CN-121320201-B - Acid stress resistant heavy metal resistant copper fungus strain, application thereof and fungus agent

Abstract

The invention relates to the field of agricultural microorganism and heavy metal pollution prevention and control, and provides an acid stress resistant metal copper-tolerant bacterial strain, application thereof and a bacterial agent. The strain is preserved in China general microbiological culture Collection center (CGMCC) No.36356, has acid resistance property of stable growth in an environment with pH of 3.0-7.0, and has the capability of reducing the concentration of various heavy metal ions such as chromium, manganese, copper, arsenic, cadmium, lead and the like. In the water planting germination test of wheat and rice, the strain can obviously promote root growth, relieve the inhibition effect of various heavy metal stress on root development, and achieve higher removal rate of heavy metal ions. The invention can be used for heavy metal pollution treatment under acidic condition and agricultural green and safe production.

Inventors

• HOU XIAOYANG
• YU ZHIYAN
• FANG RONGQIAN
• WANG ZHIWEI
• CHEN JIAN
• LIN JIE
• LIN WEIQI

Assignees

• 厦门市产品质量监督检验院
• 复旦大学附属中山医院厦门医院

Dates

Publication Date

20260512

Application Date

20251212

Claims (4)

1. 1. The acid stress resistant heavy metal-resistant copper greedy bacterial strain is named heavy metal-resistant copper greedy bacterial (Cupriavidus metallidurans) xm1225, and is preserved in China general microbiological culture collection center (CGMCC) No.36356.
2. 2. Use of an acid stress tolerant, heavy metal tolerant strain of copper greedy according to claim 1, for promoting root growth of plants including wheat and rice.
3. 3. The use of an acid stress tolerant strain of copper greedy with heavy metals according to claim 1, for degrading heavy metals in soil, including one or more of chromium, manganese, copper, arsenic, cadmium and lead.
4. 4. A microbial agent comprising the acid stress resistant, heavy metal tolerant copper greedy bacterial strain according to claim 1.

Description

Acid stress resistant heavy metal resistant copper fungus strain, application thereof and fungus agent Technical Field The invention relates to the field of agricultural microorganism and heavy metal pollution prevention and control, in particular to an acid stress resistant heavy metal-resistant copper fungus strain, application thereof and a fungus agent. Background Soil heavy metal pollution has become a global environmental problem, and constitutes a continuous threat to agricultural production, grain safety and public health. Toxic heavy metals such as arsenic, cadmium, cobalt, chromium, copper, nickel, lead and the like are difficult to degrade in soil, are easy to accumulate in a cultivated land environment for a long time, can enter a food chain through a soil-crop system, and increase the exposure risk of people. Related researches show that a considerable proportion of farmland soil in the global scope is polluted by the heavy metals, the polluted farmland is large in area, the population is large, and the risk of heavy metal pollution is characterized by being widely and hidden. The problem of heavy metal pollution of Chinese soil is particularly remarkable, and the compound characteristic of acidification and heavy metal pollution superposition is shown in the southern area. Acid soil is widely distributed in a south red soil region, a southwest mountain region and a part of facility agriculture regions, and when the pH value of the soil is low, linkage effects such as unbalanced nutrient supply, enhanced heavy metal activation and migration, reduced soil microbial activity and the like are often caused, so that the growth stress of crops is increased, and the safety of agricultural products is reduced. Meanwhile, the acidification of cultivated lands in China has the trend of expanding range and deepening degree, and the acidification process and the heavy metal pollution are mutually overlapped, so that the agricultural safety production in the south area faces more serious challenges. The toxicity of heavy metals has remarkable inhibition effect on crop growth, is usually represented by phenomena of blocked seed germination, reduced germination rate, slow seedling growth and the like, and can induce adverse effects of disturbed cell division, damaged photosynthesis system, disturbed nutrient absorption and transportation, increased oxidative stress, unbalanced water relation and the like at a physiological level. Especially in an acidic environment, the bioavailability of heavy metals is improved, crop damage is more obvious, and the risks of yield reduction, quality reduction and exceeding of agricultural products are increased. Aiming at the treatment of heavy metal pollution of soil, the method mainly comprises physical and chemical restoration, biological restoration, ecological comprehensive restoration and other paths at present. The physicochemical method has the characteristics of high treatment speed and obvious short-term effect, but has the problems of higher cost, easiness in damaging the soil structure or introducing secondary pollution and the like, and is difficult to be suitable for large-area farmland scenes. In contrast, the bioremediation technology utilizes plants, microorganisms or a combination thereof to remove, fix or transform heavy metals, has the advantages of relatively low cost, environmental friendliness, suitability for large-area light and medium-pollution sites and the like, and has become an important development direction of current soil heavy metal remediation. However, the adaptability of the existing bioremediation strain under the condition of acid soil, the stable restoration capability under the coexistence of various heavy metals and the synergistic performance of the existing bioremediation strain on the growth promotion effect of crops still need to be further improved. Disclosure of Invention The invention aims to solve the technical problem of providing a heavy metal-resistant copper greedy fungus strain with acid stress resistance and multiple heavy metal stress resistance, and the embodiment of the invention provides an acid stress-resistant copper greedy fungus strain with heavy metal stress resistance, application thereof and a microbial inoculum In a first aspect, the embodiment of the invention provides an acid stress resistant heavy metal-resistant copper fungus strain named heavy metal-resistant copper fungus (Cupriavidus metallidurans) xm1225, which is preserved in China general microbiological culture Collection center (CGMCC) No.36356 and has a preservation date of 2025, 10 and 27. Preferably, the 16SrDNA sequence of the strain is shown in SEQ ID NO. 1. Preferably, the strain has acid resistance and is capable of growing in an acidic environment at a pH of 3.0 to 7.0. Preferably, the strain is a short rod-shaped, spore-free gram-negative bacterium. In a second aspect, the embodiment of the invention also provides an application of the a