CN-121421891-B - Tricholoma matsutake endophytic fungi fermentation oil with whitening effect and preparation method and application thereof

Abstract

The invention relates to tricholoma matsutake endophytic fungi fermentation oil with a whitening effect, and a preparation method and application thereof. The preparation method comprises (1) inoculating Tricholoma matsutake endophytic fungi seed liquid into a fermentation medium for fermentation culture to obtain primary fermentation liquid, and (2) mixing the primary fermentation liquid with vegetable oil and phosphatidylcholine-based eutectic solvent, continuing fermentation culture, and centrifuging to collect upper oil phase to obtain Tricholoma matsutake endophytic fungi fermentation oil. The tricholoma matsutake endophytic fungi fermentation oil prepared by the invention has the transdermal capability of high-level oil-soluble functional components and water-soluble functional components, and has excellent whitening effect.

Inventors

• HUANG CHUJIE
• XU HUIXIAN
• HU LU

Assignees

• 稀物集(广州)生物科技有限公司

Dates

Publication Date

20260508

Application Date

20251231

Claims (5)

1. 1. A preparation method of tricholoma matsutake endophytic fungi fermentation oil with whitening effect is characterized by comprising the following steps: (1) Inoculating the tricholoma matsutake endophytic fungi seed solution into a fermentation medium for fermentation culture to obtain a primary fermentation solution; (2) Mixing the primary fermentation liquid with vegetable oil and phosphatidylcholine-based eutectic solvent, continuing fermentation culture, and centrifugally collecting an upper-layer oil phase to obtain tricholoma matsutake endophytic fungi fermentation oil; The phosphatidylcholine-based eutectic solvent in the step (2) is a mixture of a first eutectic solvent formed by the reaction of palmitoleic acid and phosphatidylcholine and a second eutectic solvent formed by the reaction of myristic acid and phosphatidylcholine, wherein the volume ratio of the first eutectic solvent to the second eutectic solvent is 1:3-3:1; the vegetable oil in the step (2) is a combination of rose-hip oil and sea buckthorn fruit oil in a mass ratio of 1:3-3:1; The tricholoma matsutake endophytic fungi in the tricholoma matsutake endophytic fungi seed liquid in the step (1) is a tricholoma matsutake endophytic fungi XWJ0001 strain, and is classified and named Umbelopsis sp, and the preservation number is CGMCC No.42463.
2. 2. The method of claim 1, wherein the molar ratio of palmitoleic acid to phosphatidylcholine in the first eutectic solvent is 3:1 to 5:1; The molar ratio of the myristic acid to the phosphatidylcholine in the second eutectic solvent is 1:1-3:1.
3. 3. The preparation method of claim 1, wherein the mass ratio of the primary fermentation liquid to the vegetable oil and the phosphatidylcholine-based eutectic solvent in the step (2) is (30-60): 40-60): 1-10.
4. 4. An tricholoma matsutake endophytic fungus fermentation oil prepared by the preparation method of any one of claims 1-3.
5. 5. The use of the tricholoma matsutake endophytic fungi fermentation oil as claimed in claim 4 for preparing products with whitening effect.

Description

Tricholoma matsutake endophytic fungi fermentation oil with whitening effect and preparation method and application thereof Technical Field The invention belongs to the technical field of cosmetics, and particularly relates to tricholoma matsutake endophytic fungi fermentation oil with a whitening effect, and a preparation method and application thereof. Background Tricholoma matsutake is also called Tricholoma matsutake, which is a fungus of Tricholoma genus of Tricholomataceae family, is a famous wild edible fungus and medicinal fungus, and generally grows at the root of pine tree and oak forest in temperate zone and cold temperate zone with higher altitude. As one of the best known higher fungi, the matsutake has tens of active nutrients and has abundant protein and carbohydrate resources. Researches show that the tricholoma matsutake contains a plurality of active substances with important physiological functions, wherein polysaccharide and polyphenol are two core functional components, namely the tricholoma matsutake polysaccharide is taken as a natural high molecular compound, has a plurality of biological activities such as immunoregulation, antioxidation and anti-tumor, has wide application prospects in the fields of health care products and medicines, and the tricholoma matsutake polyphenol is a secondary metabolite rich in phenolic hydroxyl groups, has remarkable free radical scavenging capacity, anti-inflammatory and antibacterial activity, and has important development values in the fields of food preservation, cosmetic raw materials and the like. At present, the acquisition of the polysaccharide and polyphenol of the tricholoma matsutake mainly depends on direct extraction from the fruiting body of the tricholoma matsutake. However, the pine mushroom has very strict requirements on the growing environment, cannot realize artificial large-scale cultivation, and the pine mushroom fruiting body has lower synthesizing efficiency of polyphenol and polysaccharide due to slow growth, metabolic bias structural substances and secondary metabolism, and the traditional extraction technology (such as a solvent extraction method, a supercritical extraction method and the like) has the problems of low extraction rate and high energy consumption, and can possibly damage the structure of the polyphenol and the polysaccharide due to process limitation, so that the utilization rate of the pine mushroom is greatly reduced. The tricholoma matsutake endophyte is a microbial group symbiotic with the tricholoma matsutake for a long time, has a certain similarity between metabolic pathways and the tricholoma matsutake in the process of long-term co-evolution, and has potential capability of synthesizing active ingredients of characteristics of the tricholoma matsutake. Compared with the natural tricholoma matsutake fruiting body, the tricholoma matsutake endophytic fungus has the advantages of high growth speed, mild culture conditions, capacity of realizing large-scale production through fermentation engineering and the like, and can directionally regulate and control the metabolic flow direction by optimizing fermentation process parameters. Therefore, development of a fermentation process of tricholoma matsutake endophytic fungi realizes directional and efficient enrichment of tricholoma matsutake polyphenol and polysaccharide, improves the whitening effect of fermentation products, and becomes one of the technical problems to be solved urgently at present. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide tricholoma matsutake endophytic fungi fermentation oil with whitening effect, and a preparation method and application thereof. In order to achieve the aim of the invention, the invention adopts the following technical scheme: In a first aspect, the invention provides a preparation method of tricholoma matsutake endophytic fungi fermentation oil with whitening effect, which comprises the following steps: (1) Inoculating the tricholoma matsutake endophytic fungi seed solution into a fermentation medium for fermentation culture to obtain a primary fermentation solution; (2) Mixing the primary fermentation liquid with vegetable oil and phosphatidylcholine-based eutectic solvent, continuously fermenting and culturing, centrifuging and collecting upper oil phase to obtain Tricholoma matsutake endophytic fungi fermentation oil. The invention takes the tricholoma matsutake endophytic fungi as an efficient substitute component of the tricholoma matsutake, creatively utilizes the vegetable oil and the phosphatidylcholine-based eutectic solvent to ferment the primary fermentation liquor of the tricholoma matsutake endophytic fungi, wherein the eutectic solvent can regulate and control the metabolism of the tricholoma matsutake endophytic fungi, directionally enrich and synthesize polyphenols and polysaccharide substances, and the phosphatidylcholine-based eutectic solvent can sel