CN-121319154-B - Recombinant human III type collagen and preparation method and application thereof

Abstract

The invention relates to the technical field of genetic engineering, and discloses a recombinant human III-type collagen, a preparation method and application thereof, wherein the amino acid sequence of the recombinant human III-type collagen comprises an amino acid sequence shown as SEQ ID NO. 1. Meanwhile, the 100% humanized amino acid sequence design ensures the high affinity of the product and human skin, and reduces the risk of anaphylactic reaction to the greatest extent.

Inventors

• HUANG ZONGQING
• CHEN GAOJUN
• ZHUANG XIAOXIN
• YANG JIANHUA
• YU HONGZHONG

Assignees

• 上海昱菘生物科技有限公司

Dates

Publication Date

20260508

Application Date

20251217

Claims (12)

1. 1. The recombinant human III type collagen is characterized in that the amino acid sequence of the recombinant human III type collagen is shown as SEQ ID NO. 1.
2. 2. A nucleic acid encoding the nucleotide sequence of recombinant human type III collagen according to claim 1.
3. 3. The nucleic acid of claim 2, wherein the nucleotide sequence of the nucleic acid is set forth in SEQ ID No. 2.
4. 4. A recombinant vector comprising the nucleic acid of claim 2 or 3.
5. 5. A recombinant cell comprising the nucleic acid of claim 2 or 3, or a method for producing the recombinant cell comprising transforming a host cell with the recombinant vector of claim 4.
6. 6. A method for preparing recombinant human type III collagen comprising inducing expression of the recombinant cell of claim 5.
7. 7. A collagen product comprising the recombinant human type III collagen of claim 1 and/or the recombinant human type III collagen prepared by the method of claim 6.
8. 8. The collagen product of claim 7, wherein the collagen product is at least one of a pharmaceutical and a daily chemical product.
9. 9. The collagen product of claim 8, wherein the collagen product is a skin care product.
10. 10. Use of at least one of the recombinant human type III collagen according to claim 1, and the recombinant human type III collagen prepared by the method according to claim 6, for the preparation of a collagen product, said collagen product being a daily chemical product.
11. 11. The use according to claim 10, wherein the collagen product is a skin care product.
12. 12. The use according to claim 10 or 11, characterized in that the use comprises the use of preparing at least one of a collagen product having an effect of inhibiting MMP-1 expression in fibroblasts, a collagen product having an effect of promoting HAS2 expression in keratinocytes, and a collagen product having an effect of promoting HAS3 expression in keratinocytes.

Description

Recombinant human III type collagen and preparation method and application thereof Technical Field The invention relates to the technical field of genetic engineering, in particular to recombinant human III type collagen, a preparation method and application thereof. Background Collagen is the most abundant structural protein in animals, widely exists in connective tissues such as skin, bones, tendons, blood vessels and the like, and plays an important role in supporting, repairing and protecting. The III type collagen is a collagen subtype naturally occurring in human body, has the core function of providing soft and elastic properties for tissues, and is more suitable for sensitive muscle repair, wound healing after medical arts and mucous membrane repair compared with other collagens. The recombinant human type III collagen is a bioactive substance synthesized by a genetic engineering technology and has a high consistency with the structure of human type III collagen, and has a huge application prospect in biomedical materials (such as artificial skin and tissue engineering scaffolds), high-end cosmetics (such as moisturizing and anti-aging skin care products), functional foods and medical fields (such as drug carriers and hemostatic materials). Collagen is obtained by two main approaches, including traditional methods derived from animal tissues and biosynthesis methods for recombinant expression of heterologous proteins, wherein animal tissue sources have biological potential safety hazards and large-scale application cannot be realized. The heterologously expressed collagen includes full-length protein, truncated protein and truncated polypeptide of natural collagen. At present, a plurality of enterprises intercept different collagen fragments for recombinant expression to obtain collagen, but a large number of enterprises have the problem of low activity, and do not play a role in or inhibit the expression of part of beneficial target proteins. Disclosure of Invention The invention aims to solve the technical problems of low activity of recombinant collagen and ineffective or inhibited expression of partial beneficial target protein in the prior art, and provides a recombinant human III-type collagen, a preparation method and application thereof, wherein the recombinant human III-type collagen HAS the characteristics of high hydrophilicity, uniform charge distribution and the like, the characteristics greatly improve the transdermal absorption efficiency of the product, so that the product can penetrate deeper into the bottom layer of the skin, and exert better effects, and the recombinant human type III collagen can also remarkably inhibit MMP-1 expression in fibroblasts, remarkably promote HAS2 expression in keratinocytes and remarkably promote HAS3 expression in keratinocytes. In order to achieve the above object, a first aspect of the present invention provides a recombinant human type III collagen having an amino acid sequence as shown in SEQ ID NO. 1. In a second aspect, the invention provides a nucleic acid encoding said recombinant human type III collagen. In a third aspect, the present invention provides a recombinant vector comprising said nucleic acid. In a fourth aspect, the invention provides a recombinant cell comprising said nucleic acid, or a method of producing said recombinant cell comprising transforming a host cell with said recombinant vector. In a fifth aspect, the present invention provides a method for preparing recombinant human type III collagen, comprising performing inducible expression on the recombinant cells. In a sixth aspect, the present invention provides a collagen product comprising said recombinant human type III collagen and/or said recombinant human type III collagen produced by the method. The seventh aspect of the invention provides the use of the recombinant human type III collagen and/or the recombinant human type III collagen prepared by the method in the preparation of a collagen product. Through the technical scheme, the amino acid sequence of the recombinant human III type collagen provided by the invention has the characteristics of high hydrophilicity, uniform charge distribution and the like, and the characteristics greatly improve the transdermal absorption efficiency of the product, so that the recombinant human III type collagen can penetrate into the bottom layer of the skin more deeply and exert better efficacy. Meanwhile, the 100% humanized amino acid sequence design ensures the high affinity of the product and human skin, and reduces the risk of anaphylactic reaction to the greatest extent. The recombinant human III type collagen provided by the invention has wide application prospects in the fields of medicines, skin care products, daily chemicals, biological materials, industrial materials and the like. In addition, the recombinant human type III collagen provided by the invention can also obviously inhibit MMP-1 expression in fibroblasts, ob