CN-121450552-B - Bacillus thuringiensis, microbial inoculum, compound pesticide and application thereof

Abstract

The invention discloses bacillus thuringiensis, a microbial agent, a compound pesticide and application thereof, belonging to the fields of microorganisms and fermentation engineering. The bacillus thuringiensis BT001 is separated from dead insects of diseased noctuid, has strong toxicity, broad insecticidal spectrum, certain salt resistance, heavy metal ion resistance, alkali resistance and high temperature resistance, certain ultraviolet resistance, and can resist various pesticide pesticides, can be used in combination with various pesticide pesticides, and synergistically improves the insecticidal effect, and the bacillus thuringiensis BT001 also has the growth promoting effect. Therefore, the bacillus thuringiensis BT001 disclosed by the invention can be used for preventing and controlling agricultural insect pests and has an important application prospect.

Inventors

• ZHOU BO
• WANG HONG
• WANG LIRONG
• HU ZHURAN
• YANG MEILING
• FAN MEINA
• ZHANG TINGTING
• CHEN HONGMIN
• WANG TENG
• LI RUXUE

Assignees

• 山东碧蓝生物科技有限公司

Dates

Publication Date

20260508

Application Date

20260108

Claims (3)

1. 1. The compound pesticide is characterized by being prepared by compounding bacillus thuringiensis (Bacillus thuringiensis) BT001 and pesticide compounds; The preservation number of the bacillus thuringiensis (Bacillus thuringiensis) BT001 is CCTCC NO: M20252346; the compound pesticide is shown in any one of the following (1) - (3): (1) The bacillus thuringiensis-matrine composite pesticide is prepared by compounding bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation liquor and matrine diluent according to a volume ratio of 1:1; (2) The bacillus thuringiensis-chlorbenzuron composite pesticide is prepared by compounding bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation liquor and chlorbenzuron diluent according to a volume ratio of 1:1; (3) The bacillus thuringiensis-fosthiazate composite pesticide is prepared by compounding bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation liquor and fosthiazate particles; In the bacillus thuringiensis-matrine composite pesticide, the spore concentration in the bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation broth is 1 multiplied by 10 6 CFU/mL-1×10 7 CFU/mL, and the matrine diluent concentration is 5 mg/L-10 mg/L; in the bacillus thuringiensis-chlorbenzuron composite pesticide, the spore concentration in the bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation broth is 1 multiplied by 10 7 CFU/mL, and the concentration of chlorbenzuron diluent is 250 mg/L; In the bacillus thuringiensis-fosthiazate composite pesticide, the viable count of the fermentation liquor of bacillus thuringiensis (Bacillus thuringiensis) BT001 is 5 multiplied by 10 7 CFU/mL, and the fermentation liquor of bacillus thuringiensis (Bacillus thuringiensis) BT001 and 10% fosthiazate particles are compounded according to the ratio of 200mL to 0.2 g.
2. 2. The compound pesticide according to claim 1, wherein the bacillus thuringiensis (Bacillus thuringiensis) BT001 fermentation broth is prepared by the following method: inoculating Bacillus thuringiensis (Bacillus thuringiensis) BT001 into glucose-yeast culture medium, and shake culturing at 37deg.C and 180 r/min for 48 h; The glucose-yeast culture medium comprises glucose 5 g/L, yeast extract 5 g/L, peptone 5 g/L, dipotassium hydrogen phosphate 1 g/L, magnesium sulfate 0.2 g/L and manganese sulfate 0.02 g/L.
3. 3. Use of a compound pesticide according to claim 1 or 2 for controlling plant pests, wherein the plant pests are caused by prodenia litura, spodoptera frugiperda or meloidogyne incognita.

