CN-121249916-B - Application of molecular marker related to average daily feeding time character in fattening period of pigs

Abstract

The invention discloses application of a molecular marker related to average daily feed intake time characteristics of a pig fattening period, and relates to the technical field of biology, wherein the application is any one of A1) to A4) for detecting or assisting in detecting the average daily feed intake time of the pig fattening period, A2) for identifying and assisting in identifying the average daily feed intake time of the pig fattening period, A3) for preparing a product for detecting or assisting in detecting the average daily feed intake time of the pig fattening period, A4) for preparing a product for identifying and assisting in identifying the average daily feed intake time of the pig fattening period, and the application of the molecular marker related to the average daily feed intake time characteristics of the pig fattening period discovers the A/C locus at 270462812 th chromosome in Sus_scrofa 11.1 of a pig reference genome, and the locus has a significant influence on the average daily feed intake time of the pig fattening period.

Inventors

• WANG LIGANG
• WANG LIXIAN
• ZHANG LONGCHAO
• HOU XINHUA

Assignees

• 中国农业科学院北京畜牧兽医研究所

Dates

Publication Date

20260508

Application Date

20251201

Claims (5)

1. 1. Use of a molecular marker related to the average daily feeding time profile of a pig during fattening period, characterized in that said use is any one of the following A1 to A4: A1. detecting or assisting in detecting average daily feeding time of pigs in fattening period; A2. Identifying and assisting in identifying average daily feeding time of pigs in fattening period; A3. Preparing a product for detecting or assisting in detecting average daily feeding time of pigs in fattening period; A4. Preparing a product for identifying and assisting in identifying average daily feeding time of pigs in fattening period; the molecular marker corresponds to SNP locus 270462812 on chromosome 1 in pig reference genome Sus_scrofa.Sscrofa11.1, the locus corresponds to nucleotide 119bp in sequence SEQ ID NO. 1, and the nucleotide type is A or C.
2. 2. The use of molecular markers associated with the average daily feed intake time profile of a swine fattening period according to claim 1, wherein the method for identifying or assisting in identifying the average daily feed intake time of a swine fattening period comprises the following steps: s1) extracting genome DNA of a pig to be detected as a template; s2) designing a specific primer aiming at the 119bp locus of the DNA molecule shown in the sequence SEQ ID NO. 1, and carrying out PCR amplification; S3) detecting the genotype of the 119 th bp locus of the pig sequence SEQ ID NO. 1 to be detected; S4) identifying or assisting in identifying average daily feeding time of the fattening period of the pig to be tested according to the genotype obtained in the step S3, wherein the average daily feeding time of the fattening period of the AA genotype individual is larger than that of the AC genotype individual, and the average daily feeding time of the fattening period of the AC genotype individual is larger than that of the CC genotype individual.
3. 3. The use of molecular markers related to average daily feeding time profile of swine according to claim 2, wherein the specific primers in step S2 have the sequences of SEQ ID No. 2 and SEQ ID No. 3, wherein SEQ ID No. 2 is 5'-GCTCTTGTGTCTAAATGGAAACC-3', SEQ ID No. 3 is 5'-GGCTTGTTGTCTGGATGATGA-3'.
4. 4. The application of the molecular marker related to the average daily feeding time character in the fattening period of pigs according to claim 3, which is characterized in that a breeding method for breeding pigs with small average daily feeding time in the fattening period is carried out, the genotype of the pigs to be tested is identified according to the method of claim 3, and the pigs to be tested with the 119bp site of SEQ ID NO:1 sequence as C homozygote are selected as parents for breeding.
5. 5. The use of molecular markers associated with the average daily feeding time profile of a swine fattening period according to claim 4, wherein the product is a kit.

