CN-121342948-B - Plant drought-resistant related protein TaDi19-1 and application of coding gene thereof

Abstract

The invention provides a plant drought-resistant related protein TaDi19-1 and application of a coding gene thereof, belonging to the technical field of genetic engineering. The invention provides a plant drought-resistance related protein, a nucleic acid molecule for encoding the plant drought-resistance related protein, application of a primer pair for amplifying the nucleic acid molecule or a biological material containing the nucleic acid molecule in regulation and control of plant drought-resistance related performance, wherein the plant drought-resistance related protein TaDi19-1 contains an amino acid sequence shown as SEQ ID No. 1. The invention constructs an over-expression plant by introducing the coding gene into a wheat genome for over-expression, the over-expression plant has reduced drought resistance compared with a wild plant, and the coding gene is knocked out, so that the constructed mutant has enhanced drought resistance compared with the wild plant. The protein TaDi19-1 or the gene TaDi19-1 can be used for negatively regulating drought resistance of plants, and can be applied to breeding drought-resistant plant varieties.

Inventors

• MAO HUDE
• WANG CHEN
• DU LINYING
• Xing Tongfang

Assignees

• 西北农林科技大学深圳研究院

Dates

Publication Date

20260512

Application Date

20251219

Claims (4)

1. 1. The application of the plant drought-resistance related protein, the nucleic acid molecule encoding the plant drought-resistance related protein or the biological material containing the nucleic acid molecule in regulating and controlling the plant drought-resistance related performance is characterized in that the plant drought-resistance related protein is shown as any one of the following, wherein the amino acid sequence is shown as SEQ ID No.1 or is connected with a label on the basis of the amino acid sequence shown as SEQ ID No. 1; The nucleic acid molecule is shown as any one of fragments consisting of 1 st to 642 th positions in a nucleotide sequence shown as SEQ ID No.2 or a nucleotide sequence shown as SEQ ID No. 2; knocking out the nucleic acid molecules to improve drought resistance related performance of the plants, wherein the plants are wheat.
2. 2. The use according to claim 1, wherein the type of biological material comprises a recombinant vector, an expression cassette, a transgenic cell line, a recombinant bacterium or a recombinant virus comprising the nucleic acid molecule.
3. 3. The use of claim 1, wherein the knockout method comprises a CRISPR-Cas9 method, and the nucleotide sequence of the designed sgRNA is shown in SEQ ID No.5 and SEQ ID No. 6.
4. 4. A method for enhancing drought resistance-related properties in a plant comprising knocking out a nucleic acid molecule; The nucleic acid molecule is shown as any one of fragments consisting of 1 st to 642 th positions in a nucleotide sequence shown as SEQ ID No.2 or a nucleotide sequence shown as SEQ ID No. 2; The plant is wheat.

Description

Plant drought-resistant related protein TaDi19-1 and application of coding gene thereof Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to a plant drought-resistant related protein TaDi19-1 and application of a coding gene thereof. Background Plants encounter various natural environments during the growth process, and some natural disasters often cause a great deal of yield reduction of crops, such as drought, stagnant water, plant diseases and insect pests, and the like. Cultivation of stress-resistant crop varieties is one of the important targets of agricultural science and technology research. An important breeding method is to genetically engineer various stress-resistant genes to crops so as to obtain new varieties of stress-resistant crops. Wheat (Triticum aestivum l.) is also affected by drought as a major source of carbohydrate and protein sources, and is one of the major contributors to wheat yield reduction. Research shows that the key of crop drought tolerance genetic improvement is cloning and utilization of excellent drought tolerance gene. Therefore, the drought-resistant gene excavation of wheat has important significance for cultivating drought-resistant wheat varieties and improving wheat yield. Disclosure of Invention The invention provides a plant drought-resistance related protein TaDi19-1 and application of a coding gene thereof, wherein the TaDi19-1 and the coding gene thereof negatively regulate and control plant drought-resistance related performance. The invention provides a plant drought-resistant related protein, a nucleic acid molecule for encoding the plant drought-resistant related protein, an application of a primer pair for amplifying the nucleic acid molecule or a biological material containing the nucleic acid molecule in regulating and controlling plant drought-resistant related performance, wherein the plant drought-resistant related protein is shown as any one of an amino acid sequence shown as SEQ ID No.1 or a label connected on the basis of the amino acid sequence shown as the SEQ ID No. 1. In one embodiment of the invention, the nucleic acid molecule is as follows: (1) The nucleotide sequence is shown as SEQ ID No. 2; (2) A fragment consisting of positions 1-642 in the nucleotide sequence shown in SEQ ID No. 2; (3) A nucleic acid molecule having more than 70% homology with (1) or (2) and encoding said plant drought-resistance-related protein; (4) A nucleic acid molecule which hybridizes under stringent conditions to the sequence set forth in (1), (2) or (3) and encodes said plant drought-resistance-related protein. In one embodiment of the invention, the primer pair comprises an upstream primer with a nucleotide sequence shown as SEQ ID No.3 and a downstream primer shown as SEQ ID No. 4. In one embodiment of the invention, the type of biological material comprises a recombinant vector, an expression cassette, a transgenic cell line, a recombinant bacterium or a recombinant virus comprising the nucleic acid molecule. In one specific embodiment of the invention, the regulation comprises the steps of reducing the activity or content of the plant drought-resistance related protein and improving the drought-resistance related performance of the plant; reducing the expression level of the nucleic acid molecule or knocking out the nucleic acid molecule, and improving the drought resistance related performance of plants. In one specific embodiment of the invention, the knockout method comprises a CRISPR-Cas9 method, and the nucleotide sequence of the designed sgRNA is shown as SEQ ID No.5 and SEQ ID No. 6. The invention provides a method for improving drought resistance related performance of plants, which comprises the steps of reducing the activity or content of the plant drought resistance related protein, or reducing the expression level of a nucleic acid molecule for encoding the plant drought resistance related protein or knocking out the nucleic acid molecule, wherein the plant drought resistance related protein is shown as any one of an amino acid sequence shown as SEQ ID No.1 or a label connected on the basis of the amino acid sequence shown as the SEQ ID No. 1. In one embodiment of the invention, the nucleic acid molecule is as follows: (1) The nucleotide sequence is shown as SEQ ID No. 2; (2) A fragment consisting of positions 1-642 in the nucleotide sequence shown in SEQ ID No. 2; (3) A nucleic acid molecule having more than 70% homology with (1) or (2) and encoding said plant drought-resistance-related protein; (4) A nucleic acid molecule which hybridizes under stringent conditions to the sequence set forth in (1), (2) or (3) and encodes said plant drought-resistance-related protein. The invention provides application of plant drought-resistance related protein, nucleic acid molecule for encoding the plant drought-resistance related protein, primer pair for amplifying the nucleic acid molecule or biol