CN-121445650-B - Tricholoma matsutake endophytic fungi fermentation oil and preparation method and application thereof

Abstract

The invention relates to tricholoma matsutake endophytic fungi fermentation oil, and a preparation method and application thereof. The preparation method comprises (1) inoculating Tricholoma matsutake endophytic fungi seed liquid into a fermentation medium for fermentation culture to obtain primary fermentation liquid, and (2) mixing the primary fermentation liquid with vegetable oil and linoleic acid based eutectic solvent, continuing fermentation culture, and centrifuging to collect upper oil phase to obtain Tricholoma matsutake endophytic fungi fermentation oil. The invention uses linoleic acid in the vegetable oil and linoleic acid-based eutectic solvent as a precursor substance of the tricholoma matsutake alcohol, thereby increasing the content of the tricholoma matsutake alcohol in the fermentation product. Meanwhile, the eutectic solvent is added in the fermentation process, the eutectic solvent is used for regulating metabolism, and the fermentation oil has more excellent antioxidant level and relieving and repairing capability in the fermentation product obtained by adopting the method.

Inventors

• XU HUIXIAN
• HU LU
• HUANG CHUJIE

Assignees

• 稀物集(广州)生物科技有限公司

Dates

Publication Date

20260512

Application Date

20251231

Claims (5)

1. 1. A preparation method of tricholoma matsutake endophytic fungi fermentation oil, which is characterized by comprising the following steps: (1) Inoculating the tricholoma matsutake endophytic fungi seed solution into a fermentation medium for fermentation culture to obtain a primary fermentation solution; (2) Mixing the primary fermentation liquor with vegetable oil and an linoleic acid-based eutectic solvent, continuing fermentation culture, and centrifugally collecting an upper-layer oil phase to obtain tricholoma matsutake endophytic fungi fermentation oil; the vegetable oil in the step (2) is a combination of babassu seed oil and grape seed oil with the mass ratio of 1 (0.5-1:5); The linolic acid-based eutectic solvent in the step (2) is obtained by reacting proline and ethyl lactate at 50-90 ℃ and then reacting with linoleic acid; the molar ratio of the proline to the ethyl lactate to the linoleic acid is (1-5): (2-6): (1-5); The tricholoma matsutake endophytic fungi (Umbelopsis sp.) is XWJ0001 strain with a preservation number of CGMCC No. 42463.
2. 2. The method according to claim 1, wherein the mass ratio of the primary fermentation liquid to the vegetable oil/linolic acid group eutectic solvent in the step (2) is (30-60): 40-60): 1-10.
3. 3. The method according to claim 1, wherein the inoculation volume amount of the tricholoma matsutake endophytic fungi seed liquid in the step (1) is 1-10% of the volume of the fermentation medium; The temperature of the fermentation culture in the step (1) is 25-35 ℃, and the time of the fermentation culture is 6-24 h; The temperature of the fermentation culture in the step (2) is 25-35 ℃, and the time of the fermentation culture is 6-24 h.
4. 4. An tricholoma matsutake endophytic fungus fermentation oil prepared by the preparation method of any one of claims 1-3.
5. 5. Use of the tricholoma matsutake endophytic fungi fermentation oil as claimed in claim 4 for preparing products with antioxidant and/or soothing effects.

Description

Tricholoma matsutake endophytic fungi fermentation oil and preparation method and application thereof Technical Field The invention belongs to the technical field of cosmetics, and particularly relates to tricholoma matsutake endophytic fungi fermentation oil and a preparation method and application thereof. Background The tricholoma matsutake alcohol is used as a characteristic fat-soluble active ingredient special for tricholoma matsutake, has remarkable physiological functions of resisting oxidation, resisting inflammation, regulating immunity and the like, can effectively remove free radicals and delay skin aging, and has important application value in the fields of cosmetics, foods and medicines. Currently, the acquisition of matsutake alcohol mainly depends on the extraction from matsutake fruiting bodies. However, the pine mushroom has very strict requirements on the growing environment, cannot realize artificial large-scale cultivation, has low oil synthesis efficiency due to slow growth, metabolic deviation structural substances and secondary metabolism of pine mushroom fruiting bodies, has the problems of low extraction rate and high energy consumption in the traditional extraction technology (such as a solvent extraction method, a supercritical extraction method and the like), and can also cause the structural damage of the pine mushroom due to process limitation, so that the utilization rate of the pine mushroom is greatly reduced. The growth and development of the fungus fruiting body are closely related to those of the endophyte, the endophyte and the fungus fruiting body form symbiotic relationship, obvious damage to host cells is avoided, meanwhile, the endophyte is a rich source of novel bioactive metabolites, and the endophyte not only can produce the pharmaceutically valuable metabolites similar to host plants, but also can directly synthesize the bioactive secondary metabolites. The current fermentation technology of the tricholoma matsutake endophytic fungi still has obvious short plates, namely the synthesis efficiency of the tricholoma matsutake alcohol in the fermentation process is low, directional enrichment cannot be realized, the purity of the tricholoma matsutake alcohol in the product is difficult to meet the high-end application requirement, and meanwhile, the suitability of the fermentation product and oily application scenes is poor, the fermentation product is difficult to be directly applied to products such as skin care essential oil and functional grease, and the further application of the tricholoma matsutake endophytic fungi fermentation product is limited. Therefore, how to optimize the fermentation process of the tricholoma matsutake endophytic fungi, realize the directional efficient enrichment of the tricholoma matsutake alcohol, solve the problems of difficult extraction of fermentation products, insufficient safety, poor suitability of oily scenes and the like, and become one of the technical problems to be solved in the prior art. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide tricholoma matsutake endophytic fungi fermentation oil, and a preparation method and application thereof. In order to achieve the aim of the invention, the invention adopts the following technical scheme: In a first aspect, the invention provides a preparation method of tricholoma matsutake endophytic fungi fermentation oil, which comprises the following steps: (1) Inoculating the tricholoma matsutake endophytic fungi seed solution into a fermentation medium for fermentation culture to obtain a primary fermentation solution; (2) Mixing the primary fermentation liquid with vegetable oil and an linoleic acid-based eutectic solvent (linoleic acid-based DES), continuously fermenting and culturing, and centrifugally collecting an upper-layer oil phase to obtain the tricholoma matsutake endophytic fungi fermentation oil. The invention takes the tricholoma matsutake endophytic fungi as an efficient substitute component of the tricholoma matsutake, creatively adopts the vegetable oil and linoleic acid base eutectic solvent to ferment the primary fermentation liquor of the tricholoma matsutake endophytic fungi, and increases the content of the tricholoma matsutake alcohol in the fermentation product by taking the linoleic acid in the vegetable oil and the linoleic acid base eutectic solvent as a precursor substance of the tricholoma matsutake alcohol. Meanwhile, linoleic acid based eutectic solvent is added in the fermentation process, metabolism is regulated and controlled by the eutectic solvent, and compared with the traditional fermentation mode, the content of active ingredient tricholoma matsutake alcohol in the fermentation product obtained by adopting the method is obviously improved, and the product fermentation oil has more excellent antioxidant and repairing capabilities. Meanwhile, the oil-in-water vesicle is formed by self-assembly of the