Description

Application of molecular marker related to average daily feeding time character in fattening period of pigs Technical Field The invention relates to the technical field of biology, in particular to application of a molecular marker related to average daily feeding time characteristics of a pig in a fattening period. Background In the pig raising industry, the feed cost ratio exceeds 60%, the Feed Conversion Rate (FCR) is a core index for determining raising profit, and researches show that the average daily feeding Time (TFD) in the fattening period of pigs is positively related to the feed conversion rate, namely, the shorter the average daily feeding time is, the higher the feed conversion rate is, the feeding behavior feature is incorporated into the breeding selection index, and the selection reaction of the feed conversion rate can be improved by 4% (reference document ：Kavlak AT, Strandén I, Lidauer MH, Uimari P. Estimation of social genetic effects on feeding behaviour and production traits in pigs. Animal. 2021 Mar;15 (3):100168. doi: 10.1016/j.animal.2020.100168.）. At present, the measurement of average daily feeding time depends on special automatic measurement equipment, the equipment is high in price and long in measurement period span, so that the breeding cost is obviously increased, the actual operation difficulty is high, along with the development of molecular biotechnology and pig genome correlation analysis technology, the possibility is provided for excavating the average daily feeding time related molecular marker and realizing accurate and efficient breeding, but until the present, no molecular marker related report aiming at the average daily feeding time in the fattening period of pigs exists, and therefore, the development of specific molecular markers is required to meet the breeding practice demands. Disclosure of Invention The invention aims to provide application of a molecular marker related to average daily feeding time characteristics in a fattening period of pigs so as to solve the problems in the background technology. In order to achieve the aim, the invention provides the following technical scheme that the application of the molecular marker related to the average daily feeding time character in the fattening period of the pigs is any one of the following A1 to A4: A1. detecting or assisting in detecting average daily feeding time of pigs in fattening period; A2. Identifying and assisting in identifying average daily feeding time of pigs in fattening period; A3. Preparing a product for detecting or assisting in detecting average daily feeding time of pigs in fattening period; A4. Preparing a product for identifying and assisting in identifying average daily feeding time of pigs in fattening period; the molecular marker corresponds to SNP locus 270462812 on chromosome 1 in pig reference genome Sus_scrofa.Sscrofa11.1, the locus corresponds to nucleotide 119bp in sequence SEQ ID NO. 1, and the nucleotide type is A or C. Further, the method for identifying or assisting in identifying average daily feeding time of the fattening period of the pigs is characterized by comprising the following steps: s1) extracting genome DNA of a pig to be detected as a template; s2) designing a specific primer aiming at the 119bp locus of the DNA molecule shown in the sequence SEQ ID NO. 1, and carrying out PCR amplification; S3) detecting the genotype of the 119 th bp locus of the pig sequence SEQ ID NO. 1 to be detected; S4) identifying or assisting in identifying average daily feeding time of the fattening period of the pig to be tested according to the genotype obtained in the step S3, wherein the average daily feeding time of the fattening period of the AA genotype individual is larger than that of the AC genotype individual, and the average daily feeding time of the fattening period of the AC genotype individual is larger than that of the CC genotype individual. Further, the specific primer in the step S2 has the sequences of SEQ ID NO. 2 and SEQ ID NO. 3, wherein SEQ ID NO. 2 is 5'-GCTCTTGTGTCTAAATGGAAACC-3', SEQ ID NO. 3 is 5'-GGCTTGTTGTCTGGATGATGA-3'. Further, a breeding method for breeding pigs with small average daily feeding time in fattening period is adopted, the genotypes of the pigs to be tested are identified according to the method, and the pigs to be tested with the 119bp site of SEQ ID NO. 1 sequence as C homozygote are selected as parents for breeding. Further, the product is a kit. The invention provides application of a molecular marker related to average daily feed intake time characteristics of a pig fattening period, which has the beneficial effects that the invention discovers the A/C site at 270462812 th position of chromosome 1 in a pig reference genome Sus_scrofa.Sscoffa 11.1, the site has obvious influence on the average daily feed intake time of the pig fattening period, a method for identifying or assisting in identifying the average daily feed intake time of t