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CN-121264471-B - Application of micafungin in preparation of drugs for inhibiting fungi

CN121264471BCN 121264471 BCN121264471 BCN 121264471BCN-121264471-B

Abstract

The invention discloses an application of micafungin in preparing a medicine for inhibiting fungi, and an application of micafungin and trifloxystrobin combined medicine in preparing a medicine for inhibiting fungi, in particular to an inhibition effect on rice blast germs. The research of the invention shows that micafungin has an inhibitory effect on rice blast bacteria. The micafungin and the trifloxystrobin are combined for use, and a synergistic effect exists between the micafungin and the trifloxystrobin, so that the drug resistance of the trifloxystrobin-resistant rice blast can be overcome, and the antibacterial effect on the rice blast can be improved. The research of the invention shows that the micafungin has broad-spectrum antifungal effect, and has inhibition effect on other fungi such as botrytis cinerea, bakanae fungus, and flax fungus besides rice blast fungus.

Inventors

  • KOU YANJUN
  • ZHANG YING
  • SHI HUANBIN
  • QIU JIEHUA
  • CHEN ZHENGTING
  • CAI LIANG

Assignees

  • 中国水稻研究所

Dates

Publication Date
20260508
Application Date
20251208

Claims (7)

  1. 1. Application of micafungin and trifloxystrobin combined drug in preparing drugs for inhibiting fungi; the fungus is Pyricularia oryzae (Magnaportheoryzae).
  2. 2. The use according to claim 1, wherein the Pyricularia oryzae is a trifloxystrobin resistant Pyricularia oryzae.
  3. 3. The use according to claim 1, wherein micafungin is used at a concentration of 0.1 to 50 μm.
  4. 4. The use according to claim 3, wherein micafungin is used at a concentration of 1 to 50 μm.
  5. 5. The use according to claim 4, wherein micafungin is used at a concentration of 1 μm and trifloxystrobin is used at a concentration of 0.025 μm.
  6. 6. A method for controlling rice blast, characterized in that a combination of micafungin and trifloxystrobin is applied to rice to be controlled for rice blast.
  7. 7. The method for controlling rice blast according to claim 6, wherein micafungin is used at a concentration of 1. Mu.M and trifloxystrobin is used at a concentration of 0.025. Mu.M.

Description

Application of micafungin in preparation of drugs for inhibiting fungi Technical Field The invention belongs to the technical field of rice blast prevention and control, and particularly relates to application of micafungin in preparation of a medicine for inhibiting fungi. Background The rice blast is a disease seriously threatening the production of rice caused by rice blast bacteria (Magnaporthe oryzae), which can lead to the massive reduction of the yield of the rice and affect the global grain safety. By screening effective control compounds, we can find a new method for controlling rice blast, reduce the dependence on traditional chemical pesticides, delay the drug resistance of common bactericides and promote the development of environment-friendly agriculture. The application of the high-efficiency and low-toxicity control compound can obviously reduce crop loss, improve yield and reduce the influence on an ecological system. Micafungin (Micafungin), CAS number 235114-32-6, is an antifungal agent. The chemical structural formula is as follows: It is mainly used in the medical field, in particular for the problem of fungal infections in human health. Currently, micafungin is mainly used to treat a variety of fungal infections caused by yeasts or molds, including candida (blood infections caused by candida), intraperitoneal infections (e.g., peritonitis), invasive aspergillosis, and the like. One of the advantages is good tolerance and relatively few side effects. For example, a pharmaceutical composition containing an echinocandin antifungal agent micafungin, publication No. WO2012103801A1, and a method for preparing and using the same. The invention application with publication number CN111057130A discloses high-purity micafungin or salt thereof, a preparation method and application thereof. Both of the above prior art disclosures disclose the use of micafungin for the preparation of a medicament for the prevention or treatment of human diseases caused by candida or aspergillus infections. Micafungin acts mainly on a key component in the fungal cell wall, namely beta- (1, 3) -D-glucan, which is abundant in the cell wall of human pathogenic fungi (e.g. candida, aspergillus). Therefore, although micafungin is applied medically as an antifungal agent, it has not been confirmed to be directly useful for controlling plant diseases within the current research scope. Disclosure of Invention The invention provides application of micafungin in preparing a medicine for inhibiting fungi aiming at the defects in the prior art. The invention firstly discloses application of micafungin in preparing a medicine for inhibiting fungi. Preferably, the fungus is Pyricularia oryzae (Magnaporthe oryzae), botrytis cinerea (Botrytis cinerea), phyllostachys cinerea (Fusarium fujikuroi), pedalia officinalis (Sclerotinia sclerotiorum). More preferably, the fungus is Pyricularia oryzae, and the Pyricularia oryzae is Pyricularia oryzae resistant to trifloxystrobin. Preferably, micafungin is used at a concentration of 0.1 to 50 μm. More preferably, micafungin is used at a concentration of 1 to 50 μm. The invention also discloses application of the micafungin and trifloxystrobin combined drug in preparing a drug for inhibiting fungi. Preferably, the fungus is Pyricularia oryzae, botrytis cinerea, phyllostachys nigra, and Pedalus. More preferably, the Pyricularia oryzae is Pyricularia oryzae with trifloxystrobin resistance. The invention also discloses a medicine for inhibiting fungi, and the active ingredients comprise micafungin and trifloxystrobin. The invention also discloses a method for preventing and treating rice blast, which is to apply micafungin or the combination of micafungin and trifloxystrobin to the rice to be prevented and treated. Preferably, the concentration ratio of micafungin to trifloxystrobin is 40:1. More preferably, micafungin is used at a concentration of 1. Mu.M and trifloxystrobin is used at a concentration of 0.025. Mu.M. The research of the invention shows that micafungin has an inhibiting effect on rice blast germs. The micafungin and the trifloxystrobin are combined for use, and a synergistic effect exists between the micafungin and the trifloxystrobin, so that the drug resistance of the trifloxystrobin-resistant rice blast can be overcome, and the antibacterial effect on the rice blast is improved. The research of the invention shows that the micafungin has broad-spectrum antifungal effect, and has inhibition effect on other fungi such as botrytis cinerea, bakanae disease and flax besides rice blast fungus. Drawings FIG. 1 is a graph showing the results of the test for the inhibition of Pyricularia oryzae by micafungin at different concentration gradients in example 1, wherein A in FIG. 1 is a graph showing the results of plate culture and B in FIG. 1 is the results of inhibition. FIG. 2 is a graph showing the results of the pathogenicity test of micafungin inhibiting Pyricularia oryzae in differe