CN-121287537-B - Preparation and application of anti-dandruff composition containing kuh-seng polypeptide

Abstract

The invention relates to the technical field of scalp care, and particularly provides an anti-dandruff composition containing kuh-seng polypeptides, and a preparation method and application thereof. The anti-dandruff composition comprises kuh-seng polypeptide SoFla-I, salicylic acid, selenium disulfide, glutathione, nicotinamide and other active components, and is prepared by a specific process. The amino acid sequence of kuh-seng polypeptide SoFla-I is shown as SEQ ID No.1, and the preparation method comprises the steps of freeze drying of kuh-seng root, ultrasonic extraction, ammonium sulfate precipitation, dialysis purification and the like. Experiments show that the composition can effectively inhibit malassezia and significantly improve dandruff and pruritus symptoms, the dandruff score and pruritus score are significantly reduced after 14 days of use, and the satisfaction degree of a subject is high. The composition has high safety and remarkable anti-dandruff effect, and is suitable for shampoo products, scalp care agents and the like.

Inventors

• KONG LINGCHAO
• LI ZHIPAN
• ZHANG QING
• Kong Zidan

Assignees

• 广州锦同生物科技有限公司

Dates

Publication Date

20260512

Application Date

20251212

Claims (5)

1. 1. An anti-dandruff composition containing kuh-seng polypeptide is characterized by comprising kuh-seng polypeptide SoFla-I, salicylic acid, selenium disulfide, glutathione, nicotinamide and a carrier matrix, wherein the amino acid sequence of kuh-seng polypeptide SoFla-I is shown as SEQ ID No. 1; The carrier matrix comprises glycerol, butanediol, water and a surfactant; the mass percentage of the kuh-seng polypeptide SoFla-I is 0.1% -5%; The mass percentage of the salicylic acid is 1% -3%; the mass percentage of the selenium disulfide is 0.5% -2%; The mass percentage of the glutathione is 0.05-0.2%, and the mass percentage of the nicotinamide is 0.1-0.5%.
2. 2. A method of preparing an anti-dandruff composition according to claim 1, comprising the steps of: (a) Extracting kuh-seng polypeptide SoFla-I, including freeze drying of kuh-seng root, ultrasonic extraction, ammonium sulfate precipitation and dialysis purification; (b) Mixing kuh-seng polypeptide SoFla-I with other active ingredients, and treating by nanoemulsion technology to form a uniform dispersion system.
3. 3. The method of claim 2, wherein the nanoemulsion technique comprises encapsulation of glutathione with liposomes and high pressure homogenization at 600-800bar.
4. 4. Use of a composition as claimed in claim 1 in the preparation of a scalp care product which is a scalp care agent.
5. 5. The use according to claim 4, wherein the composition is for inhibiting malassezia with an inhibition of not less than 90%.

Description

Preparation and application of anti-dandruff composition containing kuh-seng polypeptide Technical Field The invention relates to the technical field of scalp care, in particular to preparation and application of an anti-dandruff composition containing kuh-seng polypeptides. Background Dandruff is a common scalp problem mainly caused by excessive proliferation of malassezia, and is accompanied by scalp itching, desquamation and other symptoms, which affect quality of life and mental health. The existing anti-dandruff products depend on chemical synthesis components such as zinc pyrithione, ketoconazole and the like, and have certain effects, but can cause drug resistance, scalp dryness or irritation response after long-term use. For example, selenium sulphide and salicylic acid are common antidandruff agents, but have limited single use and do not improve well on severe dandruff. Natural extracts such as plant polypeptides are paid attention to because of good biocompatibility and small side effects, but the application of kuh-seng polypeptides in the anti-dandruff field has not been systematically reported. Radix Sophorae Flavescentis is known as a traditional Chinese medicinal material and has antiinflammatory and antibacterial activities, but its polypeptide components are extracted and purified, and its application in scalp care has less research. In the prior art, the extraction methods of the polypeptide such as ultrasonic disruption and chromatographic purification are mature, but the specific sequence of the kuh-seng polypeptide and the synergistic effect of the kuh-seng polypeptide and the anti-dandruff component are not explored. In addition, stability and transdermal absorption efficiency of the active ingredient in the composition are key challenges, such as the susceptibility of glutathione to oxidative inactivation, the need for nano-encapsulation technology to enhance stability. The invention improves the bioavailability by innovatively separating kuh-seng polypeptide SoFla-I and compounding with other components (such as salicylic acid and selenium disulfide) and utilizing the nanoemulsion technology, and solves the problems of poor effect durability and insufficient compatibility of the existing anti-dandruff products. Analysis of the background art shows that the development of a natural polypeptide-based efficient and mild anti-dandruff composition has important application value. Disclosure of Invention The invention firstly provides an anti-dandruff composition containing kuh-seng polypeptide, which comprises kuh-seng polypeptide SoFla-I and at least one active ingredient selected from salicylic acid, selenium disulfide, glutathione and nicotinamide, wherein the amino acid sequence of kuh-seng polypeptide SoFla-I is shown as SEQ ID No. 1. In certain embodiments, a carrier matrix comprising glycerol, butylene glycol, water, and a surfactant is also included. In certain embodiments, the kuh-seng polypeptide SoFla-I is 0.1% -5%, preferably 0.5% by mass. In certain embodiments, salicylic acid is also included in an amount of 1% to 3%, preferably 2% by mass. In certain embodiments, selenium sulphide is also included, in a mass percentage of 0.5% to 2%, preferably 1%. In certain embodiments, glutathione and nicotinamide are also included, wherein the glutathione is 0.05% -0.2% by mass, preferably 0.1% by mass, and the nicotinamide is 0.1% -0.5% by mass, preferably 0.3% by mass. The invention also provides a preparation method of the anti-dandruff composition, which comprises the following steps: (a) Extracting kuh-seng polypeptide SoFla-I, including freeze drying of kuh-seng root, ultrasonic extraction, ammonium sulfate precipitation and dialysis purification; (b) Mixing kuh-seng polypeptide SoFla-I with other active ingredients, and treating by nanoemulsion technology to form a uniform dispersion system. In certain embodiments, the nanoemulsion technique comprises encapsulation of glutathione with liposomes and high pressure homogenization treatment at 600-800bar. The invention also provides the use of the above composition in the preparation of a scalp care product selected from the group consisting of shampoo, scalp care or antidandruff agents. In certain embodiments, the composition is used to inhibit malassezia with an inhibition of not less than 90%. Compared with the prior art, the invention has at least the following beneficial effects: (1) The inhibition rate of the composition to malassezia reaches 96.67%, the diameter of a inhibition zone reaches 8.5mm, and the inhibition rate is obviously higher than that of a control composition (73.33%), and the inhibition rate is benefited by the synergistic effect of kuh-seng polypeptide SoFla-I and selenium disulfide and salicylic acid. (2) Clinical tests show that after 14 days of use, the dandruff score (ASFS value) and the pruritus score are obviously reduced, the satisfaction degree of the subjects is high, and the effect is superior to t