳美生物科技有限责任公司

Dates

Publication Date
20260512
Application Date
20251204

Claims (10)

  1. 1. The soothing and repairing nano liposome is characterized by comprising 1 (1-3) of an oil phase and a water phase, wherein the oil phase comprises, by mass, 0.1-0.5% of ceramide NP, 0.3-3% of a wound healing villus extract, 0.2-0.5% of hydroxyectoin, 2-4% of squalane, 0.1-0.5% of sodium bis (lauramide glutamine) lysine, 1-3% of hydrogenated lecithin, 1-3% of cholesterol and the balance of absolute ethyl alcohol, the water phase comprises, by mass, 1-5% of dendrobium nobile extract, 1-3% of glycerol glucoside, 0.5-1% of recombinant fibronectin, 20-40% of glycerol and the balance of deionized water, and a manufacturer of the wound healing villus extract is He Dai chemical (Shanghai) limited company, and the trade name of the wound healing villus extract is PHYTOFLEUR ANTHYLLIS GL; the preparation method of the dendrobium nobile extract comprises the following steps: s1, cleaning dendrobium nobile, mashing, adding deionized water, adjusting the pH to 4-5, adding a complex enzyme for enzymolysis treatment, and inactivating enzyme to obtain an enzymolysis product, wherein the complex enzyme is a composition of cellulase, protease and pectase; s2, performing ultrasonic extraction and filtration on the enzymolysis product to obtain filtrate and filter residues; S3, adding a phosphorylation reagent into the filtrate obtained in the step S2, regulating the pH to 4-6, and stirring for reaction to obtain a phosphorylated water extract, wherein the addition amount of the phosphorylation reagent is 5-10% of the mass of the filtrate, and the phosphorylation reagent is sodium tripolyphosphate and sodium trimetaphosphate with the mass ratio of (2-3): 1; S4, adding the filter residue obtained in the S2 into absolute ethyl alcohol, adding xylitol anhydride monostearate and diacetyl tartaric acid monoglyceride, carrying out reflux extraction, filtering, and rotationally evaporating the filtrate until no ethyl alcohol exists to obtain an alcohol extract, wherein the adding amount of the xylitol anhydride monostearate is 0.3-0.6% of the mass of the filter residue, and the adding amount of the diacetyl tartaric acid monoglyceride is 0.1-0.3% of the mass of the filter residue; and S5, uniformly mixing the phosphorylated aqueous extract obtained in the step S3 with the alcohol extract obtained in the step S4 to obtain the dendrobium nobile extract.
  2. 2. The soothing and repairing nano liposome according to claim 1, wherein in the preparation step S1 of the dendrobium nobile extract, the feed liquid ratio of the dendrobium nobile to deionized water is 1g:15-20mL, the adding amount of the compound enzyme is 1-3% of the mass of the dendrobium nobile, the mass ratio of the cellulase, the protease and the pectase is 1 (0.5-1.5): 0.5-1.5), the enzymolysis temperature is 30-35 ℃ for 1-3h, and the enzyme deactivation temperature is 80-90 ℃ for 10-15min.
  3. 3. The soothing and repairing nanoliposome according to claim 1, wherein in the preparation step S2 of the dendrobium nobile extract, the power of the ultrasonic extraction is 300-400W, the temperature is 40-50 ℃ and the time is 1-2h.
  4. 4. The soothing and repairing nanoliposome according to claim 1, wherein in the preparation step S3 of the dendrobium nobile extract, the stirring reaction is performed at a rotation speed of 500-600rpm, a temperature of 40-50 ℃ and a time of 20-40min.
  5. 5. The soothing and repairing nanoliposome according to claim 1, wherein in the preparation step S4 of the dendrobium nobile extract, the feed liquid ratio of the filter residue to the absolute ethyl alcohol is 1g:5-10mL.
  6. 6. The method for preparing the soothing and repairing nanoliposome according to any one of claims 1 to 5, comprising the following steps: (1) Mixing the raw materials in the oil phase according to the formula amount, uniformly stirring, transferring to a rotary evaporator, and removing ethanol to obtain an oil phase; (2) Uniformly mixing all raw materials in the water phase according to the formula amount to obtain the water phase; (3) Mixing the water phase and the oil phase according to the formula amount, and shearing at high speed to obtain a pre-emulsion; (4) Homogenizing the pre-emulsion by using a micro-jet homogenizer to obtain the soothing and repairing nano-liposome.
  7. 7. The method for preparing the soothing and repairing nano-liposome according to claim 6, wherein in the step (1), the stirring temperature is 50-60 ℃, the rotating speed is 200-400rpm, and the time is 20-30min.
  8. 8. The method for preparing a soothing and repairing nanoliposome according to claim 6, wherein the high-speed shearing rotation speed is 10000-12000 rpm and the time is 2-5 min in the step (3).
  9. 9. The method for preparing a nanometer liposome for comfort and repair according to claim 6, wherein in the step (4), the microfluidic homogenizer is a micifu microfluidic homogenizer, the homogenizing treatment is performed under a pressure of 50-250MPa for 10-30min.
  10. 10. Use of the soothing and repairing nanoliposome according to any one of claims 1 to 5 for preparing cosmetics.

