CN-121379876-B - Pseudomonas syringae and application thereof in repairing bone cultural relics

Abstract

The invention discloses pseudomonas syringae and application thereof in repairing bone cultural relics. The Pseudomonas syringae (Pseudomonas syringae) strain SARIP-03 has a preservation number of CCTCC NO: M20251626. The invention also provides a biological agent containing the pseudomonas syringae strain and application thereof in inducing calcium carbonate deposition and solidification and/or bone cultural relic reinforcement and restoration, and a bone cultural relic reinforcement and restoration method. The pseudomonas syringae strain screened by the invention has high urease activity and carbonic anhydrase activity, the generation amount of calcium carbonate is high when the strain or biological agent is adopted to induce the deposition of calcium carbonate, the compressive strength of bone cultural relics can be improved, and the pseudomonas syringae strain can be widely applied to various fields of cultural relics protection, building material reinforcement and the like.

Inventors

• ZHAO ZHIJUN
• JIA BINGQI
• ZOU YIJUN
• ZHOU PENG
• XING PING
• JIANG BIAO

Assignees

• 中国科学院上海高等研究院

Dates

Publication Date

20260512

Application Date

20251022

Claims (11)

1. 1. Pseudomonas syringae (Pseudomonas syringae) strain SARIP-03 with a preservation number of CCTCC NO: M20251626.
2. 2. The strain SARIP-03 of claim 1, wherein the strain SARIP-03 has a 16S rDNA sequence as set forth in SEQ ID No. 1.
3. 3. A biological agent comprising the strain SARIP-03 of any one of claims 1-2.
4. 4. The biological agent of claim 3, further comprising sarcina bardans selected from one or more of the strains having accession numbers cctccc No. M2024524, cccc M2025059, and ATCC 11859.
5. 5. The biological agent according to claim 4, wherein the ratio of the number of viable bacteria of the strain SARIP-03 to that of the Balanococcus barbites is (0.5-3): 1.
6. 6. A composition comprising the strain SARIP-03 of any one of claims 1-2 or the biological agent of any one of claims 3-5, and a calcification solution.
7. 7. The composition of claim 6, wherein the calcification solution comprises calcium salt and urea in a molar ratio of 1 (1-3); And/or the ratio of the concentration of the strain SARIP-03 to the concentration of the calcification solution is (1X 10 9 ～10×10 9 ) cfu/mL:1 mol/L.
8. 8. The composition of claim 7, wherein the calcium salt is selected from one or more of calcium chloride, calcium nitrate, calcium lactate, calcium formate, and calcium acetate; And/or, based on the total volume of the calcification solution, the concentration of the calcium salt is 0.5-10 mol/L.
9. 9. Use of a strain SARIP-03 according to any one of claims 1-2 or a biological agent according to any one of claims 3-5 or a composition according to any one of claims 6-7 for inducing calcium carbonate deposition cure, and/or bone relic reinforcement, and/or bone relic repair.
10. 10. A method for reinforcing or repairing bone relics, comprising the step of treating bone relics with a strain SARIP-03 according to any one of claims 1-2 or a biological agent according to any one of claims 3-5 or a composition according to any one of claims 6-7.
11. 11. The method of claim 10, wherein the bone relics are selected from the group consisting of unearthed ivory.

Description

Pseudomonas syringae and application thereof in repairing bone cultural relics Technical Field The invention belongs to the technical field of cross science of microorganism and material reinforcement engineering, and particularly relates to pseudomonas syringae and application thereof in repairing bone cultural relics. Background The microorganism-induced calcium carbonate deposition (MICP) curing technology exhibits unique application value in various fields by virtue of its excellent ecological compatibility. In the field of building engineering, for example, the MICP curing technology can effectively improve the strength and stability of the foundation and soil on the premise of not damaging ecological balance, and in the field of hydraulic engineering, the MICP curing technology can assist in anti-seepage treatment, and the generated calcium carbonate sediment can effectively block the water flow permeation path, so that the anti-seepage performance of the hydraulic engineering is improved. Precisely because of these remarkable advantages, MICP curing technology has been widely focused and studied in depth, and has shown a broad development prospect. The mechanism of MICP solidification technology includes photosynthetic organism induced deposition of calcium carbonate, sulfate reducing bacteria induced deposition of calcium carbonate, nitrogen cycle induced deposition of calcium carbonate, and other biochemical action induced deposition of calcium carbonate. Among them, the action of nitrogen circulation induced calcium carbonate deposition is particularly critical, and it further includes urea degradation action deposition mechanism and denitrification action deposition mechanism, in which the mode of urea degradation action deposition is most common, and this is beneficial to that its principle is intuitive, easy to control and high in efficiency, and can produce a large quantity of carbonate precipitates in a short time. During the deposition of urea degradation, typical microorganisms such as sarcina barbita produce a key mineralizer, urease, through metabolic activity. When the concentration of urea in the environment is high, urease secreted by bacteria catalyzes the hydrolysis of urea molecules to produce ammonia and carbamic acid. The carbamic acid then spontaneously hydrolyzes, converting rapidly to a molecule of carbonic acid and a molecule of ammonia. After ammonia is dissolved in water, the pH value of the solution is increased, and the alkalinity of the system is promoted to rise. The increase in alkalinity promotes the conversion of dissolved inorganic carbon in the water to carbonate ions, resulting in a significant increase in their concentration. If a source of calcium ions is present in the environment, a calcium carbonate precipitate may be formed. Meanwhile, the surface of the microbial cells often contains a large number of electronegative functional groups, so that Ca < 2+ > in the solution can be effectively adsorbed. When the adsorbed calcium ions meet with the surrounding enriched carbonate, the cells of the bacteria are used as nucleation sites, and calcium carbonate crystals are gradually formed. The finally precipitated crystals can realize reinforcement and repair effects on the material. Urease and carbonic anhydrase play a central role in the process of strengthening calcium carbonate precipitation induced by microorganisms. Urease can catalyze urea to decompose to form ammonia and carbon dioxide, while carbonic anhydrase can promote the solubility of carbon dioxide in water, thereby promoting the conversion thereof. The prior researches focus on common strains such as sarcina bardans, bacillus mucilaginosus and the like. Among them, balanococcus bardanus has high urease activity but slightly low carbonic anhydrase activity, and is often combined with other strains such as bacillus mucilaginosus, bacillus subtilis and the like to perform calcium carbonate deposition. However, bacillus subtilis has high activity of carbonic anhydrase but does not have urease activity, so that the effect is poor when calcium carbonate deposition or solidification of bone cultural relics is carried out. Disclosure of Invention In view of the above-mentioned drawbacks of the prior art, the present invention aims to provide pseudomonas syringae and its application in repairing bone cultural relics, so as to solve the problems existing in the prior art. In order to achieve the above purpose, the present invention adopts the following technical scheme. The first aspect of the invention protects a pseudomonas syringae (Pseudomonas syringae) strain SARIP-03 with the preservation number of CCTCC NO: M20251626. Another aspect of the invention provides a biological agent comprising strain SARIP-03 as described above. Another aspect of the invention protects a composition comprising a strain SARIP-03 as described above or a biological agent as described above, and a calcification solution. Another as