Description

Bacillus thuringiensis, microbial inoculum, compound pesticide and application thereof Technical Field The invention relates to the fields of microorganisms and fermentation engineering, in particular to the technical field of agricultural microorganism prevention and control, and specifically relates to bacillus thuringiensis, a microbial inoculum, a compound pesticide and application thereof. Background In agricultural production, the harm of harmful organisms to crops can be divided into overground and underground harm according to the occurrence parts, different types of harmful organism harm modes and the caused influence are obviously different, and the safety production and the stable yield of the crops are seriously threatened worldwide. Lepidopteran pests are the major overground group that endanger crops, with spodoptera frugiperda (Spodoptera frugiperda), spodoptera litura (Spodoptera litura), asian corn borer (Ostrinia furnacalis Guen ee), and the like being typical representatives. The spodoptera frugiperda and the spodoptera litura have the characteristics of feeding impurities, wide hosts, strong fertility and serious harm, can attack various crops such as corn, soybean, peanut, potato and the like, and the harm is from larval stages. Taking prodenia litura as an example, the newly hatched larvae are always clustered to eat mesophyll, the lower epidermis and mesophyll of the leaves are dispersed and gnawed after 2 years, only the upper epidermis is left, the leaves are in mottled shape due to the increase of the feed intake after 3 years, and the leaves, flowers and fruits can be destroyed completely when the leaves enter the overeating period after 4 years. Corn borers are mainly harmful to gramineous crops such as corn and sorghum. The larvae of the plant are drilled with stalks and cobs to damage internal tissues of the plant, which easily causes reverse folding and insufficient grain grouting, thereby causing serious yield reduction. Plant parasitic nematodes are the major subterranean group of pests that endanger crops, which are important soil-borne pests, and southern root-knot nematodes (Meloidogyne incognita) are particularly typical, with the growing vegetable industry, which is becoming more serious. The infection process of the nematodes has definite rules that oocysts hatch out of second-instar larvae under proper conditions, the larvae swim in soil to search for host root systems, the larvae invade the inside after penetrating tender tissues such as root tips through a mouth needle and migrate to the vicinity of vascular bundles to establish feeding sites, in the process, enzymes secreted by the salivary glands of the nematodes, effector proteins and plant hormone analogues can interfere plant physiological metabolism, induce root cells to form giant cells as long-term nutrition sources, further cause root knot, rot, necrosis and other lesions of the root systems, damage the structure and function of the root systems, influence the absorption and transportation of moisture and nutrients, and finally lead the overground parts of the invaded plants to appear to grow slowly, yellow and dwarf, and dead whole plants in severe cases, thereby causing great yield loss. At present, comprehensive strategies of taking chemical control as a core and biological control and physical control as assistance are generally adopted for controlling lepidoptera pests and nematodes. In the aspect of chemical control, aiming at the important prevention and control of lepidoptera pests in the egg stage and the low-age larva stage, a 20% chlorantraniliprole suspending agent 2000-fold liquid, a 6% high-efficiency cyhalothrin suspending agent 2000-fold liquid and the like are commonly used, and the positions of the back of a leaf, tender stems, heart leaves and the like need to be focused when the pesticide is applied. For nematodes in soil, soil treatment can be carried out by ditch application or hole application before sowing, or application by root irrigation during the growing period of crops. The 5% avermectin microemulsion is commonly used as 1500 times liquid or the 41.7% fluopyram suspending agent is commonly used as 3000 times liquid. In the aspect of biological control, the method can be applied to lepidoptera pests in stages, namely releasing parasitic natural enemies such as yellow trichogramma and dark trichogramma in the egg period, and throwing predatory natural enemies such as heterodera, tortoise plastron and the like in the larva period. Aiming at nematodes, biological control bactericides such as paecilomyces lilacinus, verticillium chlamydia and the like are mainly applied, and eggs and female worm structures of the nematodes are destroyed through spore germination and hypha parasitism, so that population growth is inhibited. In the aspect of physical control, for lepidoptera noctuid adults with chemotaxis, sugar-vinegar liquid trapping or sex trapping cores can be adopted to trap male