Description

Nanometer liposome for relieving and repairing, preparation method and application thereof Technical Field The invention relates to the technical field of nanoliposomes, in particular to a soothing and repairing nanoliposome and a preparation method and application thereof. Background The soothing and repairing component often comprises two types of oil solubility (such as ceramide and plant extract) and water solubility (such as ectoine), and the prior art adopts a single solubilization mode, so that the dissolubility of certain components is insufficient, and agglomeration and precipitation are easy to occur. If the active ingredients are not effectively protected, the ingredients are easily degraded by temperature, illumination and oxygen, or react with other ingredients in the storage process, so that the stability is poor and the shelf life is short. The stratum corneum barrier of the skin impedes penetration of liposomes, which have been more localized at the skin surface and are unable to deliver the active ingredient to the dermis. The lack of a targeted delivery mechanism, the active ingredient cannot precisely act on the damaged cells, resulting in low repair efficiency and insufficient efficacy. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide a nanometer liposome for relieving and repairing, a preparation method and application thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The invention provides a soothing and repairing nano liposome which comprises 1 (1-3) of an oil phase and a water phase, wherein the oil phase comprises the following raw materials, by mass, 0.1-0.5% of ceramide NP, 0.3-3% of wound-healing villus extract, 0.2-0.5% of hydroxyectoine, 2-4% of squalane, 0.1-0.5% of sodium di (lauramide glutamine) lysine, 1-3% of hydrogenated lecithin, 1-3% of cholesterol and the balance of absolute ethyl alcohol, and the water phase comprises the following raw materials, by mass, 1-5% of dendrobium nobile extract, 1-3% of glycerol glucoside, 0.5-1% of recombinant fibronectin, 20-40% of glycerol and the balance of deionized water; the preparation method of the dendrobium nobile extract comprises the following steps: s1, cleaning dendrobium nobile, mashing, adding deionized water, adjusting the pH to 4-5, adding a complex enzyme for enzymolysis treatment, and inactivating enzyme to obtain an enzymolysis product, wherein the complex enzyme is a composition of cellulase, protease and pectase; s2, performing ultrasonic extraction and filtration on the enzymolysis product to obtain filtrate and filter residues; s3, adding a phosphorylation reagent into the filtrate obtained in the step S2, adjusting the pH to 4-6, and stirring for reaction to obtain a phosphorylated aqueous extract; s4, adding the filter residue obtained in the step S2 into absolute ethyl alcohol, adding xylitol anhydride monostearate and diacetyl tartaric acid monoglyceride and diglyceride, carrying out reflux extraction, filtering, and rotationally evaporating the filtrate until no alcohol exists to obtain an alcohol extract; and S5, uniformly mixing the phosphorylated aqueous extract obtained in the step S3 with the alcohol extract obtained in the step S4 to obtain the dendrobium nobile extract. In the preparation step S1 of the dendrobium nobile extract, complex enzyme (cellulase + protease + pectase) is adopted to decompose the dendrobium nobile cell wall and internal protein structure in a targeted manner, so that the release rate of active ingredients such as polysaccharide, flavone and the like in the dendrobium nobile is improved, and meanwhile, the small molecular peptide generated by enzymolysis can assist in dissolving and stabilizing subsequent active ingredients. And in the step S2, ultrasonic extraction is carried out on the zymolyte, and the cavitation effect of the ultrasonic accelerates cell disruption, so that the dissolution efficiency of the active ingredients is further improved, and the loss of the active ingredients is reduced. In the step S3, because the filtrate obtained after filtration contains more dendrobium nobile polysaccharides, and the phosphorylating reagent introduces phosphate groups into the dendrobium nobile polysaccharides, so that the surfaces of the dendrobium nobile polysaccharides carry more negative charges. The modification can lead the polysaccharide to form a stable electrostatic adsorption complex with recombinant fibronectin (positive charge), and simultaneously improve the emulsifying property and dynamic stability of an aqueous phase system and avoid layering and aggregation of liposome. In the step S4, the filter residue containing more water-insoluble flavone components is treated by adopting absolute ethyl alcohol as a solvent to improve the effective components in the filter residue, on one hand, diacetyl tartaric acid mono-diglyceride is added to break cell membrane barri