CN-121380184-B - ZmKOB1 gene and application thereof in regulation and control of lodging resistance of corn

Abstract

The invention discloses ZmKOB gene and application thereof in regulation and control of lodging resistance of corn, belonging to the field of plant biotechnology breeding. The corn ZmKOB gene provided by the invention has the function of thickening plant stems and/or enhancing lodging resistance after being highly expressed in plants or being knocked out of a uORF element of the ZmKOB gene, and the gene resource and the application method thereof have wide application prospects in the field of corn breeding.

Inventors

• WANG HAIYANG
• Jia Yingxue
• HE GANG
• WANG BAOBAO
• TAO YOUPENG
• ZHOU XINAN
• CHANG LIGUO
• XIA ZHANCHAO
• ZHAO YUELEI
• YANG DI

Assignees

• 中国农业科学院生物技术研究所

Dates

Publication Date

20260512

Application Date

20251224

Claims (8)

1. 1. A method of producing a maize plant having a phenotype of increased stalk thickening and/or lodging resistance, the method comprising the steps of: (a) The expression quantity or the protein content of the functional gene ZmKOB1 in the corn plant is improved; (b) Obtaining at least one seed of the maize plant produced in step (a); The polynucleotide sequence of the functional gene ZmKOB is selected from one of the following sequences: (c) The polynucleotide sequence shown as SEQ ID No. 1 or 2, or (D) The coding amino acid sequence of the polypeptide is shown as SEQ ID No.3, or (E) A polynucleotide sequence complementary to any one of sequences (c) - (d).
2. 2. The method of claim 1, wherein said increasing the expression level of functional gene ZmKOB in a maize plant comprises introducing into a maize plant an overexpression vector of functional gene ZmKOB1, or knocking out the uORF element of ZmKOB gene, the nucleotide sequence of which is shown as SEQ ID No. 14, to increase the expression level or protein content of ZmKOB 1.
3. 3. The method according to claim 1 or 2, wherein the stem thickening comprises an increase in the diameter of the long axial surface of the stem and/or an increase in the diameter of the short axial surface of the stem.
4. 4. The method of claim 1 or 2, wherein the enhanced lodging resistance comprises a 40 cm thrust enhancement from the ground, a 20 cm thrust enhancement from the ground and/or a 20 cm stalk penetration enhancement from the ground, a reduced incidence of lodging.
5. 5. The method of claim 2, wherein the overexpression vector further comprises a promoter operably linked to the nucleotide sequence of the functional gene and increasing the expression of the functional gene.
6. 6. The method of claim 5, wherein the promoter is CaMV 35S, ubiquitin, or Actin1 promoter, or natural variation using ZmKOB A promoter.
7. 7. The method of claim 2, wherein the knockout is a CRISPR/Cas9, CRISPR/Cas12i3, CRISPR/Cas12j, CRISPR/Cas3C, CRISPR/cpf1 knockout.
8. 8. Use of the method according to any one of claims 1-7 for the production of a maize plant with increased stalk thickening and/or lodging resistance.

Description

ZmKOB1 gene and application thereof in regulation and control of lodging resistance of corn Technical Field The invention belongs to the field of plant biotechnology breeding, and in particular relates to a method for regulating and controlling the thickness and lodging resistance of cornstalk by utilizing ZmKOB gene over-expression or generating functional loss mutation and application thereof. Background In recent years, due to the reasons of deterioration of climate environment, aggravation of various adversity stresses and disasters, large-scale application of nitrogenous fertilizer, promotion of close planting cultivation and the like, the situation of corn lodging hazard is increasingly severe, and lodging has become a main limiting factor of high and stable yield of current corn. Corn lodging is a phenomenon that corn roots or stalks are bent or broken due to external force, and the damage is mainly shown in that 1) lodging disturbs the spatial order of leaves, so that plants collide to damage leaf tissues, photosynthetic efficiency of the plants is weakened, yield is affected, 2) lodging destroys a rhizome transmission and conduction system, nutrient, moisture and photosynthetic product transportation is affected, yield is reduced, 3) lodging can cause spike germination, spike disease is aggravated, corn quality is affected, 4) lodging can cause disorder of plant arrangement, and harvesting difficulty and cost are greatly increased. Existing statistics show that corn lodging can result in 15-50% yield loss, and even in severe cases, corn is dead, with about 108kg/hm yield loss per 1% increase in corn lodging rate. Investigation also shows that, among all traits including yield, lodging resistance is the trait of greatest concern to farmers and is the primary reference factor for farmers to select varieties. Therefore, good lodging resistance is a primary breeding goal for breeding new corn varieties. Disclosure of Invention All references mentioned herein are incorporated herein by reference. Unless defined to the contrary, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Unless indicated to the contrary, the techniques used or referred to herein are standard techniques well known to those of ordinary skill in the art. The materials, methods, and examples are illustrative only and not intended to be limiting. According to the embodiment of the application, through series of experimental researches, the expression quantity of ZmKOB gene is improved in corn plants, the corn plants with the improved expression quantity have unexpected technical effects, the plants with the improved expression quantity have a phenotype of increased stalk thickening and/or enhanced lodging resistance, wherein the increased stalk thickening comprises increased stalk long axis surface diameter and/or increased stalk short axis surface diameter, the enhanced lodging resistance means increased thrust of 40 cm from the ground, increased thrust of 20cm from the ground and/or enhanced stalk puncture force of 20cm from the ground, and the gene resources and functions have great significance for corn breeding. Embodiments of the present application provide a method of producing a maize plant having a phenotype of increased stalk thickening and/or lodging resistance, the method comprising the steps of: (a) The expression quantity or the protein content of the functional gene ZmKOB1 in the corn plant is improved; (b) Obtaining at least one seed of the maize plant produced in step (a). Optionally, the increasing the expression level of the functional gene ZmKOB in the maize plant comprises introducing an overexpression vector of the functional gene ZmKOB1 into the maize plant, or knocking out the uORF element of the ZmKOB gene to increase the expression level or protein content of ZmKOB 1. Optionally, the stem thickening includes an increase in the diameter of the long axial surface of the stem and/or an increase in the diameter of the short axial surface of the stem. Optionally, the lodging resistance enhancement comprises a 40 cm thrust enhancement from the ground, a 20 cm thrust enhancement from the ground, and/or a 20 cm stalk penetration enhancement from the ground, with a reduced incidence of lodging. Optionally, the polynucleotide sequence of ZmKOB gene is selected from one of the following sequences: (a) A polynucleotide sequence as shown in SEQ ID No. 1,2, 4 or 6; (b) The encoding amino acid sequence is shown as a polynucleotide sequence shown as SEQ ID No. 3, 5 or 7; (c) A polynucleotide sequence capable of hybridizing to the polynucleotide sequence of (a) or (b) under stringent hybridization conditions, the overexpression of the polynucleotide sequence exogenous to the maize plant having the function of thickening the plant stalks and/or enhancing lodging resistance; (d) A polynucleotide sequence having at least